Experience

Max Planck Institute of Biophysics

• Germany
• Research Services
• 1 - 100 Employee

• Postdoctoral Researcher

  • Jan 2022 - Present

  Visual Proteomics, Department of Molecular Sociology (Beck group)

• EM specialist

  • Jul 2021 - Present



The University of Edinburgh

• United Kingdom
• Research Services
• 700 & Above Employee

• CryoEM Technologist

  • Feb 2019 - Jun 2021

  I was responsible for running the cryoEM facility at the School of Biological Sciences. I made sure the cryoTEM is running optimally, trained and assisted users, and performed data collection. Projects mainly involved single-particle analysis and CLEM. I was responsible for running the cryoEM facility at the School of Biological Sciences. I made sure the cryoTEM is running optimally, trained and assisted users, and performed data collection. Projects mainly involved single-particle analysis and CLEM.



Leiden University Medical Center

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• PHD Student

  • Jan 2014 - Dec 2018

  During my PhD, I developed new techniques for Cryo Correlative Light- and Electron Microscopy. Specifically, I developed cryo super-resolution light microscopy, correlated with electron cryo-tomography. To develop cryo super-resolution, I build and adapted an optical set-up, actively participated in the development of acquisition software, carried out super-resolution experiments, set up an analysis pipeline and correlated super-resolution images with cryoEM images of the same region. Specifically, I addressed several challenges related to super-resolution of cryosamples, such as thermal drift of the stage, devitrification caused by high laser intensities and unknown properties of fluorescent probes at low temperature. Furthermore, I developed a new method for imaging high-pressure frozen & freeze substituted samples that utilities the increased fluorescence of uranyl-acetate (a commonly used EM-stain) at low temperatures. This can be used for intuitive sample navigation, overview of the cellular ultrastructure at the light microscopy level and to provide high-accuracy alingment between light and electron microscopy images. Show less



Delft University of Technology

• Netherlands
• Research Services
• 700 & Above Employee

• Research Assistant

  • Oct 2011 - Jul 2012

  As a part of the KNAW research assistant programme, I was involved in scientific research on solid-state nanopores, in the lab of prof. dr. Cees Dekker. The goal of the project was to recapture DNA-molecules in nanopores, and to that end I wrote a Labview-script, tested, and succesfully recaptured single DNA-molecules over a 1000 times. As a part of the KNAW research assistant programme, I was involved in scientific research on solid-state nanopores, in the lab of prof. dr. Cees Dekker. The goal of the project was to recapture DNA-molecules in nanopores, and to that end I wrote a Labview-script, tested, and succesfully recaptured single DNA-molecules over a 1000 times.



Delft University of Technology

• Netherlands
• Research Services
• 700 & Above Employee

• Student Assistant

  • 2009 - 2011

  Description: assisting first year students with: several practical courses; Classical Mechanics; Theory of Relativity; Inleiding Technische Natuurwetenschappen (PGO); and partly rewrote and revised the instruction manual for first years practical courses.

• Research Assistant

  • Aug 2009 - Oct 2009

  As continuation of bachelor thesis project, worked on the behavior of single-stranded DNA in solid-state nanopores, in the lab of Prof.Dr. C. Dekker.



C.S.G. De Lage Waard

• Papendrecht

• Teacher Physics; Chemistry and Technology

  • Aug 2006 - Jul 2009

  Function: secondary school teacher in: Physics; Chemistry and Technology in first, second and third grade of havo and vwo. Function: secondary school teacher in: Physics; Chemistry and Technology in first, second and third grade of havo and vwo.