Michael Gerloff
Research Scientist at Max Planck Institute for Molecular Genetics- Claim this Profile
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Bio
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Experience
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Max Planck Institute for Molecular Genetics (MPI-MG)
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Germany
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Research Services
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100 - 200 Employee
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Research Scientist
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Mar 2017 - Present
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Max Delbrück Center
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Germany
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Research Services
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500 - 600 Employee
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PHD Student
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Jul 2016 - Feb 2017
Leutz Lab.Investigation of a tripartite complex including C/EBP Alpha and Beta. Same Methods as in diploma thesis.
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Student Research Assistant
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Oct 2015 - Jun 2016
Leutz Lab. Investigation of a tripartite complex including C/EBP Alpha and Beta. Diploma thesis.Methods:Co-Immunoprecipitation, establishing the Capillary Nano-Immunoassay, Wes, CRISPR/CAS knock out, Electrophoretic Mobility Shift Assay (EMSA), FACS, Live Cell Imaging, Isolation of genomic DNA andProtein, Molecular Cloning, SDS-PAGE, Western Blot, Immunofluorescence, PCR and DNA Sequencing, Cell Culture
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Max Delbrück Center
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Germany
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Research Services
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500 - 600 Employee
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Student Research Assistant
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Feb 2013 - Dec 2014
Walther Birchmeier Lab. Analysis of murine triple negative breast cancer stem cells. Methods: Isolation, culturing, treating with small molecular inhibitors of murine breast cancer stem cells. Investigation of active signal transduction pathways by SDS-PAGE and Western Blot. Immunofluorescence and analysis of tissue sections. Isolation of Protein, RNA, DNA and Histones out of tissue and cells Walther Birchmeier Lab. Analysis of murine triple negative breast cancer stem cells. Methods: Isolation, culturing, treating with small molecular inhibitors of murine breast cancer stem cells. Investigation of active signal transduction pathways by SDS-PAGE and Western Blot. Immunofluorescence and analysis of tissue sections. Isolation of Protein, RNA, DNA and Histones out of tissue and cells
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Max Delbrück Center
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Germany
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Research Services
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500 - 600 Employee
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Student Research Assistant
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Aug 2012 - Dec 2012
Advanced Light Microscopy (ALM) technology platform. Cleaning and maintenance of microscopes, active user support and introduction to Laser Scanning Confocal Microscopes, establishing a STORM System (super resolution microscopy), cell culture, Immunofluorescence. Evaluation of Core Facility Management Software Advanced Light Microscopy (ALM) technology platform. Cleaning and maintenance of microscopes, active user support and introduction to Laser Scanning Confocal Microscopes, establishing a STORM System (super resolution microscopy), cell culture, Immunofluorescence. Evaluation of Core Facility Management Software
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Max Delbrück Center
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Germany
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Research Services
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500 - 600 Employee
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Student Research Assistant
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Oct 2011 - May 2012
Poy Lab. Investigation and analysis of diabetic mice via Immunofluorescence and Laser Scanning Confocal Microscopy. Methods: Tissue sections and analysis via Immunofluorescence, genotyping mice via tail cut biopsies, perfusion of mice by paraformaldehyde. Establishing a semi automated quantification analysis workflow of pancreatic islets in large tile scans. Poy Lab. Investigation and analysis of diabetic mice via Immunofluorescence and Laser Scanning Confocal Microscopy. Methods: Tissue sections and analysis via Immunofluorescence, genotyping mice via tail cut biopsies, perfusion of mice by paraformaldehyde. Establishing a semi automated quantification analysis workflow of pancreatic islets in large tile scans.
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Max Planck Institute for Molecular Genetics (MPI-MG)
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Germany
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Research Services
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100 - 200 Employee
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Student Research Assistant
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Mar 2010 - Jul 2011
Kuss lab. Genetic analysis of autosomal-recessive intellectual disability. PCR and DNA Sequencing, Maintaining and entering information in a genetic family database. Kuss lab. Genetic analysis of autosomal-recessive intellectual disability. PCR and DNA Sequencing, Maintaining and entering information in a genetic family database.
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Freie Universität Berlin
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Germany
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Research Services
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700 & Above Employee
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Student Research Assistant
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Mar 2009 - Dec 2009
Sigrist Lab. Methods: Maintaining and selection of Drosophila strains, managing chemicals, Molecular Cloning. Sigrist Lab. Methods: Maintaining and selection of Drosophila strains, managing chemicals, Molecular Cloning.
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Education
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Freie Universität Berlin
Diplom, Biochemie
Community
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