Lotte Carr
Head of Applications Development and Cytometry at Cellular Highways- Claim this Profile
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Bio
Experience
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Cellular Highways
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United Kingdom
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Biotechnology Research
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1 - 100 Employee
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Head of Applications Development and Cytometry
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Oct 2023 - Present
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Cytometrist
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Jan 2023 - Oct 2023
- Worked across a multidisciplinary team as the cytometry expert in the development of the Highway1 cell sorter.- Organised beta placements and visited external collaborators, both in academia and industry as part of the Highway1 beta development.- Exhibited at conferences, including Cyto2023 and SLAS2023, acting as a point of contact for potential clients and investors.
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The Francis Crick Institute
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United Kingdom
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Research Services
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700 & Above Employee
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Flow Cytometrist
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Mar 2019 - Jan 2023
Flow Cytometry Experience - Experienced in running and maintaining a range of flow cytomtetry analysers at CL1 and CL2, including BDLSRIIC, Fortessa, Symphony, Bio-Rad ZE5, Miltenyi MACS Quant, Beckman Coulter CytoFLEX, and Amnis ImageStream MkII. - Expertise sorting a range of biological material and clinical samples on MoFlo XDP, BD Influx, BD AriaIII and Fusions, and Propellabs Avalon at CL1, CL2, and CL3. - Specialised in MoFlo XDP machine modification to suit challenging sort samples. Carried out LASER alignment and replacement. Produced a poster and ran a workshop on enhancing MoFlo XDP resolution and quantifying LASER spot size at FlowCytometry UK meeting 2022. - Undertook basic training on Cytek Aurora, Sony ID7000 Standard Biotools Helios, Hyperion and XT mass cytometers. - Provided user training on all conventional analysers, as well as BD Aria Fusions, Propellabs Avalon, and FlowJo analysis. - Prepared and lead internal and external Flow seminars, as well as external practical training courses at the Francis Crick Institute. - Designed and ran a 10-day sort training course for a group leader and Ph.D. student from University of Cape Town. Was subsequently invited to Cape Town to give departmental training, talks and present at a conference, as part of the Crick African Network (CAN). COVID-19 Contributions - Helped to develop and carry out diagnostic assays for ELISA, Flow, and Mass Cytometry. - Generated SOPs and training for handling, processing and experimenting on clinical samples for COVID-19 research, for a number of studies including the nationwide VirusWatch study. Show less
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EMBL
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Germany
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Research Services
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700 & Above Employee
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Study in Industry Student
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Sep 2016 - Jul 2017
- Utilised a range of cell biology and cell labelling techniques including tissue culture, cDNA and siRNA transfection to study cellular trafficking. - Became proficient in widefield and high-throughput fluorescence microscopy. - Optimised a single-cell, protein trafficking assay and provided the Pepperkok team with improved protocols for RUSH assay. - Used ImageJ and CellProfiler to quantify protein trafficking of SEC23 coat proteins and presented data in a seminar to the Pepperkok team and Advanced Light Microscopy Facility. - Became the first undergraduate to present data at the Cell Biology and Biophysics departmental seminar. - Held a follow-up Q&A session and applied feedback to produce a first-class project report on the redundant role of SEC23A and SEC23B in E-Cadherin trafficking. Show less
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Education
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University of Bristol
Bachelor of Science - BSc, Biochemistry with Study in Industry -
The King's School Chester
A levels: Biology A*, Chemistry A*, Maths B, Physics A (AS) -
The King's School Chester
GCSEs: 8A*s, 2As