Meera Sathyadas
Research Associate I at Molecular Matrix, Inc.- Claim this Profile
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Bio
Experience
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Molecular Matrix, Inc.
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United States
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Medical Equipment Manufacturing
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1 - 100 Employee
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Research Associate I
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Feb 2022 - Present
● Perform research and development experiments under guidance of R&D supervisor ● Independent literature review, and experimental design and planning. ● Perform cell culture and tissue-engineering experiments ● Perform molecular biology experiments with standard techniques such as cloning, PCR, gel electrophoresis, SDS-PAGE, and gel staining. ● Competent in microbiology techniques to aid molecular cloning. ● Perform DNA/RNA extraction, miniprepping, transformation, restriction digest and cloning, Gibson assembly, and Gateway cloning. ● Mammalian cell transfection, and protein harvest. ● Perform diagnostic qPCR assays as needed. ● In accordance with SOPs, execute quality management protocols such as stability testing, reagent lot validation, and equipment calibration. ● Following CLIA regulations; maintain laboratory records and ensure appropriate and timely documentation of QC reports, temperature logs, calibration reports, etc. ● Maintain thorough records of all reagents and accurately track aliquot lots used in each assay. ● Assist lab in maintaining CLIA accreditation by performing proficiency testing and preparing for CDPH audits. ● Develop GLP documentation, and assist the lab in becoming and remaining GLP compliant
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Diamond Well Drilling Co
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United States
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Construction
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1 - 100 Employee
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Laboratory Manager
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Nov 2019 - Nov 2021
● Manage all aspects of a small laboratory; train new employees, manage budget and daily finances, track inventory and order supplies, and establish or maintain relationships with clients and vendors. ● Execute existing protocols and Quality Assurance/Quality Control System and implement new protocols as needed. ● Maintain QA/QC results, and Corrective/Preventative actions records in an organized and easily accessible manner. ● Maintain laboratory credentials through proficiency testing and annual ELAP audits. ● Keep thorough records of assays on forms and logs and in LIMs ● Manage records and results in LIMs; log samples and results, log personnel information and indicate access restrictions, maintain and differentiate credentialed and non-credentialed assays as well as outside contracted results, and generate results reports. ● Conduct microbiological tests on drinking water and wastewater samples such as SM9223B Presence/Absence, SM9223B Enumeration Quanti-tray, and SM9215B Heterotrophic Plate Count. ● Demonstrate rigorous aseptic technique when handling samples. ● Employ standard wet chemistry techniques in testing water for Ferrous Iron, Manganese, Total Hardness by titration, Total Dissolved Solids, Conductivity, and pH. ● Complete basic lab tasks such as preparing media, buffers, agar, autoclaving materials and waste, and washing glassware using lab detergents and acid baths. ● Communicate lab reports to clients and county health agencies
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CSP Labs
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United States
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Farming
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1 - 100 Employee
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PCR Technician
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Mar 2021 - Jun 2021
● Run assays such as multiplex qPCR, conventional PCR, DNA/RNA extraction, and gel electrophoresis. ● Perform high throughput, multiplex qPCR in a 384 well format. ● Analyze and interpret qPCR data, and prepare results reports. ● Properly track samples on paper forms and in LIMs. Create accurate and easy to follow maps of samples and assays when multiplexing. ● Operate various types of equipment such as multichannel pipettors, grinders, centrifuges, thermal cyclers, gel electrophoresis rigs, and laminar flow cabinets. ● Basic lab chores (make buffers, autoclave materials/trash, etc.) and general lab housekeeping (wash glassware, clean and tidy work area, etc.) ● Assist with lab safety (safety checks, training, etc.) ● Troubleshoot assay and equipment issues ● Maintain consumables inventory: track, order, unbox, and stock
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University of California, Berkeley
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United States
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Higher Education
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700 & Above Employee
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Student Intern
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Sep 2018 - May 2019
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National Aquarium
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Museums, Historical Sites, and Zoos
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200 - 300 Employee
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Student Volunteer
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2010 - 2014
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Education
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University of California, Berkeley
Bachelor's degree, Biology/Biological Sciences, General