Micaela Orsi
Associate Research Scientist at iTeos Therapeutics- Claim this Profile
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Italian Native or bilingual proficiency
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English Professional working proficiency
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French Professional working proficiency
Topline Score
Bio
Experience
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iTeos Therapeutics
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United States
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Biotechnology Research
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100 - 200 Employee
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Associate Research Scientist
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Aug 2021 - Present
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Université catholique de Louvain
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Belgium
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Research Services
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700 & Above Employee
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PhD
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Oct 2015 - Dec 2020
PhD, Biomedical and Pharmaceutical Sciences, UCLouvain, Brussels, Belgium. Characterization of a peritoneal macrophage subpopulation recruited by particles inducing mesothelioma. Techniques used: - Cell culture of primary cell lines from Wistar rat: o peritoneal macrophages, o splenocytes; o bone-marrow precursors for macrophage differentiation, - Thymidine incorporation assay for cell proliferation assessment, - ATP luciferase assay to test cell viability; - Flow cytometry: o cell surface staining and intracellular cytokine staining, o BrdU and ki-67 staining for cell proliferation assessment, o acquisition and analysis of multiparametric cell samples with BD FACSCanto II Flow Cytometer, o data analysis of FCS files with Flowjo software; - RNA purification and dosage: o RNA extraction with silica-membrane columns (RNAeasy Mini kit) and via liquid/liquid separation (TRIzol® Reagent), o RNA dosage via UV-vis absorption (NanoDrop) and via fluorescence emission (Qubit); - qRT-PCR: o primer design, o preparation of the standards, o acquisition and analysis of the samples; - ELISA assay for cytokine measurement; - GraphPad Prism 9 for statistical analysis and data representation. - certifications for laboratory animal manipulation: o Catégorie B Felasa : “Technicien Animalier” ; o Catégorie C Felasa : “Maître d'Expérience”. - Scientific writing and scientific presentations. Show less
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Student
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Mar 2014 - Mar 2015
Study of the reactivity and biocompatibility of glasses and glass-ceramics created for bone-tissue replacement. Techniques used: - Ultraviolet–visible spectroscopy: o lipid peroxidation measurement by thiobarbituric acid assay, o phenolic and polyphenolic antioxidants measurement by Folin–Ciocalteu assay; - EPR-spin trapping for the detection of Reactive Oxygen Species. Study of the reactivity and biocompatibility of glasses and glass-ceramics created for bone-tissue replacement. Techniques used: - Ultraviolet–visible spectroscopy: o lipid peroxidation measurement by thiobarbituric acid assay, o phenolic and polyphenolic antioxidants measurement by Folin–Ciocalteu assay; - EPR-spin trapping for the detection of Reactive Oxygen Species.
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Intern
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Jul 2013 - Sep 2013
Main tasks : - antibodies screening in animal serum (ELISA and Rosa Bengala tests), - microbiological analysis on food Main tasks : - antibodies screening in animal serum (ELISA and Rosa Bengala tests), - microbiological analysis on food
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Università degli Studi di Torino
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Italy
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Education Administration Programs
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700 & Above Employee
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Student
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Nov 2011 - May 2012
Main tasks: - purification of recombinant proteins via liquid-solid chromatography - enzymatic activity assays (ELISA test ...) Main tasks: - purification of recombinant proteins via liquid-solid chromatography - enzymatic activity assays (ELISA test ...)
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Education
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Université catholique de Louvain
Doctor of Philosophy - PhD, Biomedical and pharmaceutical sciences -
Università degli Studi di Torino
Master's degree, Industrial biotechnology -
Università degli Studi di Torino
Bachelor's degree, Biotechnology -
Liceo Scientifico Giuseppe Peano, Cuneo
High school leaving qualification, scientific studies