Experience

Institute for In Vitro Sciences, Inc.

• United States
• Research Services
• 1 - 100 Employee

• Toxicologist / Study director

  • Nov 2021 - Present



University of Alabama at Birmingham

• Higher Education
• 700 & Above Employee

• Researcher V

  • Oct 2020 - Nov 2021

  ▪ Cell biology: culture of HCASMC, HUVECs, transfection siRNA, migration (Boyden chambers), apoptosis (Caspase-Glo® 3/7 assay and Tunnel assays), proliferation (Counting), studies in hypoxia, co-culure. ▪ Molecular biology: mRNA extraction, RNA quantification (nano-drop), and RT-qPCR. ▪ Animal skills: breeding, weaning, tail DNA genotyping. ▪ Lab management: participating in setting up new lab. ▪ Cell biology: culture of HCASMC, HUVECs, transfection siRNA, migration (Boyden chambers), apoptosis (Caspase-Glo® 3/7 assay and Tunnel assays), proliferation (Counting), studies in hypoxia, co-culure. ▪ Molecular biology: mRNA extraction, RNA quantification (nano-drop), and RT-qPCR. ▪ Animal skills: breeding, weaning, tail DNA genotyping. ▪ Lab management: participating in setting up new lab.



Johns Hopkins Medicine

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Postdoctoral Research Fellow

  • Feb 2019 - Oct 2020

  Study of pulmonary vascular physiology, molecular and cellular biology in Pulmonary Arterial Hypertension (PAH) ▪ Cell biology: Migration of cells using Transwell, apoptosis by altered nuclear morphology (Hoescht dye) and quantification by fluorescence microscopy, proliferation, culture of HLMVEC and HCMVEC, transfection SiRNA. ▪ Molecular biology: mRNA extraction, RNA quantification (nano-drop), and RT-qPCR. ▪ Biochemistry: Proteome profiler antibody array, Western Blot, Bradford and BCA. ▪ Lab management: Maintaining and organizing the cell culture room and the RT-qPCR machine. Training of other post-docs. Designated essential personnel for the lab during the COVID-19 quarantine. ▪ Analysis and reports: Presentation of results at weekly meetings. Experiment design from literature. Writing reviews on angiogenic factors in PAH. Study of molecular pathways implied in PAH. Show less



The National Institutes of Health

• United States
• Biotechnology Research
• 700 & Above Employee

• Postdoctoral Research Fellow

  • Feb 2016 - Feb 2019

  Oxidation of Methionine 77 in Calmodulin Alters Mouse Growth and Behavior ▪ Biochemistry: Protein expression and purification, protein-DNA binding assays, protoarray (Scanarray), Western blot, (co-)immunoprecipitation, ELISA. ▪ Molecular biology: -Vector design: primers design, digestions, ligations, cloning. -Cloning: transformation, reverse transcription, PCR, DNA purification (mini- and maxi-preps), DNA gel analysis, quantification and extraction, site-directed mutagenesis, sequencing. ▪ Microbiology: liquid and solid culture of bacterias ▪ Cell biology: cell culture (HEK-293, HeLa), DNA transfection ▪ Animal skills: breeding, tail DNA genotyping, study of the phenotyping behavior of transgenic mice. Titrations, spectrophotometry, chromatography (thin layer, HPLC), theoretical knowledge and interpretation of most analysis techniques (NMR, MS,…) Show less



Université de La Réunion

• France
• Higher Education
• 700 & Above Employee

• PhD student in Biochemistry and Cellular Biology

  • Feb 2011 - Nov 2014

  Study of the impact of antioxidant polyphenol-rich extracts from three medicinal plants on inflammatory and metabolic response of adipose cells in pathologies associated to obesity ▪ Cellular biology: cell culture (adipose cells 3T3-L1), differentiation, proliferation▪ Biochemistry: Western blot, ELISA, immunofluorescence, ▪ Molecular Biology: RNA extraction, RT- qPCR, PCR▪ Animal manipulation: Mice (C57Bl/6): Handling, feeding, breeding, organs dissection, OGTT▪ Analytical chemistry: plants extraction by organic solvent, phytochemical assays▪ Antioxidant activity assays: DPPH, ORAC, FRAP, DCFH-DA▪ Human nutrition: dietary polyphenols and phenolic acids in foods ▪ Oxidative stress, inflammation, metabolism, obesity, diabetes, adipose tissue, Show less

• Research and Teaching Assistant

  • Oct 2013 - Aug 2014

  ▪ Responsible for practical’s and tutorials for students in L1 Biology Chemistry



University of La Réunion

• Assistant researcher in fundamental research in organic synthesis

  • Jan 2010 - Jun 2010

  ▪ Analytical chemistry: chromatography on silica gel, CCM, SM, RMN, HPLC-MS, spectra analysis, ▪ Organic chemistry: Synthesis of bioactive molecular, ▪ Analytical chemistry: chromatography on silica gel, CCM, SM, RMN, HPLC-MS, spectra analysis, ▪ Organic chemistry: Synthesis of bioactive molecular,