Experience

Treeline Biosciences
• United States
• Biotechnology
• 100 - 200 Employee
• Scientist, Structural Biology

  • Aug 2022 - Present


Tufts University School of Medicine

• United States
• Higher Education
• 500 - 600 Employee

• Postdoctoral Scholar

  • Feb 2022 - Aug 2022

  Collaborated with and shadowed a cryo-EM expert to image vesicle deformation mediated by the EBV NEC using a vitrobot and Tecnai F30 Collaborated with and shadowed a cryo-EM expert to image vesicle deformation mediated by the EBV NEC using a vitrobot and Tecnai F30



Tufts University

• United States
• Higher Education
• 700 & Above Employee

• PhD Candidate

  • Sep 2016 - Feb 2022

  The main focus of my project was investigating how the herpes simplex virus type 1 (HSV-1) nuclear egress complex (NEC) interacts with and bends membranes to shuttle nascent capsids out of the nucleus. I purified mutant NECs to test their membrane binding and bending abilities on synthetic membranes. I also established a collaboration with the National Institute of Standards and Technology to train and perform neutron reflectometry experiments to discover how the NEC orients on membranes. I… Show more The main focus of my project was investigating how the herpes simplex virus type 1 (HSV-1) nuclear egress complex (NEC) interacts with and bends membranes to shuttle nascent capsids out of the nucleus. I purified mutant NECs to test their membrane binding and bending abilities on synthetic membranes. I also established a collaboration with the National Institute of Standards and Technology to train and perform neutron reflectometry experiments to discover how the NEC orients on membranes. I managed two other collaborations to investigate protein-membrane interactions with scientists at Cornell (electron spin resonance) and UCLA (small angle x-ray scattering). A second project investigated virus-specific vs conserved features of a purified recombinant Epstein-Barr virus (EBV) NEC through x-ray crystallography and vesicle deformation assays. Here, I operated and maintained the Phoenix crystallization robot to test ~6,000 crystallization conditions for the EBV nuclear egress complex to obtain a hit for crystallization conditions which enabled me to solve the x-ray crystallography structure of the EBV NEC. Show less The main focus of my project was investigating how the herpes simplex virus type 1 (HSV-1) nuclear egress complex (NEC) interacts with and bends membranes to shuttle nascent capsids out of the nucleus. I purified mutant NECs to test their membrane binding and bending abilities on synthetic membranes. I also established a collaboration with the National Institute of Standards and Technology to train and perform neutron reflectometry experiments to discover how the NEC orients on membranes. I… Show more The main focus of my project was investigating how the herpes simplex virus type 1 (HSV-1) nuclear egress complex (NEC) interacts with and bends membranes to shuttle nascent capsids out of the nucleus. I purified mutant NECs to test their membrane binding and bending abilities on synthetic membranes. I also established a collaboration with the National Institute of Standards and Technology to train and perform neutron reflectometry experiments to discover how the NEC orients on membranes. I managed two other collaborations to investigate protein-membrane interactions with scientists at Cornell (electron spin resonance) and UCLA (small angle x-ray scattering). A second project investigated virus-specific vs conserved features of a purified recombinant Epstein-Barr virus (EBV) NEC through x-ray crystallography and vesicle deformation assays. Here, I operated and maintained the Phoenix crystallization robot to test ~6,000 crystallization conditions for the EBV nuclear egress complex to obtain a hit for crystallization conditions which enabled me to solve the x-ray crystallography structure of the EBV NEC. Show less



Harvard Medical School

• United States
• Higher Education
• 700 & Above Employee

• Laboratory Technician

  • Aug 2014 - Aug 2016

  Performed DNA/RNA isolation and quantitation for preparation into Next-Gen sequencing libraries. Executed Next-Generation sequencing runs on MiSeq, HiSeq2500 and NextSeq machines and RT-qPCR gene expression experiments on QuantStudio 7 Led development of an automated protocol on a QIAgility liquid handling robot to accurately pipette next-gen sequencing libraries for quantitation via qPCR Performed DNA/RNA isolation and quantitation for preparation into Next-Gen sequencing libraries. Executed Next-Generation sequencing runs on MiSeq, HiSeq2500 and NextSeq machines and RT-qPCR gene expression experiments on QuantStudio 7 Led development of an automated protocol on a QIAgility liquid handling robot to accurately pipette next-gen sequencing libraries for quantitation via qPCR