Michelle de Oliveira Chain

Postdoctoral Researcher at The National Institute for Bioprocessing Research and Training, NIBRT at NIBRT National Institute for Bioprocessing Research and Training
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Contact Information
us****@****om
(386) 825-5501
Location
Dublin, County Dublin, Ireland, IE
Languages
  • Inglês Full professional proficiency
  • Alemão Elementary proficiency

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Experience

    • Ireland
    • Biotechnology Research
    • 1 - 100 Employee
    • Postdoctoral Researcher at The National Institute for Bioprocessing Research and Training, NIBRT
      • Apr 2022 - Present

      My research focuses on improving cell culture conditions for immunobiologicals using molecular biology and biochemistry techniques. Using high-throughput sequencing methods to identify important targets for genetic engineering. My research focuses on improving cell culture conditions for immunobiologicals using molecular biology and biochemistry techniques. Using high-throughput sequencing methods to identify important targets for genetic engineering.

    • Brazil
    • Biotechnology Research
    • 700 & Above Employee
    • Pharmaceutical Operations and Innovation Analyst
      • Nov 2021 - Mar 2022

      Elisa, protozoan cell culture, organization of the laboratory, feeding control spreadsheets and people management Elisa, protozoan cell culture, organization of the laboratory, feeding control spreadsheets and people management

    • Education Administration Programs
    • 700 & Above Employee
    • PHD Student
      • Mar 2017 - Oct 2021

      Coordination and execution of a research project entitled "Investigation of pro and anti-apoptotic modulators during Trypanosoma cruzi and Leishmania spp infection". Using the following techniques: Primers confection, conventional PCR, Generations of variants, restriction analyses, clonning, SDS-PAGE, Western Blot, replenish competent bacteria stock, qPCR with SYBR Green marking, Mammalian and protozoan cell culture, bacterial transformation, transfection and cell transduction mammalian, immunoprecipitation, immunofluorescence, MTT assay, flow cytometry, panotic staining and DNA sequencing by the Sanger method and new generation by the Illumina platform. Show less

    • Education Administration Programs
    • 700 & Above Employee
    • Master Degree Student
      • Sep 2014 - Oct 2016

      The dissertation entitled "Investigation of the participation of Galectin-3 in the apoptosis process of cells infected by Trypanosoma cruzi" was carried out. Performed at the Molecular Genetics Laboratory at IFRJ. Using the following techniques: SDS-PAGE, Western Blot, Mammalian and protozoan cell culture, MTT assay, flow cytometry, panotic and Giemsa staining, enzymatic activity, immunofluorescence conventional PCR, DNA sequencing by the Sanger method, Generations of variants, restriction analyses and cloning. Show less

    • Scientific iniciation
      • Aug 2012 - Sep 2014

      The completion of the course work entitled "Investigation of the participation of galectin-3 in host cell infection by Trypanosoma cruzi" was carried out in 2014. At the Federal Institute of Education, Science and Technology of Rio de Janeiro. Using the following techniques: SDS-PAGE, Western Blot, Mammalian and protozoan cell culture, flow cytometry, conventional PCR and immunofluorescence

    • Brazil
    • Hospitals and Health Care
    • 300 - 400 Employee
    • Scientific iniciation
      • Jul 2011 - Mar 2012

      A study was carried out involving stem cells in the treatment of Duchenne syndrome. Using the following techniques: Cell culture, immunofluorescence, routines for preparation and sterilization of materials and organization of the laboratory. A study was carried out involving stem cells in the treatment of Duchenne syndrome. Using the following techniques: Cell culture, immunofluorescence, routines for preparation and sterilization of materials and organization of the laboratory.

Education

  • Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro - IFRJ
    Doutorado, Bioquímica e Biologia Molecular
    2017 - 2021
  • Faculdade Unyleya
    Pós-graduação, Gerenciamento de projetos
    2020 - 2021
  • Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro - IFRJ
    Mestrado, Bioquímica e Biologia Molecular
    2014 - 2016
  • Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro - IFRJ
    Graduação, Biologia/Ciências Biológicas - Geral
    2010 - 2014

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