Experience

Design Therapeutics
• United States
• Biotechnology Research
• 1 - 100 Employee
• Senior Research Associate

  • May 2023 - Present


Ferring Pharmaceuticals

• Switzerland
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Associate Scientist

  • Feb 2019 - May 2023

  Designed and automated in vitro assays to support hit-to-lead identification of small molecule compound protein-protein interaction (PPI) inhibitors - Developed biochemical assays, such as AlphaLISA and MSD ELISA, starting from identifying reagents to completing the protocol of high quality, robust assays. Facilitated the transfer of such assays to CRO and troubleshooting efforts. - Developed cellular assays to assess compound inhibition of cell binding to protein covered wells, and ran weekly screens of developed small molecule compounds through the assays - Validated CRISPR KO cell line gene expression via qPCR with a Quantstudio 6, and via FACs with a Sartorius iQue3 - Automated biochemical and cellular assays for high throughput screening utilizing liquid handlers and plate washers integrated on a robotics system from HighRes Biosolutions, and included instruments such as Multidrop Combii, Echo Acoustic Dispenser, Agilent Bravo, Biotek EL406 Plate Washer, BlueCatBio BlueWasher - Acquired assay data with a PherastarFSX, and performed analysis with GraphPadPrism and GeneData Screener 18 Designed in vitro assays to assess downstream signaling of GPCR targets to support drug discovery efforts and SAR studies of developed peptide and small molecule compounds - Transfected GPCRs and developed stable cell line clones in adherent cell cultures utilizing FugeneHD kits - Developed cAMP HTRF assays using Cisbio kits to assess stimulation of transfected receptors with test compounds - Developed imaging-based receptor internalization assays using an ImageXpress Micro Confocal Imager Show less



The Scripps Research Institute

• Research Intern

  • Sep 2016 - Jan 2019

  Assisted with research on biomechanical properties of structural proteins on mouse eye lens - Performed mRNA extraction of mouse samples using TRIzol, created cDNA using Qiagen reverse transcription kit, and performed PCR and gel-electrophoresis of cDNA for gene isolation to be sent for sequencing - Extracted proteins from whole mouse tissues and cultured cells, and quantified using Bradford and BCA Assay - Operated a ProteinSimple Wes machine, performed traditional Western Blot analysis of protein samples Show less



University of California San Diego

• United States
• Hospitals and Health Care
• 1 - 100 Employee

• Masters Student

  • Jun 2017 - Dec 2018

  Completed and defended a Master’s Thesis on an independent project with the guidance of the PI researching group 2 innate lymphoid cell signaling and the effects on the pathogenesis of allergic asthma - Experience with mice handling including anesthetization, intranasal challenges, dissections and organ extraction - Isolated single-cell suspensions of granulocytes and PBMCs from mouse organs and carried out FACs staining for analysis of surface markers, nuclear transcription factor expression, and intracellular cytokine production - FACs analysis of single-cell suspensions preformed with an Accuri C6 and a Novocyte flow cytometer - Cultured and stimulated primary immune cells for analysis of cytokine production from cells using ELISA - Analyzed ELISA and flow cytometry data and utilizing FlowJo, Microsoft Excel, and GraphPad Prism softwar Show less