Experience

Systems Biology Ireland

• Ireland
• Biotechnology Research
• 1 - 100 Employee

• Laboratory Scientist

  • Dec 2022 - Present



ALMAC DIAGNOSTICS LIMITED

• United Kingdom
• Biotechnology Research
• 1 - 100 Employee

• Laboratory Scientist

  • Nov 2021 - Jul 2022

  Worked as a core laboratory scientist in the biomarker research group as part of Almac Diagnostics, experienced in Good laboratory practice protocols and laboratory record keeping and ensuring that any laboratory work I do follows GLP regulations. Experienced in H&E staining, RNA and DNA extraction processes and use of LIMS for laboratory sample processing. Trained in library preperation for total RNA sequencing using Roche Kapa kits. Worked as a core laboratory scientist in the biomarker research group as part of Almac Diagnostics, experienced in Good laboratory practice protocols and laboratory record keeping and ensuring that any laboratory work I do follows GLP regulations. Experienced in H&E staining, RNA and DNA extraction processes and use of LIMS for laboratory sample processing. Trained in library preperation for total RNA sequencing using Roche Kapa kits.



University College Dublin

• Ireland
• Higher Education
• 700 & Above Employee

• Graduate Research Student

  • May 2021 - Aug 2021

  The research project was conducted as part of my masters degree. The project involved investigating the impact of the SRF inhibitors CCG1423, CCG257081 and Lesta on the cell viability and proliferation of the androgen receptor negative DU145 prostate cancer cell line and the PWR1E prostate cancer cell line. SRF inhibitors have been proposed as potential treatments for castration resistant metastatic prostate cancers where androgen ablation therapies have failed. The research project was conducted as part of my masters degree. The project involved investigating the impact of the SRF inhibitors CCG1423, CCG257081 and Lesta on the cell viability and proliferation of the androgen receptor negative DU145 prostate cancer cell line and the PWR1E prostate cancer cell line. SRF inhibitors have been proposed as potential treatments for castration resistant metastatic prostate cancers where androgen ablation therapies have failed.



Cluain Mhuire mental health services

• Dublin, Ireland

• Research Assistant

  • Feb 2020 - Aug 2020

  Assist with research into mental health topics through researching assessment scale methods, writing literature reviews, aiding in the completion of ethics applications, compiling of gathered data and some statistical analysis of mental health diagnosis data from Irish databases. Assist with research into mental health topics through researching assessment scale methods, writing literature reviews, aiding in the completion of ethics applications, compiling of gathered data and some statistical analysis of mental health diagnosis data from Irish databases.



IMAC Group

• United Kingdom
• Facilities Services
• 1 - 100 Employee

• Surveyor

  • Nov 2019 - Nov 2019

  Worked as a surveyor for a UCD survey on room use for two weeks in November. The role involved checking a list of rooms in UCD each hour from 9 to 6 each day of the week to cheek if the room was occupied and give an estimate of how many people were in the room at the time. Each day a data sheet was handed back to the UCD estate services. The information gathered from the survey was to be used in the future development of buildings and site planning on campus. Worked as a surveyor for a UCD survey on room use for two weeks in November. The role involved checking a list of rooms in UCD each hour from 9 to 6 each day of the week to cheek if the room was occupied and give an estimate of how many people were in the room at the time. Each day a data sheet was handed back to the UCD estate services. The information gathered from the survey was to be used in the future development of buildings and site planning on campus.



Anyone 4 Science

• Ireland
• Primary and Secondary Education
• 1 - 100 Employee

• Camp leader

  • Aug 2017 - Aug 2019

  Duties included teaching groups ranging in age from senior infants to sixth class at weekly science camps during the summer months. Scientific topics had to be taught to these children in a way that was easy to understand and enjoyable for them, teaching was done using discussions and experiments. Duties included teaching groups ranging in age from senior infants to sixth class at weekly science camps during the summer months. Scientific topics had to be taught to these children in a way that was easy to understand and enjoyable for them, teaching was done using discussions and experiments.



Let's Go School Tours

• Dublin City University

• Climbing Instructor

  • Jun 2019 - Jun 2019

  The role involved giving safety talks to children's groups from the schools about wall safety, checking the equipment to ensure that it was safe for use and checking that the children's safety harnesses were put on correctly before allowing them to climb the wall. A key aspect of the role was to ensure that the groups had an enjoyable time and were kept safe during the activity. We would have each group for approximately 20 minutes during the day. The role involved giving safety talks to children's groups from the schools about wall safety, checking the equipment to ensure that it was safe for use and checking that the children's safety harnesses were put on correctly before allowing them to climb the wall. A key aspect of the role was to ensure that the groups had an enjoyable time and were kept safe during the activity. We would have each group for approximately 20 minutes during the day.



UCD Conway institute

• County Dublin, Ireland

• Undergraduate Research Assistant

  • Sep 2018 - Dec 2018

  Final year undergraduate research project taken during my final year with Dr Margaret Worrall. Thesis title: Expression and Purification of Serpin B3 Mutants and effects on Cathepsin K inhibition. Attempted to produce and purify wild type and mutant serpin B3 proteins in an attempt to determine if an amino acid change from a phenylalanine to a proline at position 352 would result in improved inhibition of the cathepsin K enzyme. Gained experience with SDS PAGE preparation and analysis, PCR, bacterial transformation using the QIA plasmid prep method of Rosetta 2 and DH5-alpha cells, DNA gel electrophoresis, aseptic techniques, protein purification using the immobilised metal ion affinity chromatography method and preparation of various laboratory reagent stocks, Experienced in writing safety statements for laboratory procedures. Experienced with troubleshooting laboratory procedures. During my final year project I had to troubleshoot my PCR procedure as it was not producing the desired mutant serpin B3 sequence, this process involved altering the PCR process such as changing the number of cycles and altering the PCR mixture. The troubleshooting process was successful allowing for completion of the project. Show less



Dr David O Connell

• UCD Conway institute

• Internship

  • Jun 2017 - Jul 2017

  Undertook an internship for one month in Dr David O'Connell's lab in the UCD Conway Institute where I attempted to purify a clathrin based protein tag in E.Coli cells. The internship exposed me to bacterial cell culture preparation and protein expression induction techniques and SDS PAGE techniques. Undertook an internship for one month in Dr David O'Connell's lab in the UCD Conway Institute where I attempted to purify a clathrin based protein tag in E.Coli cells. The internship exposed me to bacterial cell culture preparation and protein expression induction techniques and SDS PAGE techniques.