Lisanne Smulders
Plantkundig onderzoeker at PlantResearch BV- Claim this Profile
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Dutch Native or bilingual proficiency
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English Professional working proficiency
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German Limited working proficiency
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Spaans Professional working proficiency
Topline Score
Bio
Credentials
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MSc certificate Plant sciences
Wageningen University & ResearchNov, 2016- Sep, 2024 -
BSc certificate Biology and Medical Laboratry Research
Saxion -
Spanish course certificate (level B1.2)
Escuela delengua
Experience
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PlantResearch BV
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Netherlands
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Research Services
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1 - 100 Employee
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Plantkundig onderzoeker
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Feb 2023 - Present
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Phd-student (aio)
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Jun 2018 - Sep 2022
Worked as a PhD in the Marie Skłodowska-Curie poject on microbe induced resistance against agricultural pests (MiRA). Defended in September 2022. Worked as a PhD in the Marie Skłodowska-Curie poject on microbe induced resistance against agricultural pests (MiRA). Defended in September 2022.
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ABZ seeds
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Netherlands
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Farming
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1 - 100 Employee
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Junior researcher/ technician
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Jun 2017 - Sep 2017
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Enza Zaden
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Netherlands
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Food and Beverage Manufacturing
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700 & Above Employee
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MSc internship seed pathology
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May 2016 - Oct 2016
Worked at the department of seed pathology. Mainly performed molecular work (RNA extraction, RT-qPCR), sequence analysis, infection assays of tomato. Research of pospiviroids. Worked at the department of seed pathology. Mainly performed molecular work (RNA extraction, RT-qPCR), sequence analysis, infection assays of tomato. Research of pospiviroids.
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Wageningen University & Research
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Netherlands
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Research Services
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700 & Above Employee
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MSc Thesis Project phytopathologie
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Sep 2015 - Mar 2016
The subject of my MSc thesis was transport of sRNA between host plant and pathogen during which I mainly worked on gene cloning and transient expression assays in Nicotiana benthamiana. First, I have prepared knockout constructs of genes which were thought to be involved in virulence of Verticillium dahliae. Then, these were used to create V. dahliae knockouts by transformation of the spores in order to check whether these cultures would still be able to infect plants. Also, I studied targeting of the predicted virulence genes by a plant miRNA through transient transformation of N. benthamiana leaves. For that purpose, I have prepared constructs expressing the target gene tagged with a GFP and constructs expressing the miRNA itself. Then, when there is targeting a reduced GFP signal would be observed. Show less
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RIVM National Institute for Public Health and the Environment
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Netherlands
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Research Services
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700 & Above Employee
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BSc thesis RIVM
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Feb 2014 - Jul 2014
Development of a mumps specfic IgA assay for testing of saliva samples on the Luminex and the Microarray in order to study mucosal immunity. Mainly worked on immunological assays and the optimisation of these assays. Development of a mumps specfic IgA assay for testing of saliva samples on the Luminex and the Microarray in order to study mucosal immunity. Mainly worked on immunological assays and the optimisation of these assays.
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BSc Internship Plant Research International
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Feb 2013 - Jul 2013
During this internship I have worked with plant cell and tissue culture to study gene expression changes during somatic embryogenesis, using transgenic fluorescent reporter lines in which candidate genes were tagged with fluorescent proteins. Target genes were involved in different steps of somatic embryogenesis such as genes required for hormonal signalling, root and shoot growth or embryo development. By following the expression of these genes using microscopy during embryogenesis a combining different fluorescent reporter genes I learned more about the genes which are involved in embryogenesis and how they affect each other. I have become experienced with stable transformation of Arabidopsis thaliana. I enjoyed this internship and learned a lot about early plant development. Also, during that internship and during my master thesis I have worked with gene cloning and plant transformation (stable transformation in A. thaliana and transient transformation in tobacco plants). Show less
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Education
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2014 - 2016
Wageningen University
Master of Science - MS, Plant pathology and plant breeding -
2010 - 2014
Saxion Hogescholen
Bachelor of Science (BSc), Biology and Medical Laboratory Research
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