Experience

STEPUPIORS

• Serbia
• Public Health
• 1 - 100 Employee

• Research Professor

  • Oct 2022 - Present



UCL

• United Kingdom
• Research Services
• 700 & Above Employee

• Associate Fellow

  • Feb 2009 - Dec 2011

  o The effect of dichloroacetate on proteomes of malignant and normal cells. Dichloroacetate restores the oxidative phosphorylation metabolism which is often hampered in malignant cells. The effect of this treatment on a cellular proteome is investigated by use of quantitative proteomics. o Characterization of the differential effect of Cdc7 silencing on survival of malignant and normal cells. Subcellular quantitative proteomics was applied to examine the mechanism of Cdc7 siRNA-induced silencing, which leads to death of malignant cells but not normal cells. o The role of cell cycle proteins in the genetic predisposition to the coronary artery disease. The risk of heart disease was previously associated with single nucleotide polymorphisms (SNPs) on the 9p21.3 allele in the vicinity of CDKN2a and CDKN2b tumour suppressor genes. We identified the functional single nucleotide polymorphism (SNP) on the 9p21 allele (rs10738609) for the first time. Molecular mechanism of the rs10738609-mediated cardiovascular disease risk through the disruption of CDK – inhibitor proteins CDKN2a and CDKN2b is characterized by genetic and proteomics approaches. - Characterization of mechanisms responsible for the genetic predisposition to the coronary artery disease. Identified for the first time the functional single nucleotide polymorphism (SNP) on the 9p21 allele (rs10738609). It was revealed that rs10738609 may exert an increased risk of coronary artery disease by means of a long-range transcriptional control of nearest genes (about 100kb downstream) through disruption of a distant cis-acting genomic element. Show less



Imperial College London

• Research Services
• 700 & Above Employee

• Career Development Fellow

  • Feb 2006 - Feb 2009

  Identification of the novel interacting partners of cyclin dependent kinase subunit (Cks) proteins. Discovered a novel Cdk-dependent interaction between Cks protein and the mitochondrial single-stranded DNA-binding protein (mtSSB) by use of affinity purification combined with mass spectrometry. mtSSB is known to participate in replication of mitochodondrial DNA. It binds both Cks1 and Cks2 and undergoes Cdk-dependent phosphorylation. It was demonstrated that mitochondrial morphology and DNA integrity are compromised in cells depleted of both Cks proteins or with inhibited Cdk activity. This work reveals a novel direct role of cell cycle proteins in controlling mitochondrial DNA replication. (Molec. Cell. Proteomics 2010, 9:145). Show less



UCL

• United Kingdom
• Research Services
• 700 & Above Employee

• Principal Research Fellow

  • Jun 2003 - Jun 2006

  Identification of the function of grancalcin. Identified for the first time the function of grancalcin, a protein specifically expressed in neutrophils. Grancalcin has a role in neutrophil adhesion (Cell. Immunol. 2006, 240:116). Subproteome analysis of the neutrophil cytoskeleton. Analyzed cytoskeletal in human neutrophils: cytosolic skeleton, plasma membrane skeleton and phagosome membrane skeleton. Using a proteomic approach, 138 nonredundant proteins were identified. A number of novel interactions between cytoskeleton and metabolic enzymes were revealed. On the basis of obtained results it was concluded that macromolecular organization and localized enrichment of metabolic enzymes may be important for their function (Proteomics, 2009, 9:2037). Show less



Max Planck Institute for Experimental Medicine

• Postdoctoral Research Fellow

  • Jul 1996 - May 2003

  Identification of the molecular mechanism(s) underlying the anti-apoptotic effect of the corticotropin-releasing factor (CRF). Discovered an anti-apoptotic effect and potential neuroprotective action of CRF (J. Neurochem. 2003, 84:1074). Characterization of the molecular mechanisms of immunoregulation by CRF. Identified immune cells expressing CRFR1 receptors were for the first time. CRF modulates cytokine production by neutrophils (J. Neuroimmunol. 2000, 103:153; J. Immunol. 1999, 162:3013). Show less



fdf

• df

  • 1900 - 2000