Michelle de Oliveira Chain
Postdoctoral Researcher at The National Institute for Bioprocessing Research and Training, NIBRT at NIBRT National Institute for Bioprocessing Research and Training- Claim this Profile
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Inglês Full professional proficiency
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Alemão Elementary proficiency
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Bio
Experience
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NIBRT National Institute for Bioprocessing Research and Training
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Ireland
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Biotechnology Research
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1 - 100 Employee
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Postdoctoral Researcher at The National Institute for Bioprocessing Research and Training, NIBRT
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Apr 2022 - Present
My research focuses on improving cell culture conditions for immunobiologicals using molecular biology and biochemistry techniques. Using high-throughput sequencing methods to identify important targets for genetic engineering. My research focuses on improving cell culture conditions for immunobiologicals using molecular biology and biochemistry techniques. Using high-throughput sequencing methods to identify important targets for genetic engineering.
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Bio-Manguinhos / Fiocruz
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Brazil
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Biotechnology Research
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700 & Above Employee
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Pharmaceutical Operations and Innovation Analyst
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Nov 2021 - Mar 2022
Elisa, protozoan cell culture, organization of the laboratory, feeding control spreadsheets and people management Elisa, protozoan cell culture, organization of the laboratory, feeding control spreadsheets and people management
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IFRJ - Instituto Federal do Rio de Janeiro
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Education Administration Programs
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700 & Above Employee
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PHD Student
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Mar 2017 - Oct 2021
Coordination and execution of a research project entitled "Investigation of pro and anti-apoptotic modulators during Trypanosoma cruzi and Leishmania spp infection". Using the following techniques: Primers confection, conventional PCR, Generations of variants, restriction analyses, clonning, SDS-PAGE, Western Blot, replenish competent bacteria stock, qPCR with SYBR Green marking, Mammalian and protozoan cell culture, bacterial transformation, transfection and cell transduction mammalian, immunoprecipitation, immunofluorescence, MTT assay, flow cytometry, panotic staining and DNA sequencing by the Sanger method and new generation by the Illumina platform. Show less
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IFRJ - Instituto Federal do Rio de Janeiro
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Education Administration Programs
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700 & Above Employee
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Master Degree Student
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Sep 2014 - Oct 2016
The dissertation entitled "Investigation of the participation of Galectin-3 in the apoptosis process of cells infected by Trypanosoma cruzi" was carried out. Performed at the Molecular Genetics Laboratory at IFRJ. Using the following techniques: SDS-PAGE, Western Blot, Mammalian and protozoan cell culture, MTT assay, flow cytometry, panotic and Giemsa staining, enzymatic activity, immunofluorescence conventional PCR, DNA sequencing by the Sanger method, Generations of variants, restriction analyses and cloning. Show less
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Scientific iniciation
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Aug 2012 - Sep 2014
The completion of the course work entitled "Investigation of the participation of galectin-3 in host cell infection by Trypanosoma cruzi" was carried out in 2014. At the Federal Institute of Education, Science and Technology of Rio de Janeiro. Using the following techniques: SDS-PAGE, Western Blot, Mammalian and protozoan cell culture, flow cytometry, conventional PCR and immunofluorescence
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Instituto Nacional de Traumatologia e Ortopedia
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Brazil
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Hospitals and Health Care
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300 - 400 Employee
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Scientific iniciation
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Jul 2011 - Mar 2012
A study was carried out involving stem cells in the treatment of Duchenne syndrome. Using the following techniques: Cell culture, immunofluorescence, routines for preparation and sterilization of materials and organization of the laboratory. A study was carried out involving stem cells in the treatment of Duchenne syndrome. Using the following techniques: Cell culture, immunofluorescence, routines for preparation and sterilization of materials and organization of the laboratory.
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Education
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Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro - IFRJ
Doutorado, Bioquímica e Biologia Molecular -
Faculdade Unyleya
Pós-graduação, Gerenciamento de projetos -
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro - IFRJ
Mestrado, Bioquímica e Biologia Molecular -
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro - IFRJ
Graduação, Biologia/Ciências Biológicas - Geral