Experience

Pure Biologics

• Poland
• Biotechnology Research
• 1 - 100 Employee

• Research Team Coordinator

  • Jun 2019 - Present

• Research And Development Specialist

  • Mar 2019 - Jun 2019



Wroclaw University of Science and Technology

• Poland
• Higher Education
• 700 & Above Employee

• PHD Student

  • Jun 2013 - Nov 2022

  Obtaining IgY antibodies specific to Tumor Associated NADH Oxidase (tNOX) and their biochemical evaluation: -epitope selection for hens immunization and antigen preparation (peptide synthesis and conjugation with carrier proteins) -IgY antibodies isolation using precipitation method -evaluation of polyclonal antibodies by ELISA and western blot (detection limit, titer, time response, avidity, cross reactivity) -evaluation of polyclonal antibodies by flow cytometry, IHC and IF -labeling antibodies (gold, biotin, fluorescein, enzyme) Application of novel Phosphonic-Type Activity-Based Probes for Neutrophil Serine Proteases in Spleen Lysates of Different Organisms Synthesis of fluorogenic substrates, inhibitors, and molecular probes specific for human neutrophil proteinase NSP4 and its evolutionary analogues: -determination of Activity-Based Probes specificity by western blot Show less



University of Chicago

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Jul 2017 - Dec 2018

  Research area: Developing high-affinity binders for different target proteins via Phage Display Selection Bispecific antibody generation using plug-and-play system -target proteins expression and purification from mammalian cell cultures (suspension cell culture) -generation of high-affinity binders for different targets via Phage Display Selection -expression and purification of proteins (eg. Fab) from bacterial cell culture using ÄKTA protein purification system -evaluation of developed binders by phage-ELISA, multipoint ELISA, surface plasmon resonance (SPR) -epitope binning experiments -cell binding assays (transfected adherent cells cultures/PBMC) flow cytometry -procuring high affinity bispecific binders by plug-and-play approach -investigation of functional basis of generated bispecifics – determination if the created proteins are able to bind and activate T cells (mammalian cell culture, cell assays) Show less



The University of Texas Health Science Center at Houston (UTHealth Houston)

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Graduate Student Internship

  • Mar 2017 - May 2017

  Study of the interaction of peripheral immune cells with the brain and their role in the progression of Parkinson’s disease: -performing ELISA alpha-synuclein peptide-based assay to determination of monocyte activity in the serum of PD patients -developing a method for high-throughput monocyte enrichment using magnetic bead based assay -performing flow cytometry -immunohistochemistry staining - sectioning and staining paraffin embedded biopsy sections Study of the interaction of peripheral immune cells with the brain and their role in the progression of Parkinson’s disease: -performing ELISA alpha-synuclein peptide-based assay to determination of monocyte activity in the serum of PD patients -developing a method for high-throughput monocyte enrichment using magnetic bead based assay -performing flow cytometry -immunohistochemistry staining - sectioning and staining paraffin embedded biopsy sections



Jagiellonian University

• Poland
• Research Services
• 700 & Above Employee

• Graduate Student Internship

  • May 2016 - May 2016

  Expression and purification of NS2B-NS3 protease form Zika virus Expression and purification of NS2B-NS3 protease form Zika virus



DTU - Technical University of Denmark

• Denmark
• Research Services
• 700 & Above Employee

• Graduate Student Internship

  • Jul 2015 - Jul 2015

  Phenotyping γδ T lymphocytes of various origins using flow cytometry Phenotyping γδ T lymphocytes of various origins using flow cytometry



Wroclaw University of Science and Technology

• Poland
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Jun 2012 - Sep 2012

  Research area: IgY antibodies isolation from egg yolk, purification and biochemical evaluation (WB, ELISA, IP) Research area: IgY antibodies isolation from egg yolk, purification and biochemical evaluation (WB, ELISA, IP)



Grupa HASCO

• Poland
• Pharmaceutical Manufacturing
• 200 - 300 Employee

• Student Internship

  • Aug 2010 - Aug 2010