Experience

Nuvance Health

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Pathology Research Associate

  • Oct 2014 - Present

  Rudy L. Ruggles Biomedical Research Institute



Branford Biosciences, LLC.

• Co Owner and Assay Development Scientist

  • Jun 2013 - Dec 2013

  Branford, CT Custom development and performance qualification of cell-based assays and immunoassays on several platforms to include: Meso Scale Discovery® (electrochemiluminescent based assays), single and multi array Bio-Plex® bead based assays, fluorescent and colorimetric immunoassays. Diagnose assay performance issues and offer novel solutions to improve assay performance or streamline workflow.



Immunoassay Development Scientist

• Private Consultant: SUPERNOVA DIAGNOSTICS

  • Oct 2012 - May 2013



The Institutes for Pharmaceutical Discovery

• Scientist II

  • 1997 - Oct 2012

  Highly motivated and organized individual with the ability to work with minimal supervision on concurrent projects Extensive experience in the development and qualification of immunoassays on several platforms to include: Meso Scale Discovery® (electrochemiluminescent based assays), single and multi array Bio-Plex® bead based assays, fluorescent and colorimetric immunoassays. Excellent ability to troubleshoot and diagnose assay performance issues and offer novel solutions to remedy or… Show more Highly motivated and organized individual with the ability to work with minimal supervision on concurrent projects Extensive experience in the development and qualification of immunoassays on several platforms to include: Meso Scale Discovery® (electrochemiluminescent based assays), single and multi array Bio-Plex® bead based assays, fluorescent and colorimetric immunoassays. Excellent ability to troubleshoot and diagnose assay performance issues and offer novel solutions to remedy or streamline workflow. Identified and developed innovative applications for the BioPlex multiarray reader. Worked as a key member of the scientific team responsible for the development, optimization and performance qualification of six multiplexed panels (43 different biomarkers) for the quantitation of biomarkers in murine plasma. Evaluated over 130 different antibodies to identify matched pairs and biotinylated over 100 individual antibodies. Analyzed relevant collections of patient samples in commercially available ELISA’s to determine potentially relevant urinary biomarkers. Supervised a junior staff member in the development of in-house ELISA’s. Identified 25 urinary proteins of interest and initially developed single-plex bead-based assays for 11 of the 25 analytes of interest. Determined appropriate urine populations to analyze in the assays for use in identifying those of statistical significance. Analyzed data generated in all assays, and performed statistical analyses (ANOVA and ROC) to determine significance. As a result, developed a multiplexed panel of 5 statistically significant urinary proteins to demonstrate potential clinical utility. Served as the primary point-of-contact on all technical and scientific issues involving external client assay development projects. Provided technical expertise to ongoing, in-house research studies. Maintained meticulous documentation of all experimental work and authored all final project reports. Show less



Bayer

• Associate Research Scientist

  • Aug 1996 - Dec 1997

  Involved in the planning and monitoring of clinical trials. Critical analysis and interpretation of study findings. Instrumental in writing 510(k) submissions to the FDA for multiple tumor markers (CA-125, CA 15-3 and AFP). Evaluated clinical and non-clinical performance of tumor markers using the automated Immuno 1™ system. Performed preliminary assay development to support in-house research and development of tumor markers. Extensive writing of abstracts and manuscripts for national… Show more Involved in the planning and monitoring of clinical trials. Critical analysis and interpretation of study findings. Instrumental in writing 510(k) submissions to the FDA for multiple tumor markers (CA-125, CA 15-3 and AFP). Evaluated clinical and non-clinical performance of tumor markers using the automated Immuno 1™ system. Performed preliminary assay development to support in-house research and development of tumor markers. Extensive writing of abstracts and manuscripts for national meetings. Working knowledge of SOP and UOP procedures for ISO 9001 certification. Show less



New York Medical College

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • May 1990 - Aug 1996

  Responsible for the set-up of tissue culture facility and establishment and maintenance of HUVEC primary cell lines. Implement protocols pertaining to regulation of radioactive materials, hazardous chemicals and personnel safety. Organize and support laboratory and act as purchasing agent. Assist Director in manuscript and grant preparations. Maintain laboratory equipment and ensure proper maintenance. Supervise and instruct new students and staff in general lab techniques and… Show more Responsible for the set-up of tissue culture facility and establishment and maintenance of HUVEC primary cell lines. Implement protocols pertaining to regulation of radioactive materials, hazardous chemicals and personnel safety. Organize and support laboratory and act as purchasing agent. Assist Director in manuscript and grant preparations. Maintain laboratory equipment and ensure proper maintenance. Supervise and instruct new students and staff in general lab techniques and procedures. Show less