Experience

ClearNote Health (formerly Bluestar Genomics)

• Lab Services Supervisor

  • Jan 2021 - Present

  San Diego, California, United States

• Research Associate

  • May 2018 - Present

  Greater San Diego Area • Team lead for processing patient and healthy donor blood and tissue samples. • Isolation of cfDNA from processed donor plasma samples. • Characterization and QC of cfDNA for NGS library preparation for use on Illumina platforms. • Operation and maintenance of QiaSymphony liquid handler system. • Patient sample storage and database management.



F1 Oncology

• Contract Research Associate

  • Mar 2017 - May 2018

  San Diego • Transfection, harvest, and concentration of pseudotyped Lenti-virus. • Characterization of virus via western blotting, ELISA, qRT-PCR, and functional titers. • Isolated PBMCs from donor leukopaks and subsequent transduction for CAR-T cell production. • Maintenance of Jurkat, HeLa, Raji, and HEK293 cell lines for downstream CAR-T cell candidate screening. • Production of engineered cell lines constitutively expressing target surface proteins. • Generated numerous vectors for… Show more • Transfection, harvest, and concentration of pseudotyped Lenti-virus. • Characterization of virus via western blotting, ELISA, qRT-PCR, and functional titers. • Isolated PBMCs from donor leukopaks and subsequent transduction for CAR-T cell production. • Maintenance of Jurkat, HeLa, Raji, and HEK293 cell lines for downstream CAR-T cell candidate screening. • Production of engineered cell lines constitutively expressing target surface proteins. • Generated numerous vectors for ectopic expression of candidate targets through restriction enzyme and digestion independent based cloning techniques. • Small-to-large scale DNA isolation and quality control processing for companywide experiments and products Show less



California Polytechnic State University-San Luis Obispo

• United States
• Higher Education
• 700 & Above Employee

• Graduate Research Assistant

  • Jun 2014 - Mar 2017

  San Luis Obispo, California • Designed, cloned, and QC’d mutated LAGLIDADG homing endonucleases in expression vectors. • Transformation of engineered protein vectors into expression and storage bacterial cell lines. • Expression and subsequent column-based purification of engineered proteins. • Characterization of candidate endonucleases via western blotting, time course and enzymatic efficacy assays. • Development and optimization of PCR and cloning strategies used for production. • Buffer and reagent… Show more • Designed, cloned, and QC’d mutated LAGLIDADG homing endonucleases in expression vectors. • Transformation of engineered protein vectors into expression and storage bacterial cell lines. • Expression and subsequent column-based purification of engineered proteins. • Characterization of candidate endonucleases via western blotting, time course and enzymatic efficacy assays. • Development and optimization of PCR and cloning strategies used for production. • Buffer and reagent preparation for lab wide experiments. Show less