Max Ezekiel

Associate Scientist II, AAV Process Development at Apertura Gene Therapy
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Location
Newton Centre, Massachusetts, United States, US
Languages
  • English Native or bilingual proficiency
  • Spanish Limited working proficiency

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Experience

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Associate Scientist II, AAV Process Development
      • Aug 2022 - Present
    • Philippines
    • Higher Education
    • 1 - 100 Employee
    • Graduate Student Researcher
      • Sep 2020 - Jun 2022

      - Designed and executed long-term experiments that culminated in a successfully defended thesis. - Engineered orthogonal synthetic receptors and tested how changing domains effects ligand-based response. - Collaborated with a team to design a roadmap for orthogonal receptor engineering in a work to be published. - Designed and executed long-term experiments that culminated in a successfully defended thesis. - Engineered orthogonal synthetic receptors and tested how changing domains effects ligand-based response. - Collaborated with a team to design a roadmap for orthogonal receptor engineering in a work to be published.

    • United States
    • Biotechnology Research
    • 200 - 300 Employee
    • Process Development Engineer II
      • Sep 2019 - Sep 2020

      - Developing upstream process for Lentivirus and AAV. - Transfering platform into a GMP environment. - Setting up both a process development and GMP lab space for capsid development and production. - Operation and Organization of instrumentation essential to running said process. - Developing upstream process for Lentivirus and AAV. - Transfering platform into a GMP environment. - Setting up both a process development and GMP lab space for capsid development and production. - Operation and Organization of instrumentation essential to running said process.

    • United States
    • Biotechnology
    • 1 - 100 Employee
    • AAV Process Development Engineer
      • Feb 2018 - Sep 2019

      Developed platform process for consistent, scalable production of multiple AAV serotypes from adherent and suspension HEK293 systems from a 40mL - 50L scale. - Developed process of growth and AAV production for the following reactor systems: Sartorius AMBR250, Eppendorf 10c, GE WAVE, Thermo 50L Sub. - Took a lead in upstream process development work in optimizing yield from a licensed cell line. - Wrote detailed SOPs/SBRs that led to successful production in CMOs. - Aided in development of quantification methods (qPCR, ELISA, Silver) and purification of multiple AAV serotypes. - As part of a 4-person team that has grown to 22 people, has aided in not only personnel acquisition and development, but also filling in gaps in an lean group. Show less

    • Events Services
    • 1 - 100 Employee
    • Associate Scientist
      • Aug 2016 - Feb 2018

      - Optimizing cell culture media for growth and production by manipulating its components and processes in a team lab environment. - Designing and executing experiments and presenting the results. - Instrumentation includes Mammalian bioreactors, shake flasks, AMBR system, High Performance Liquid Chromatography, Ultra Performance Liquid Chromatography, Amino Acid Analysis, Bio HT Analyzer, Nanodrop, and Packed Columns. - Contributed research for patent defense. - Optimizing cell culture media for growth and production by manipulating its components and processes in a team lab environment. - Designing and executing experiments and presenting the results. - Instrumentation includes Mammalian bioreactors, shake flasks, AMBR system, High Performance Liquid Chromatography, Ultra Performance Liquid Chromatography, Amino Acid Analysis, Bio HT Analyzer, Nanodrop, and Packed Columns. - Contributed research for patent defense.

    • Product Stewardship Intern
      • Jun 2014 - Aug 2014

      -Product Stewardship Intern. Worked on a consulting project for a large, confidential client working to reduce their footprint. -As part of a team, navigated through MSDS forms to determine the eligibility of over 20,000 chemicals. -Product Stewardship Intern. Worked on a consulting project for a large, confidential client working to reduce their footprint. -As part of a team, navigated through MSDS forms to determine the eligibility of over 20,000 chemicals.

    • United States
    • Software Development
    • 1 - 100 Employee
    • Software Intern
      • Jun 2012 - Aug 2012

      -Software developer in a start-up company working to navigate through phone trees for the client. -Responsible for dealing with customer call trees and programming results. -Software developer in a start-up company working to navigate through phone trees for the client. -Responsible for dealing with customer call trees and programming results.

    • United States
    • Hospitals and Health Care
    • 700 & Above Employee
    • Laboratory Assistant
      • Jun 2010 - Aug 2010

      -Research Lab Assistant. Worked in a lab researching cystic fibrosis through Pseudomonas in zebra fish. -Responsible for preparing various solutions and plates, aiding in procedural experimentations, and performing data analysis on Microsoft Office -Research Lab Assistant. Worked in a lab researching cystic fibrosis through Pseudomonas in zebra fish. -Responsible for preparing various solutions and plates, aiding in procedural experimentations, and performing data analysis on Microsoft Office

Education

  • Northwestern University
    Master of Science - MS, Chemical Engineering
    2020 - 2022
  • Tufts University
    Bachelor of Science - BS, Chemical Engineering; BS, Biotechnology
    2011 - 2015
  • Newton South High School
    High School
    2007 - 2011

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