Luciano Galdieri

Study Director at Eurofins Discovery
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Contact Information
us****@****om
(386) 825-5501
Location
St Louis, Missouri, United States, US
Languages
  • English Native or bilingual proficiency
  • Portuguese Native or bilingual proficiency

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Experience

    • United States
    • Biotechnology
    • 100 - 200 Employee
    • Study Director
      • Jan 2022 - Present

    • United States
    • Biotechnology Research
    • 100 - 200 Employee
    • Staff Scientist II
      • Mar 2021 - Dec 2021

      I independently design, carry out and perform procedures concurrently following all applicable regulatory (e.g. DEA, EPA, FDA, FIFRA, OECD) requirements defined by protocols, methods, and standard operating procedures (SOPs). I am responsible for the quality and accuracy of data under GLP and cGMP regulations and preparing reports to complete contracted work. I adhere to schedule according to the company’s or contracting organization’s timetable and establishes daily or weekly routines… Show more I independently design, carry out and perform procedures concurrently following all applicable regulatory (e.g. DEA, EPA, FDA, FIFRA, OECD) requirements defined by protocols, methods, and standard operating procedures (SOPs). I am responsible for the quality and accuracy of data under GLP and cGMP regulations and preparing reports to complete contracted work. I adhere to schedule according to the company’s or contracting organization’s timetable and establishes daily or weekly routines necessary to ensure on-time delivery. Additionally, I train staff and troubleshoot technical problems using equipment, developing formulas, processes, and methods. Show less I independently design, carry out and perform procedures concurrently following all applicable regulatory (e.g. DEA, EPA, FDA, FIFRA, OECD) requirements defined by protocols, methods, and standard operating procedures (SOPs). I am responsible for the quality and accuracy of data under GLP and cGMP regulations and preparing reports to complete contracted work. I adhere to schedule according to the company’s or contracting organization’s timetable and establishes daily or weekly routines… Show more I independently design, carry out and perform procedures concurrently following all applicable regulatory (e.g. DEA, EPA, FDA, FIFRA, OECD) requirements defined by protocols, methods, and standard operating procedures (SOPs). I am responsible for the quality and accuracy of data under GLP and cGMP regulations and preparing reports to complete contracted work. I adhere to schedule according to the company’s or contracting organization’s timetable and establishes daily or weekly routines necessary to ensure on-time delivery. Additionally, I train staff and troubleshoot technical problems using equipment, developing formulas, processes, and methods. Show less

    • Hospitals and Health Care
    • 700 & Above Employee
    • Postdoctoral Researcher
      • Oct 2016 - Mar 2021

      1. Mechanisms of Regulation of Cancer Stem Cell State. a. Dissociated patient specimens and murine specimens into single cells to study cancer stem cells (GSCs) subpopulations. b. Collected and analyzed mass cytometry (CyTOF) data to determine PI3K/AKT, MEK/ERK, WNT/b-catenin, JAK/STAT, MAPK/P38, and NFkB pathways activation status and downstream effectors. c. Used pathway inhibitor in vitro to determine the mechanism of increased clonogenicity and self-renewal of GSCs. d… Show more 1. Mechanisms of Regulation of Cancer Stem Cell State. a. Dissociated patient specimens and murine specimens into single cells to study cancer stem cells (GSCs) subpopulations. b. Collected and analyzed mass cytometry (CyTOF) data to determine PI3K/AKT, MEK/ERK, WNT/b-catenin, JAK/STAT, MAPK/P38, and NFkB pathways activation status and downstream effectors. c. Used pathway inhibitor in vitro to determine the mechanism of increased clonogenicity and self-renewal of GSCs. d. Collected ad analyzed mice survival. e. Performed statistical analysis and co-authored journal article (under review). 2. Functional Characterization of ZFHX4-CHD4 Interaction in Cancer Stem Cells a. Performed molecular cloning, transient protein expression and purification, co-immunoprecipitation, confocal microscopy, chromatin immunoprecipitation (ChIP), mRNA extraction, and qPCR b. Transduced and maintained stable cell lines expressing shRNAs or overexpressing proteins or peptide Show less 1. Mechanisms of Regulation of Cancer Stem Cell State. a. Dissociated patient specimens and murine specimens into single cells to study cancer stem cells (GSCs) subpopulations. b. Collected and analyzed mass cytometry (CyTOF) data to determine PI3K/AKT, MEK/ERK, WNT/b-catenin, JAK/STAT, MAPK/P38, and NFkB pathways activation status and downstream effectors. c. Used pathway inhibitor in vitro to determine the mechanism of increased clonogenicity and self-renewal of GSCs. d… Show more 1. Mechanisms of Regulation of Cancer Stem Cell State. a. Dissociated patient specimens and murine specimens into single cells to study cancer stem cells (GSCs) subpopulations. b. Collected and analyzed mass cytometry (CyTOF) data to determine PI3K/AKT, MEK/ERK, WNT/b-catenin, JAK/STAT, MAPK/P38, and NFkB pathways activation status and downstream effectors. c. Used pathway inhibitor in vitro to determine the mechanism of increased clonogenicity and self-renewal of GSCs. d. Collected ad analyzed mice survival. e. Performed statistical analysis and co-authored journal article (under review). 2. Functional Characterization of ZFHX4-CHD4 Interaction in Cancer Stem Cells a. Performed molecular cloning, transient protein expression and purification, co-immunoprecipitation, confocal microscopy, chromatin immunoprecipitation (ChIP), mRNA extraction, and qPCR b. Transduced and maintained stable cell lines expressing shRNAs or overexpressing proteins or peptide Show less

    • United States
    • Higher Education
    • 700 & Above Employee
    • Postdoctoral Associate
      • Feb 2011 - Sep 2016

      Intermediary Metabolism and Epigenetic Transcriptional Regulation a. Established acetyl-CoA as a link between fatty acid synthesis and epigenetic state b. Grew and maintained yeast and cancer cell lines c. Performed Western blot, chromatin immunoprecipitation (ChIP), mRNA isolation, qPCR, protein purification by affinity chromatography, enzyme kinetics, siRNA-mediated gene silencing, and ELISA. d. Used AMPK activators (Metformin and AICAR) to modulate epigenetic state. e… Show more Intermediary Metabolism and Epigenetic Transcriptional Regulation a. Established acetyl-CoA as a link between fatty acid synthesis and epigenetic state b. Grew and maintained yeast and cancer cell lines c. Performed Western blot, chromatin immunoprecipitation (ChIP), mRNA isolation, qPCR, protein purification by affinity chromatography, enzyme kinetics, siRNA-mediated gene silencing, and ELISA. d. Used AMPK activators (Metformin and AICAR) to modulate epigenetic state. e. Performed statistical analysis and co-authored 12 scientific articles Show less Intermediary Metabolism and Epigenetic Transcriptional Regulation a. Established acetyl-CoA as a link between fatty acid synthesis and epigenetic state b. Grew and maintained yeast and cancer cell lines c. Performed Western blot, chromatin immunoprecipitation (ChIP), mRNA isolation, qPCR, protein purification by affinity chromatography, enzyme kinetics, siRNA-mediated gene silencing, and ELISA. d. Used AMPK activators (Metformin and AICAR) to modulate epigenetic state. e… Show more Intermediary Metabolism and Epigenetic Transcriptional Regulation a. Established acetyl-CoA as a link between fatty acid synthesis and epigenetic state b. Grew and maintained yeast and cancer cell lines c. Performed Western blot, chromatin immunoprecipitation (ChIP), mRNA isolation, qPCR, protein purification by affinity chromatography, enzyme kinetics, siRNA-mediated gene silencing, and ELISA. d. Used AMPK activators (Metformin and AICAR) to modulate epigenetic state. e. Performed statistical analysis and co-authored 12 scientific articles Show less

Education

  • St. John's University
    Doctor of Philosophy (Ph.D.), Biochemistry and Molecular Biology
    2006 - 2011
  • Universidade Federal de São Paulo
    Master of Science (M.Sc.), Metabolism and Genetics
    2002 - 2004
  • Unifesp - Universidade Federal de São Paulo
    Bachelor of Science (B.Sc.), Biochemistry and Molecular Biology
    1996 - 1999

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