Experience

Friedrich Miescher Laboratory of the Max Planck Society

• Germany
• Research Services
• 1 - 100 Employee

• Senior Staff Scientist

  • Aug 2015 - Present

  Laboratory management and molecular biology methods, next generation sequencing (TruSeq and Nextera/Tn5 library preparation). Tn5 transposase over-expression and tagmentation optimization for pg-ng imput genomic DNA samples. Development and implementation of novel sequencing (on-magnetic-bead tagmentation, Tn5 long-read sequencing) and other molecular biology methods. Two novel molecular biology methods developed: a.) Haplotagging : linked-read sequencing using bead-bound Tn5 transposase and 85 million barcodes b.) Novel high throughput DNA size selection method for >10kb DNA molecules Show less



The National Institutes of Health

• United States
• Biotechnology Research
• 700 & Above Employee

• Research Scientist

  • Nov 2007 - Nov 2013

  Research in Endocrinology, aimed at the investigation of the cellular signaling, gene expression and hormone secretion in anterior pituitary cells. • Routinely performed isolation, optimization and maintenance of rat and mouse pituitary primary cell-cultures and maintained various cancer cell lines in the laboratory; • Performed radioimmunoassays (RIA) and enzyme-linked immunosorbent assays (ELISA) of secreted pituitary hormones and cAMP/cGMP nucleotides from perifused and cultured pituitary cells in normal and pharmacologically challenged conditions; • Extensively analyzed RNA transcriptome and proteome of GnRH-controlled gene network of rat pituitary gonadotrophs using Next Generation Sequencing (RNA-Seq), TaqMan qRT-PCR analysis and Western Blotting; • Recombinantly expressed and purified tagged and fluorescently labeled fusion proteins, validated commercially available antibodies; • Performed and evaluated siRNA efficacy of gene silencing primarily by qRT-PCR and Western Blotting; • Conducted standard laboratory techniques such as DNA/RNA isolation, DNA gel electrophoresis, restriction analysis, plasmid preparation and extraction, standard and dose response curve generation, maintenance of laboratory stocks and preparation of reagents; • Supervised and trained new staff and mentored summer students. Show less



Institute of Physiology, the Czech Academy of Sciences

• Czechia
• Research Services
• 1 - 100 Employee

• PhD research

  • 2002 - 2007

  Research focused on the identification and the characterization of steroid metabolites and steroid metabolizing enzymes in chicken and rat intestine and other tissues. • Solid-state extraction and HPLC/Mass spectrometry of steroid hormones and their metabolites from various rat and chicken tissues with emphasis on the activity of glucocorticoid metabolizing enzymes (11β-HSD1 and 2); • Identification, bacterial recombinant expression and affinity column purification of a novel chicken 20β-hydroxy-steroid dehydrogenase enzyme (20β-HSD); • Gene expression analysis, RNA extraction, plasmid preparation, transfection and extraction; • Performed enzyme activity assays on native and recombinant steroid-metabolizing enzymes, identified their steroid ligands and metabolites and evaluated the effect of these modifications on steroid receptor binding; • Assisted with writing of grants and publications. Show less



Scottish Fish Immunology Research Centre, University of Aberdeen

• Aberdeen, Great Britain

• Student Exchange Researcher

  • Sep 2004 - Feb 2005

  Focused on the basics of flow cytometry and quantitative Real-time PCR. Hands on experience working with fish (Rainbow Trout), isolation of fish blood leukocytes and Real-Time PCR analyses of cytokine gene expression. Focused on the basics of flow cytometry and quantitative Real-time PCR. Hands on experience working with fish (Rainbow Trout), isolation of fish blood leukocytes and Real-Time PCR analyses of cytokine gene expression.



Univerzita Komenského v Bratislave

• Slovakia
• Higher Education
• 700 & Above Employee

• MSc. Biology student

  • 1997 - 2002

  • Performed in vitro perfusion of rat heart, recorded epicardial electrograms, induction of heart fibrillation, ischemia and re-perfusion injury in diabetic rats; • Prepared cryostat sections of heart tissue for immunolabeling of gap-junction remodeling after fibrillation and ischemia. • Performed in vitro perfusion of rat heart, recorded epicardial electrograms, induction of heart fibrillation, ischemia and re-perfusion injury in diabetic rats; • Prepared cryostat sections of heart tissue for immunolabeling of gap-junction remodeling after fibrillation and ischemia.