Postdoctoral Researcher

• Oct 2021 - Present

Researcher PHD Student

• Apr 2017 - Sep 2021

Project:Cytokine Release Syndrome: Dissecting immune responses and exploring novel anti-cytokine strategies in syngeneic mouse modelsChimeric antigen receptor (CAR)-modified T cells and bispecific T cell-engaging antibodies have demonstrated dramatic clinical responses. These highly active immunotherapies rely on strong T cell activation. Certain patients develop a cytokine release syndrome (CRS) which has been correlated with both toxicity and efficacy. In addition to elevations in effector cytokines, such as IFNg, cytokines associated with macrophage activation syndrome, such as IL-6, may also be markedly elevated. CRS in patients is characterized by high fever, hypotension, hypoxia, and/or multiorgan toxicity. Global immunosuppression with corticosteroids may control some of these toxicities, but their potential to block T cell activation and abrogate clinical benefit is a concern. A better understanding of the mechanisms underpinning the cytokine storm may help in early management of CRS.

Research Associate

• Jul 2016 - Mar 2017

Missions:Member of Protein Expression team, I am involved in providing high quality target proteins and antibody candidates for pre-clinical assays. A second part of my job is to generate stable cell lines (in HEK, Jurkat, BaF or CHO cells) for research projects.On my free time remaining, I continue the research project that I have began during my master's thesis : develop a transient expression platform in CHO cells. Tools:- Molecular biology: vector construction (E.Coli expression, purification)SnapGene, Fasteris- PEI transfection / electoporation- Protein production in mammalian cells- Purification (Bench purification, AKTA)- Characterization with SDS PAGE, SEC, CGE

Master's thesis - Research Intern

• Jul 2015 - Feb 2016

Project:Development and optimization of a transient expression platform in CHO-S cells for recombinant protein research productionMissions:- PEI transient transfection process optimization- Culture process optimization- Cells metabolism investigation- Autonomous design, management and analysis of experiments- Write synthetic report and oral presentation of the internshipTools:- Molecular biology: vector construction SnapGene, Fasteris- Design of experiment and Statistical analysis of data (predictive mathematical models and profiler building) JMP software- Culture monitoring: Vicell, Nova, Guava (FlowJo) - Protein production: Octet, Guava- Protein quality attributes comparaisonKey words : CHO cells - Episomal replication - Transient transfection - Recombinant protein production - DoESupervisors :Dr DESOBRY Carole, Senior Research OfficerMs LAURENDON Amélie, Associate Scientist

Bacelor's thesis - Research Intern

• Jun 2014 - Aug 2014

Project: Aging and passed stress effects on the working memory (commonly called the "everyday life memory")Missions:- Validate the reproductibility of behavioral test on a radial maze- Confirm aging deficit on working memory- Understand mild stress effects on working memory- Autonomous management of management and analysis of experiments- Oral presentation of a scientific article (Newspaper Club)- Write report and oral presentation of the internshipTools:- Mouse handling- Slice into brains:Vibratome- Fos immunohistochemistry- Statistical analyses of data (ANOVA):StatView- BibliographyKey words:Working memory – Aging – Stress – Fos immunochemistry – Radial mazeSupervisors:Dr MARIGHETTO Aline, Reseacher - Team LeaderDr SELLAMI Azza, Post Doctoral Researcher

Research Intern (part-time) - TIPE project

• Jan 2013 - May 2013

TIPE is a project management done each year of French preparatory school. The goal is to establish a relevant problematic with the imposed annual theme and perform experiment to answer it. A half- day per week is dedicated to the project reflexion. Experiments are performed in parallel of theoretical courses, at school or in laboratory.Project: The preservation of the cut flowers: search for a similarity of action between Pokon and sugarMissions:- Establish a probelmatic- Design and planning relevant experients to answer the problematic- Adapt planning of experiments based on results obtained- Compare effects of contact fungicides and SDS (Stimulating of Natural Defense of plants)- Oral presentationTools:- CFU (Fungus, bacteria)- CMI- Isolations and characterization of micro-organisms from stalks of plants- Statistical analyses of data- Bibliography

Medical clerk

• Aug 2012 - Aug 2012

- Reception and receipt- Management of switchboard, parcels - Reception and receipt- Management of switchboard, parcels