Mark Margosian

Cyclotron Engineer at Siemens
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Greater Minneapolis-St. Paul Area

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Cayla Ruch, PhD

Mark is a detail-oriented worker who manages his time wisely and is able to complete tasks in an accurate manor. Mark is able to offer creative solutions when problems arise and is able to teach others how to efficiently and effectively use laboratory equipment.

Cary Mariash

Mark was a laboratory technician in my laboratory while I was on the faculty at the University of Minnesota. He performed his duties extremely well. He was conscientious, hard working, worked independently, and carefully. When he left for a better position, I was sorry to see him go.

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Experience

    • Germany
    • Automation Machinery Manufacturing
    • 700 & Above Employee
    • Cyclotron Engineer
      • Jun 2017 - Present

      Perform PM and CM's on Cyclotron Perform PM and CM's on Cyclotron

    • United States
    • Hospitals and Health Care
    • 700 & Above Employee
    • Clinical Engineering Technician
      • Jan 2013 - Jun 2017

      * Identify, analyze and troubleshoot instruments for corrective maintenance repair; including maintenance on robotic analyzers, such as: Sakura TissueTek X50 sample processor Polymedco OC-Auto Micro 80 Analyzer Certified to work on Thermo Fisher Scientific Phadia 250 analyzer * Field service support for 20 clinic laboratories. * Develop procedures and perform preventive maintenance in compliance with CAP, AABB and NFPA standards. * Responsible for all laboratory, and 3 general medicine departments. * Establish a working customer relationship to develop a laboratory biomedical equipment program for the Park Nicollet Health System. * On-call status for main hospital, clinics and laboratory. * Computer knowledge in windows environments and interfacing systems and principles. Show less

    • Assistaint Scientist
      • May 2000 - Jan 2013

      * Support equipment through assembly, installation, calibration, repair, and in-service training. Indirect calorimetry, wheel running, treadmill running, sleep/wake telemetry, IR physical activity, automated feeding, MRI body composition. * Develop and perform laboratory assays such as: Affymetrix microarray procesing Protein, DNA, and RNA extraction and quantification Real-Time reverse transcriptase PCR Indirect calorimetry GCMS Capillary electrophoresis, DNA Sequencing , Genotyping. Northern, Southern and westerns blot Hybridization Radio Immuno Assay (RIA) Cell culture and aseptic technique Radioisotope infusion studies Spectrophotometric assays Thin layer chromatography (TLC) Intra-venous catheter implantation * Develop, execute, and troubleshoot procedures for the operation of equipment, such as: ABI - Prism 310 Genetic Analyzer Agilent - 2100 Bioanalyzer BioRad - iCycler Beckman Coulter - DU 800 UV/Vis Spectrophotometer Beckman Coulter - Optima L-80 Ultracentrifuge Hewlet Packard - 5890 Gas Chromatography / Mass Spectrometry MJ Research PTC - 200 Thermo Cyclers Molecular Devices - Microplate Reader Nuaire - CO2 chamber and Laminar Flow Hood Packard - Scintillation and Gamma Counters Roche - Light Cycler 1.5 Thermo Scientific - NanoDrop 8000 Sorvall - Desktop Centrifuges Savant - SpeedVac Concentrators Molecular Dynamics - Storm 860 phosphorimager / fluorimager * Supervise and train personnel. Write SOP’s, and portions of documents. * Computer support through hardware, software and firmware installation, troubleshooting. Show less

    • 1 - 100 Employee
    • Assistant Scientist
      • Mar 2000 - Jan 2013

      PUBLICATIONS: Authorship 1. Donnelly KL, Margosian MR, Sheth SS, Lusis AJ, and EJ Parks, 2004. “Increased Lipogenesis and Fatty Acid Re-esterification Contribute to Hepatic Triacylglycerol Stores in Hyperlipidemic Txnip -/- Mice”. 2. Zhu Q, Mariash A, Margosian MR, Gopinath S, Fareed MT, Anderson GW, and CN Mariash, 2001. “Spot 14 gene Deletion increases hepatic de novo lipogenesis” Endocrinology, 142:4363-4370. Acknowledgments 1.Mark G. LeSage, Jennifer L. Perry, Catherine M. Kotz, David Shelley, William A. Corrigall, “Nicotine Self- administration in the rat: effects of hypocretin antagonists and changes in hypocretin mRNA” Psychopharmacology, 2010; 209: 203-212 2.Cydne A. Parry, Marco Pravetoni, Jennifer A. Teske, Carolina Aguado, Darin J. Erickson, Juan F. Medrano, Rafael Lujan, Catherine M. Kotz, Kevin Wickman, “Predisposition to Late-onset Obesity in GIRK4 Knockout Mice” PNAS, 2008; 105, no.23, R8153 3.ChuanFeng Wang, Eric Bomberg, Charles Billington, Allen Levine, and Catherine M. Kotz, “Brain- derived neurotrophic factor in the hypothalamic paraventricular nucleus reduces energy intake” Am J Physiol Regul Integr Comp Physiol, 2007; 293: R1003-R1012. 4. ChuanFeng Wang, Eric Bomberg, Charles Billington, Allen Levine, Catherine M. Kotz, “Brain-derived neurotrophic factor in the hypothalamic paraventricular nucleus increases energy expenditure by elevating metabolic rate” Am J Physiol Regul Integr Comp Physiol, 2007; 293: R992-R1002. 5. J.A. Teske, A.S. Levine, M. Kuskowski, J.A. Levine, C.M. Kotz, “Elevated hypothalamic orexin signaling, sensitivity to orexin A, and spontaneous physical activity in obesity-resistant rats” Am J Physiol Regul Integr Comp Physiol, 2006; 291: R889–R899. 6. Qihong Zhu, Grant W. Anderson, Gregory T. Mucha, Elizabeth J. Parks, Jennifer K. Metkowski and Cary N. Mariash, “The Spot 14 Protein Is Required for de Novo Lipid Synthesis in the Lactating Mammary Gland” Endocrinology, August 2005; 146(8): 3343-3350. Show less

    • United States
    • Medical Practices
    • 1 - 100 Employee
    • Research Associate
      • 2003 - 2004

      rtPCR and Affymetrix Microarray processing. rtPCR and Affymetrix Microarray processing.

    • Recording Artist ( Colfax Abbey)
      • 1993 - May 2000

      1996-present : Digital Audio Workstation Engineer: I started DAW recording during it's inception years, building systems and integrated / tested hundreds of software programs. 1994-1999 : Recording artist (Colfax Abbey) on Twin Tone records, as guitarist. 1996-present : Digital Audio Workstation Engineer: I started DAW recording during it's inception years, building systems and integrated / tested hundreds of software programs. 1994-1999 : Recording artist (Colfax Abbey) on Twin Tone records, as guitarist.

    • United States
    • Non-profit Organizations
    • 1 - 100 Employee
    • Home Health Aide
      • 1994 - 1999

      Home health aide for mentally and physically challenged adults. Home health aide for mentally and physically challenged adults.

Education

  • Dakota County Technical Colledge (A.A.S BIOMEDICAL EQUIPMENT)
    A.A.S., Biomedical Equipment Technology (BMET)
    2010 - 2012
  • University of Minnesota-Twin Cities (B.S. BIOLOGY)
    B.S., Biology
    1994 - 2000

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