Markos Tsitsianopoulos

Research Assistant at BSRC Alexander Fleming
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Contact Information
us****@****om
(386) 825-5501
Location
GR
Languages
  • Greek Native or bilingual proficiency
  • English Professional working proficiency

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Bio

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Credentials

  • LAS Core Modules Course
    BSRC Alexander Fleming
    Jul, 2022
    - Nov, 2024
  • ICH Good Clinical Practice E6 (R2)
    The Global Health Network
    Mar, 2022
    - Nov, 2024
  • Bioconductor for Genomic Data Science
    Coursera
    Nov, 2020
    - Nov, 2024
  • Algorithms for DNA Sequencing
    Coursera
    Oct, 2020
    - Nov, 2024
  • Command Line Tools for Genomic Data Science
    Coursera
    Oct, 2020
    - Nov, 2024
  • Python for Genomic Data Science
    Coursera
    Oct, 2020
    - Nov, 2024
  • R Programming
    Coursera
    Oct, 2020
    - Nov, 2024
  • The Data Scientist's Toolbox
    Coursera
    Oct, 2020
    - Nov, 2024
  • Genomic Data Science with Galaxy
    Coursera
    Sep, 2020
    - Nov, 2024
  • Introduction to Genomic Technologies
    Coursera
    Sep, 2020
    - Nov, 2024
  • Programming for Everybody (Getting Started with Python)
    Coursera
    Sep, 2020
    - Nov, 2024
  • Certificate of Attendance - Current scientific trends and technological milestones in COVID-19 pandemic
    Σ.Ι.Γ.Ε. - ΣΥΝΔΕΣΜΟΣ ΙΑΤΡΙΚΩΝ ΓΕΝΕΤΙΣΤΩΝ ΕΛΛΑΔΑΣ
    Jan, 2020
    - Nov, 2024
  • Certificate of Attendance - 69th Congress of HSBMB
    Hellenic Society of Biochemistry and Molecular Biology - HSBMB
    Nov, 2018
    - Nov, 2024
  • Proficiency Certificate in English Language
    University of Michigan
    May, 2014
    - Nov, 2024

Experience

  • BSRC Alexander Fleming
    • Greece
    • Research Services
    • 1 - 100 Employee
    • Research Assistant
      • Nov 2022 - Present

      Project:Study of topologically associated gene co-regulation phenomena in transcriptomic data of cancer subtypes

    • Graduate Student Researcher
      • Oct 2021 - Nov 2022

      Project:Analysis of the spatial organization of gene expression in breast cancerSupervisor: Christoforos Nikolaou - Researcher B', Computational Genomics Group - Institute of Bioinnovation, Biomedical Sciences Research Center "Alexander Fleming"Methods practiced:• Programming (R programming language)• Identification of Domains of Co-ordinated Expression and analysis of their dynamics• RNASeq data mining and patient stratification analysis (TCGABiolinks)• Differential Expression Analysis (DESeq2)• Enrichment Analysis (Gene Ontology and Disease Ontology - clusterProfiler)• Genomic and statistical data visualization (ggplot2, plotgardener, pheatmap)• Data wrangling (Tidyverse)• Code optimization and debugging

  • University of Thessaly
    • Greece
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Student Researcher
      • Oct 2019 - Sep 2020

      Project: Characterization of long non-coding RNAs (lncRNAs) in gastric cancer, in correlation to copy number variations. Supervisor: Assistant Professor Antonis Giakountis, Molecular Biology and Genomics Lab, Department of Biochemistry and Biotechnology, UTH Techniques practiced: • DNA extraction (based on commercial and homemade kits) • RNA extraction (TRIzol method) • DNase treatment • cDNA synthesis (reverse transcription) • PCR • qPCR preparation and analysis • Gel electrophoresis • Transformation and amplification of vectors • shRNA oligo annealing and ligation in vectors • Transformation of ligation reaction products in bacterial cells • Screening for positive clones • Sequencing of vector shRNA constructs • Use of CPC2 and CPAT for the prediction of transcript coding potential • RNA-seq data analysis of differential gene expression between cultured and normal cells as well as between gastric cancer and normal biopsy samples

  • Hippocrates' General Hospital of Thessaloniki - Hellenic NHS
    • Hospitals and Health Care
    • 1 - 100 Employee
    • Student Internship
      • Jul 2019 - Aug 2019

      Techniques practiced: • Isolation of serum and plasma in blood and urine samples • Sample preparation for biomarker analysis Techniques practiced: • Isolation of serum and plasma in blood and urine samples • Sample preparation for biomarker analysis

Education

  • Aristotle University of Thessaloniki (AUTH)
    Master's degree, Stem Cells and Regenerative Medicine
    2020 - 2022
  • University of Thessaly
    Bachelor's degree, Biochemistry and Biotechnology
    2016 - 2020
  • 1st High School of Thessaloniki
    2014 - 2016

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