Experience

Universität Greifswald

• PhD student

  • Jan 2020 - Present



Enzymicals AG

• Trainee

  • Nov 2019 - Dec 2019



Pasticceria Marisa srl

• Shop Assistant (Banconiere)

  • Feb 2015 - Oct 2019

  This occupation allowed me to keep my university studies and have a minimum economic independence; although temporary, because I am looking for a job more relevant to my studies, it has taught me the teamwork, the determination to pursue one's own projects, attention to details, the ability to work in different conditions (especially under stress) and the constructive comparison both with colleagues and owners but also with customers.ITALIANOQuesta occupazione mi ha permesso di… This occupation allowed me to keep my university studies and have a minimum economic independence; although temporary, because I am looking for a job more relevant to my studies, it has taught me the teamwork, the determination to pursue one's own projects, attention to details, the ability to work in different conditions (especially under stress) and the constructive comparison both with colleagues and owners but also with customers.ITALIANOQuesta occupazione mi ha permesso di mantenere gli studi universitari e avere una minima indipendenza economica; sebbene temporaneo, poiché ricerco un lavoro più attinente ai miei studi, è un impiego che mi ha insegnato il lavoro di squadra, la determinazione nel perseguire i propri progetti, l'attenzione ai dettagli, la capacità di lavorare in diverse condizioni (soprattutto sotto stress) e il confronto costruttivo sia con colleghi e titolari ma anche con la clientela. Show more Show less



Università degli Studi di Padova

• Trainee

  • May 2017 - Jul 2018

  I carried out the master's thesis "Selection and characterization of variants of a bacterial formate dehydrogenase obtained by directed evolution" (in English) in the laboratory of Molecular Biology and Biocatalysis of Prof. Elisabetta Bergantino at the Department of Biology of the University of Padua.During the training, I was involved in the expression and characterization of variants of a bacterial formate dehydrogenase previously obtained by directed evolution. In this way, I was able… I carried out the master's thesis "Selection and characterization of variants of a bacterial formate dehydrogenase obtained by directed evolution" (in English) in the laboratory of Molecular Biology and Biocatalysis of Prof. Elisabetta Bergantino at the Department of Biology of the University of Padua.During the training, I was involved in the expression and characterization of variants of a bacterial formate dehydrogenase previously obtained by directed evolution. In this way, I was able to gain experience in the preparation of PCR, SDS-PAGE and Western-Blot reactions, in the cloning of gene inserts in plasmid vectors, in the use of E. coli as an expression system (fromtransformation and selection of the transformed up to cell culture and induction of the expression) and in the expression of recombinant proteins and their purification by affinity column (IMAC).ITALIANOHo svolto la tesi magistrale "Selection and characterization of variants of a bacterial formate dehydrogenase obtained by directed evolution" (in inglese) nel laboratorio di Biologia Molecolare e Biocatalisi della prof.ssa Elisabetta Bergantino presso il Dipartimento di Biologia dell'Università degli studi di Padova.Durante il tirocinio mi sono occupato dell'espressione e caratterizzazione di varianti di una formiato deidrogenasi batterica precedentemente ottenute tramite evoluzione diretta. In questo modo ho avuto modo di acquisire esperienza nell'allestire reazioni di PCR, SDS-PAGE e Western-Blot, nel clonaggio di inserti genici in vettori plasmidici, nell'uso di E. coli come sistema di espressione (dallatrasformazione e selezione dei trasformati fino alla coltura di cellule e all'induzione dell'espressione) e nell'espressione di proteine ricombinanti e loro purificazione mediante colonna di affinità (IMAC). Show more Show less



Università degli Studi di Padova

• Trainee

  • Jun 2015 - Sep 2015

  I carried out the bachelor's thesis "Evaluation of cytotoxic effects of free and encapsulated gemcitabinein nanoparticles on human lymphocytes" in the laboratory of Prof. Susanna Mandruzzato at the Veneto Oncological Institute (IOV) - IRCCS of Padua.During the training, I compared free gemcitabine with that encapsulated in nanoparticles, to determine if the latter was able to decrease the cytotoxic effect of the drug on human lymphocytes. I have gained experience using the MTT… I carried out the bachelor's thesis "Evaluation of cytotoxic effects of free and encapsulated gemcitabinein nanoparticles on human lymphocytes" in the laboratory of Prof. Susanna Mandruzzato at the Veneto Oncological Institute (IOV) - IRCCS of Padua.During the training, I compared free gemcitabine with that encapsulated in nanoparticles, to determine if the latter was able to decrease the cytotoxic effect of the drug on human lymphocytes. I have gained experience using the MTT colourimetric assay, cultivating tumour cells (fibroblasts) and working in sterility under the biological hood.ITALIANOHo svolto la tesi triennale "Valutazione di effetti citotossici di gemcitabina libera e incapsulatain nanoparticelle su linfociti umani" nel laboratorio della prof.ssa Susanna Mandruzzato presso l'Istituto Oncologico Veneto (IOV) - IRCCS di Padova.Durante il tirocinio ho confrontato la gemcitabina libera con quella incapsulata in nanoparticelle, per determinare se quest'ultima fosse in grado di diminuire l'effetto citotossico del farmaco su linfociti umani. Ho fatto esperienza utilizzando il saggio colorimetrico MTT, coltivando cellule tumorali (fibroblasti) e lavorando in sterilità sotto cappa biologica. Show more Show less