Experience

GentiBio

• United States
• Biotechnology
• 1 - 100 Employee

• Senior Associate Scientist

  • Apr 2022 - Present

  Cambridge, Massachusetts, United States Platform Technologies



Obsidian Therapeutics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Associate Scientist

  • May 2021 - Apr 2022

  Process Development • Assessed the quality of gamma retroviral transduction in immune cell lines and primary cells • Extracted, expanded, and engineered primary tumor infiltrating lymphocytes from solid tumors to express a membrane-bound cytokine for enhanced persistence • Optimized the primary expansion protocol for extracting pre-Rapid Expansion Protocol (REP) TILs • Assessed contaminating cells from the pre-REP process using multicolor flow cytometry • Evaluated the… Show more Process Development • Assessed the quality of gamma retroviral transduction in immune cell lines and primary cells • Extracted, expanded, and engineered primary tumor infiltrating lymphocytes from solid tumors to express a membrane-bound cytokine for enhanced persistence • Optimized the primary expansion protocol for extracting pre-Rapid Expansion Protocol (REP) TILs • Assessed contaminating cells from the pre-REP process using multicolor flow cytometry • Evaluated the relationship between the immunological state of lymphocyte differentiation through REP and transduction efficiency Show less



Simcere Innovation

• Senior Research Associate I

  • Sep 2020 - May 2021

  Cambridge, Massachusetts, United States Biologics Discovery and Characterization • Utilized primary B cells isolated from immunized mice for novel antibody discovery • Isolated B cells from PBMCs using a MACS Tyto cell sorter • Characterized antibodies secreted from B cells for affinity and concentration • Developed methods for increasing throughput for novel antibody discovery • Delivered novel sequences for antibodies produced from single B cells through molecular cloning techniques • Transfected plasmids… Show more Biologics Discovery and Characterization • Utilized primary B cells isolated from immunized mice for novel antibody discovery • Isolated B cells from PBMCs using a MACS Tyto cell sorter • Characterized antibodies secreted from B cells for affinity and concentration • Developed methods for increasing throughput for novel antibody discovery • Delivered novel sequences for antibodies produced from single B cells through molecular cloning techniques • Transfected plasmids, harvested supernatants, and purified antigens using magnetic beads and FPLC Show less



Sana Biotechnology, Inc.

• United States
• Biotechnology Research
• 200 - 300 Employee

• Research Associate

  • Feb 2019 - Sep 2020

  Cambridge, Massachusetts, United States Protein Engineering • Developed methods for producing and screening lentiviral vectors in a high-throughput manner • Designed and tested plasmids for engineering cell-type specific targeting into lentiviral vectors while minimizing off-target effects • Analyzed specificity and function of antibody fragments via flow cytometry and biolayer interferometry (Octet) • Aided in implementing liquid handlers and developing protocols for using automation to increase throughput even… Show more Protein Engineering • Developed methods for producing and screening lentiviral vectors in a high-throughput manner • Designed and tested plasmids for engineering cell-type specific targeting into lentiviral vectors while minimizing off-target effects • Analyzed specificity and function of antibody fragments via flow cytometry and biolayer interferometry (Octet) • Aided in implementing liquid handlers and developing protocols for using automation to increase throughput even further • Developed transduction assays of primary stem and T cells • Analyzed quality of lentiviral production via assessment of functional titer, physical titer (GQA), and protein incorporation by automated western blot • Acted as primary flow manager for multiple flow cytometers (BD FACSCelesta and BD FACSFortessa), trained new employees on the instruments, and troubleshooted difficulties to ensure minimal disruption of use • Collaborated with other teams to transfer information and contribute to cross-functional projects to further improve therapeutic candidates • Routinely presented experimental results and conclusions in team and departmental research settings Show less



Cobalt Biomedicine, Inc.

• Biotechnology Research

• Research Associate

  • Dec 2016 - Feb 2019

  Cambridge, Massachusetts, United States • Isolated mitochondria from a variety of primary tissues to study respiration as a potential therapeutic • Helped developed in vivo gene therapy platform expanded upon by Sana Biotechnology • Sourced and screened plasmid constructs from the literature to test viability of the platform

• Research Associate

  • Aug 2016 - Dec 2016

  Cambridge, Massachusetts, United States