Experience

Harvard Kenneth C. Griffin Graduate School of Arts and Sciences

• United States
• Higher Education
• 100 - 200 Employee

• Graduate Research Assistant

  • Aug 2022 - Present

  Brian Liau Research Group. Brian Liau Research Group.



Pairwise

• United States
• Food and Beverage Manufacturing
• 100 - 200 Employee

• Protein Science Summer Internship

  • Jun 2022 - Aug 2022

  Assisted with the development and optimization of novel genome editing technologies for use in the food and agricultural industries. Assisted with the development and optimization of novel genome editing technologies for use in the food and agricultural industries.



OliX Pharmaceuticals, Inc.

• South Korea
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• Oligonucleotide Synthesis/Purification Laboratory Assistant

  • Mar 2022 - Jun 2022

  Prepared buffers and reagents for oligonucleotide processing. Used UV-Vis Spectrometry, HPLC, and LCMS to purify and analyze oligonucleotides. Assisted with the development of standard operating procedures and formal processes related to pipette calibration. Prepared buffers and reagents for oligonucleotide processing. Used UV-Vis Spectrometry, HPLC, and LCMS to purify and analyze oligonucleotides. Assisted with the development of standard operating procedures and formal processes related to pipette calibration.



UC San Diego

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Research Assistant

  • Jul 2020 - Jun 2022

  Komor Research Group Faculty Advisors: Dr. Alexis Komor & Dr. Francesco Paesani Ph.D. Student Mentors: Carlos Vasquez & Kartik Lakshmi My research is focused on characterizing point mutations in a DNA repair gene that are inherited by patients with an increased risk of developing colorectal cancer. To investigate the effect of individual mutations on DNA repair, I used a genome-editing technique called base editing to artificially introduce each point mutation into the genome of mammalian cells. After the desired modifications were verified via genome sequencing, the DNA repair outcomes inside individual cells were assessed using a fluorescence-based assay, flow cytometry, and my R script. Following these experiments, we realized that a previously unclassified point mutation should be designated as pathogenic because its DNA repair efficiency was reduced to compared to wild-type. Motivated to discover its biochemical mechanism, we employed AMBER molecular dynamics simulations and co-immunoprecipitation to probe changes in the mutant DNA repair protein's structure that may affect its interactions with its damaged DNA substrate and protein partners. Moreover, we used western blot and reverse transcriptase quantitative polymerase chain reaction to investigate changes in relative gene expression in the edited cells. Show less



CRC Oncology

• United States
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• Intern

  • Feb 2020 - Mar 2020

  Transcribed regulatory standard operating procedures for cancer drugs into the company’s format. Transcribed regulatory standard operating procedures for cancer drugs into the company’s format.



Simple Learning Academy

• United States
• Education Administration Programs

• Private Tutor

  • Oct 2018 - Jun 2019

  Initiated a tutoring organization to support local students taking chemistry and mathematics courses in middle and high school. Created a website linking students with qualified tutors. Over fifteen students benefited from these services and received eight testimonials for outstanding service. Initiated a tutoring organization to support local students taking chemistry and mathematics courses in middle and high school. Created a website linking students with qualified tutors. Over fifteen students benefited from these services and received eight testimonials for outstanding service.



Del Taco

• United States
• Restaurants
• 700 & Above Employee

• Line Cook

  • Aug 2018 - Oct 2018

  My role included working at the grill, taco bar, preparation, and cashier stations, in addition to cleaning. My role included working at the grill, taco bar, preparation, and cashier stations, in addition to cleaning.