Marlon de Gast

PHD Candidate at AMC Medical Research BV
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Contact Information
us****@****om
(386) 825-5501
Location
Amsterdam, North Holland, Netherlands, NL

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Experience

    • Research
    • 1 - 100 Employee
    • PHD Candidate
      • Sep 2018 - Present

      HIV vaccine HIV vaccine

    • Netherlands
    • Hospitals and Health Care
    • 700 & Above Employee
    • Master Thesis
      • Dec 2017 - Feb 2018

      Building on my affinity with microbiology and wanting to comprehend the underlying immunology behind vaccinations, I wrote my literature thesis about "Enhancing the immunogenicity of the HIV-1 Env-based SOSIP BG505.664 vaccine by adjuvants mimicking T cell help in a T cell-independent manner." Obtained grade: 8.5 Building on my affinity with microbiology and wanting to comprehend the underlying immunology behind vaccinations, I wrote my literature thesis about "Enhancing the immunogenicity of the HIV-1 Env-based SOSIP BG505.664 vaccine by adjuvants mimicking T cell help in a T cell-independent manner." Obtained grade: 8.5

    • Netherlands
    • Hospitals and Health Care
    • 700 & Above Employee
    • Master internship
      • Nov 2016 - Oct 2017

      During my final master internship on immunology, I worked on fine-tuning Factor H regulatory activity on host and bacterial surfaces. This was under the supervision of: Anna van Beek, Dr. D. Wouters, and Prof. dr. T.W. Kuijpers. Obtained grade: 8.0. Techniques used: ELISA (incl. cross-reactivity & complement binding assays), FACS, confocal microscopy, widefield microscopy, protein engineering, protein purifcation by HPLC, immunofluorescence, hemolytic assays, SDS-PAGE, Western Blot, gel electrophoresis, immunoprecipitation, and more. Show less

    • Netherlands
    • Hospitals and Health Care
    • 700 & Above Employee
    • Master internship
      • Dec 2015 - Sep 2016

      During my first master internship, I worked in the lab of Prof. dr. W. de Jonge under the supervision of Dr. R.M.J. van den Wijngaard and S. Botschuijver. Here, I worked intensively on Fungi induced macrophage training in patients with Irritable Bowel Syndrome (IBS). Obtained grade: 7.8 Techniques used: Cell culture, cell isolation, FACS, ELISA, qPCR, confocal microscopy, immunohistochemistry, HPLC, and more. During my first master internship, I worked in the lab of Prof. dr. W. de Jonge under the supervision of Dr. R.M.J. van den Wijngaard and S. Botschuijver. Here, I worked intensively on Fungi induced macrophage training in patients with Irritable Bowel Syndrome (IBS). Obtained grade: 7.8 Techniques used: Cell culture, cell isolation, FACS, ELISA, qPCR, confocal microscopy, immunohistochemistry, HPLC, and more.

    • Netherlands
    • Hospitals and Health Care
    • 700 & Above Employee
    • Bachelor internship
      • Feb 2015 - Jul 2015

      I worked in the lab of dr. Sander Ouburg and prof. dr. Servaas A. Morré, to study the effect of host genetic variations on the immune response of Chlamydia trachomatis infections: a pathway approach. Techniques used: RT-PCR, pathway analysis, ELISA, genotyping I worked in the lab of dr. Sander Ouburg and prof. dr. Servaas A. Morré, to study the effect of host genetic variations on the immune response of Chlamydia trachomatis infections: a pathway approach. Techniques used: RT-PCR, pathway analysis, ELISA, genotyping

    • Netherlands
    • Research Services
    • 700 & Above Employee
    • Literature Thesis
      • Jun 2013 - Jul 2013

      Literature thesis: Integration of metabolomic and transcriptomic data using Set Enrichment Analysis. Literature thesis: Integration of metabolomic and transcriptomic data using Set Enrichment Analysis.

Education

  • Universiteit van Amsterdam
    Master of Science - MS, Biomedical Sciences - Track Infection and Immunity.
    2015 - 2018
  • Universiteit van Amsterdam
    Bachelor of Science - BS, Average obtained grade 7.8 out of 10
    2012 - 2015

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