Micaela Orsi

Associate Research Scientist at iTeos Therapeutics
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Contact Information
us****@****om
(386) 825-5501
Location
BE
Languages
  • Italian Native or bilingual proficiency
  • English Professional working proficiency
  • French Professional working proficiency

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Experience

    • United States
    • Biotechnology Research
    • 100 - 200 Employee
    • Associate Research Scientist
      • Aug 2021 - Present

    • Belgium
    • Research Services
    • 700 & Above Employee
    • PhD
      • Oct 2015 - Dec 2020

      PhD, Biomedical and Pharmaceutical Sciences, UCLouvain, Brussels, Belgium. Characterization of a peritoneal macrophage subpopulation recruited by particles inducing mesothelioma. Techniques used: - Cell culture of primary cell lines from Wistar rat: o peritoneal macrophages, o splenocytes; o bone-marrow precursors for macrophage differentiation, - Thymidine incorporation assay for cell proliferation assessment, - ATP luciferase assay to test cell viability; - Flow cytometry: o cell surface staining and intracellular cytokine staining, o BrdU and ki-67 staining for cell proliferation assessment, o acquisition and analysis of multiparametric cell samples with BD FACSCanto II Flow Cytometer, o data analysis of FCS files with Flowjo software; - RNA purification and dosage: o RNA extraction with silica-membrane columns (RNAeasy Mini kit) and via liquid/liquid separation (TRIzol® Reagent), o RNA dosage via UV-vis absorption (NanoDrop) and via fluorescence emission (Qubit); - qRT-PCR: o primer design, o preparation of the standards, o acquisition and analysis of the samples; - ELISA assay for cytokine measurement; - GraphPad Prism 9 for statistical analysis and data representation. - certifications for laboratory animal manipulation: o Catégorie B Felasa : “Technicien Animalier” ; o Catégorie C Felasa : “Maître d'Expérience”. - Scientific writing and scientific presentations. Show less

    • Student
      • Mar 2014 - Mar 2015

      Study of the reactivity and biocompatibility of glasses and glass-ceramics created for bone-tissue replacement. Techniques used: - Ultraviolet–visible spectroscopy: o lipid peroxidation measurement by thiobarbituric acid assay, o phenolic and polyphenolic antioxidants measurement by Folin–Ciocalteu assay; - EPR-spin trapping for the detection of Reactive Oxygen Species. Study of the reactivity and biocompatibility of glasses and glass-ceramics created for bone-tissue replacement. Techniques used: - Ultraviolet–visible spectroscopy: o lipid peroxidation measurement by thiobarbituric acid assay, o phenolic and polyphenolic antioxidants measurement by Folin–Ciocalteu assay; - EPR-spin trapping for the detection of Reactive Oxygen Species.

    • Intern
      • Jul 2013 - Sep 2013

      Main tasks : - antibodies screening in animal serum (ELISA and Rosa Bengala tests), - microbiological analysis on food Main tasks : - antibodies screening in animal serum (ELISA and Rosa Bengala tests), - microbiological analysis on food

    • Italy
    • Education Administration Programs
    • 700 & Above Employee
    • Student
      • Nov 2011 - May 2012

      Main tasks: - purification of recombinant proteins via liquid-solid chromatography - enzymatic activity assays (ELISA test ...) Main tasks: - purification of recombinant proteins via liquid-solid chromatography - enzymatic activity assays (ELISA test ...)

Education

  • Université catholique de Louvain
    Doctor of Philosophy - PhD, Biomedical and pharmaceutical sciences
    2015 - 2020
  • Università degli Studi di Torino
    Master's degree, Industrial biotechnology
    2012 - 2014
  • Università degli Studi di Torino
    Bachelor's degree, Biotechnology
    2009 - 2012
  • Liceo Scientifico Giuseppe Peano, Cuneo
    High school leaving qualification, scientific studies
    2004 - 2009

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