Experience

Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences

• Poland
• Chemical Manufacturing
• 1 - 100 Employee

• Professor

  • Oct 2002 - Present

  Research topics: - Investigation of enzymatic substrates spectrum and analysis of reaction products with LC-MS and GC-MS methods. - The study of kinetics and thermodynamics of enzyme's reduction and oxidation half-cycles (temperature kinetic measurements and isothermal titration calorimetry). - Enzyme inhibition and deactivation study. - The quantum chemical modeling of reaction pathways (especially ethylbenzene dehydrogenase) - Homology modeling of enzyme… Show more Research topics: - Investigation of enzymatic substrates spectrum and analysis of reaction products with LC-MS and GC-MS methods. - The study of kinetics and thermodynamics of enzyme's reduction and oxidation half-cycles (temperature kinetic measurements and isothermal titration calorimetry). - Enzyme inhibition and deactivation study. - The quantum chemical modeling of reaction pathways (especially ethylbenzene dehydrogenase) - Homology modeling of enzyme structures.< - Modeling of quantitative structure activity relationships (QSAR) of catalytic activity with Multiple Linear Regression (MLR) and Artificial Neural Network (ANN) methods. Methodology: - Investigation of enzyme activities towards different substrates basing on spectroscopic activity assay. Rapid mixing stopped flow kinetic measurements of half-cycle redox reactions. - FPLC and electrophoresis techniques for the enzyme purification. - LC-MS and GC-MS for separation of reaction's products. - Isothermal titration calorimetry for investigation of thermodynamics of enzyme reaction. - Quantum chemical modeling of the reaction pathway with cluster model of molybdenum active site as well as with QM:MM modeling of the whole enzyme subunit. Verification of the hypothetical reaction mechanism with modeling of the reaction with various substrates. - Prediction of biological activity with ANN and QSAR equations based on quantum chemical parameters.

• Deputy Director for Research

  • Jul 2018 - Present

  Kraków



Joint Laboratory of Biotechnology and Enzyme Catalysis, ICSC PAS

• head of the lab

  • Jun 2008 - Jul 2018

  The Joint Laboratory of Biotechnology and Enzyme Catalysis is an institution, which aims was to combine the research potentials of Institute of Catalysis and Surface Chemistry and Institute of Plant Physiology, Polish Academy of Sciences in the field of biochemistry and biotechnology. The main objective was to provide free access to research equipment for Laboratory researchers and to join the expertise in the field of chemistry, catalysis, biology and biochemistry.