Experience

SARomics Biostructures AB

• Sweden
• Biotechnology Research
• 1 - 100 Employee

• Research Scientist in Protein Science

  • Jun 2023 - Present

  ✔ Production and characterization of medically and industrially relevant proteins, including DNA-binding proteins, kinases, antibody-antigen complexes, and a wide variety of therapeutic targets.✔ Protein expression in E. coli and insect cells using the baculovirus expression vector system.✔ Protein isolation and purification by chromatographic methods (affinity, ion exchange, size exclusion, hydrophobic interaction) as well as ultracentrifugation, filtration (including TFF), and dialysis. ✔ Protein analysis and characterization by SDS-PAGE, UV-Vis spectroscopy, Western blotting, Analytical SEC, DLS, thermal shift assays, and high-resolution liquid chromatography / mass spectrometry (LC/MS QTOF).✔ High-throughput crystallization of proteins using commercial and custom screens for X-ray diffraction experiments.✔ Molecular cloning, bacterial transformation, insect cell transfection.✔ SCRUM framework. ✔ Documentation, analysis, and reporting of experimental data.✔ Communication and interaction with a wide variety of clients, including pharmaceutical companies and universities from the US, Europe, Asia and Middle East. Show less

• Associate Research Scientist in Protein Science

  • Sep 2022 - Jun 2023



Lund University

• Sweden
• Higher Education
• 700 & Above Employee

• Doctoral Researcher in Biology and Physical Chemistry

  • Nov 2017 - Sep 2022

  Reconstitution of membrane-bound enzymes into artificial lipid bilayers for structural studies using biophysical and neutron scattering techniques. ✔ Molecular cloning and expression of recombinant proteins in Escherichia coli and in insect cells using the baculovirus expression vector system (BEVS). ✔ Purification of proteins using liquid chromatography systems. ✔ Extraction, purification and analysis of lipids from microbial cultures. ✔ Analysis of proteins and protein-lipid interactions using biophysical methods: UV-Vis absorbance, dynamic and static light scattering (DLS, SLS), differential scanning fluorimetry (DSF), quartz cristal microbalance with dissipation monitoring (QCM-D), and Fourier Transform Infrared (FTIR) spectroscopy. ✔ Formulation, purification and analysis of lipid particles (liposomes, micelles, lipid bilayers). ✔ Study of protein-lipid interactions using neutron scattering techniques such as reflectometry and small angle scattering. ✔ Reporting and documentation of lab results. ✔ Academic writing and research dissemination in international conferences and peer-reviewed journals. ✔ Supervision of bachelor-level and master-level students. Show less



Helmholtz Centre for Infection Research

• Germany
• Research Services
• 200 - 300 Employee

• Researcher in Chemical Biology

  • Apr 2016 - Oct 2017

  ✔ Mode of action studies of antibacterial and antiproliferative compounds using chemical biology and bioinformatic approaches, including next-generation DNA and RNA sequencing. ✔ Cultivation of mammalian cell lines for cytotoxicity studies and MIC studies with bacterial and yeast pathogens. ✔ Extraction and purification of secondary metabolites and analysis of small molecules using HPLC, GC-MS and LC-MS. ✔ Mode of action studies of antibacterial and antiproliferative compounds using chemical biology and bioinformatic approaches, including next-generation DNA and RNA sequencing. ✔ Cultivation of mammalian cell lines for cytotoxicity studies and MIC studies with bacterial and yeast pathogens. ✔ Extraction and purification of secondary metabolites and analysis of small molecules using HPLC, GC-MS and LC-MS.



Arkema

• France
• Chemical Manufacturing
• 700 & Above Employee

• Project Manager

  • Jul 2011 - Jul 2013

  ✔ Design and implementation of a wastewater treatment system based on chemical flocculation and bioremediation using GMP guidelines. ✔ In charge of a team of 5 technicians. ✔ Design and implementation of a wastewater treatment system based on chemical flocculation and bioremediation using GMP guidelines. ✔ In charge of a team of 5 technicians.



Tecnológico de Monterrey

• Mexico
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Aug 2009 - May 2010

  ✔ Design and operation of a pilot-scale bioethanol plant using sweet sorghum as the raw material. ✔ Design and operation of a pilot-scale bioethanol plant using sweet sorghum as the raw material.



IPICyT

• Mexico
• Research Services
• 200 - 300 Employee

• Research Assistant

  • Jun 2008 - Jul 2008

  ✔ Molecular cloning, bacterial genetic modification, agarose gel electrophoresis, SDS-PAGE, Western blotting. ✔ Molecular cloning, bacterial genetic modification, agarose gel electrophoresis, SDS-PAGE, Western blotting.