Experience

Synklino

• Denmark
• Biotechnology Research
• 1 - 100 Employee

• Senior Scientist

  • Nov 2022 - May 2023



Dragonfly Therapeutics, Inc.

• United States
• Biotechnology Research
• 1 - 100 Employee

• Principal Scientist at Dragonfly Therapeutics, Inc.

  • May 2022 - Nov 2022

  Antibody purification and characterization | NanoDSF | Developability | Lead selection | Project biologic lead | Opt-In preparation and reporting

• Senior Scientist

  • Apr 2021 - May 2022

  Antibody purification and characterization | NanoDSF | PreDevelopability | Lead candidate nomination | Work flow management | Establishment of Protein science lab



Symphogen

• Denmark
• Biotechnology Research
• 100 - 200 Employee

• Downstream Scientist

  • May 2016 - Jul 2019

  • Downstream responsible for cross functional collaboration regarding purification of reference antibodies, antigens and medium scale repertoires towards selection of lead candidates • Planning, technician coordination, execution and reporting of downstream process development activities • Document preparation and evaluation of development reports, MPI and MPR for Tech Transfer to GMP manufacturing facility • SE-µHPLC analysis of in-process downstream development samples Show more • Downstream responsible for cross functional collaboration regarding purification of reference antibodies, antigens and medium scale repertoires towards selection of lead candidates • Planning, technician coordination, execution and reporting of downstream process development activities • Document preparation and evaluation of development reports, MPI and MPR for Tech Transfer to GMP manufacturing facility • SE-µHPLC analysis of in-process downstream development samples Show less • Downstream responsible for cross functional collaboration regarding purification of reference antibodies, antigens and medium scale repertoires towards selection of lead candidates • Planning, technician coordination, execution and reporting of downstream process development activities • Document preparation and evaluation of development reports, MPI and MPR for Tech Transfer to GMP manufacturing facility • SE-µHPLC analysis of in-process downstream development samples Show more • Downstream responsible for cross functional collaboration regarding purification of reference antibodies, antigens and medium scale repertoires towards selection of lead candidates • Planning, technician coordination, execution and reporting of downstream process development activities • Document preparation and evaluation of development reports, MPI and MPR for Tech Transfer to GMP manufacturing facility • SE-µHPLC analysis of in-process downstream development samples Show less



Novo Nordisk A/S, Haemophilia Biology group + University of Salzburg, Hans Brandstetter group

• Novo Nordisk Park, Måløv + University of Salzburg,

• International PostDoc

  • Oct 2013 - Jan 2016

  • Kinetic and biophysical characterization of 15 factor IXa variants including CD, thermal and chemical stability analysis, fluorescent labeling and emission analysis • Cloning, E. Coli expression, refolding and purification of > 30 coagulation factor IX variants • Crystallization and diffraction dataset collection of 23 individual complexes • Structure determination including molecular replacement, data refinement and structural analysis for 15 factor IXa variant… Show more • Kinetic and biophysical characterization of 15 factor IXa variants including CD, thermal and chemical stability analysis, fluorescent labeling and emission analysis • Cloning, E. Coli expression, refolding and purification of > 30 coagulation factor IX variants • Crystallization and diffraction dataset collection of 23 individual complexes • Structure determination including molecular replacement, data refinement and structural analysis for 15 factor IXa variant complexes. Results published in Biochemical Journal and presented at the 2015 International Proteolysis Society meeting (IPS2015) . Show less • Kinetic and biophysical characterization of 15 factor IXa variants including CD, thermal and chemical stability analysis, fluorescent labeling and emission analysis • Cloning, E. Coli expression, refolding and purification of > 30 coagulation factor IX variants • Crystallization and diffraction dataset collection of 23 individual complexes • Structure determination including molecular replacement, data refinement and structural analysis for 15 factor IXa variant… Show more • Kinetic and biophysical characterization of 15 factor IXa variants including CD, thermal and chemical stability analysis, fluorescent labeling and emission analysis • Cloning, E. Coli expression, refolding and purification of > 30 coagulation factor IX variants • Crystallization and diffraction dataset collection of 23 individual complexes • Structure determination including molecular replacement, data refinement and structural analysis for 15 factor IXa variant complexes. Results published in Biochemical Journal and presented at the 2015 International Proteolysis Society meeting (IPS2015) . Show less



Københavns Universitet - University of Copenhagen

• Denmark
• Research
• 700 & Above Employee

• Post Doc

  • Jun 2012 - Oct 2013

  • Characterization of minimal motif for substrate recognition with kinetic and thermal stability analysis • Identification of inhibitors through screening of a natural product compound library with kinetic analysis, IC50 determinations and mode of action studies • Crystallization of histone demethylases with identified natural compound inhibitors • Optimization of expression and purification conditions for higher yield and purity

• Research assistant

  • Jan 2012 - May 2012



Faculty of Pharmaceutical Sciences, University of Copenhagen

• Denmark
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• PhD-student

  • Oct 2008 - Oct 2011

  • Biophysical characterization including CD, thermal stability and fluorescence • Kinetic characterization of histone demethylases including development of assay protocols for cellular inhibition studies and in-vitro screening of various compound libraries • Co-crystallization with identified inhibitors, diffraction data collection and data analysis • Establishment of expression and purification protocols for enzyme preparation for crystallization purposes including: Sequence analysis… Show more • Biophysical characterization including CD, thermal stability and fluorescence • Kinetic characterization of histone demethylases including development of assay protocols for cellular inhibition studies and in-vitro screening of various compound libraries • Co-crystallization with identified inhibitors, diffraction data collection and data analysis • Establishment of expression and purification protocols for enzyme preparation for crystallization purposes including: Sequence analysis for construct design, cloning of expression vectors and test expression in E. Coli, P. Pastoris and insect cells, and protein purification using affinity tag purification, ion exchange, hydrophobic interaction and size exclusion chromatography • Establishment of a semi HTP protein production pipeline for cloning and test expression, including protocol development for parallel cloning strategies, construct verification and small scale test expression in E. Coli and by baculo virus mediated insect cell expression. Thesis title: Kinetic and structural analysis of JmjC histone demethylases for identification of new inhibitors Show less • Biophysical characterization including CD, thermal stability and fluorescence • Kinetic characterization of histone demethylases including development of assay protocols for cellular inhibition studies and in-vitro screening of various compound libraries • Co-crystallization with identified inhibitors, diffraction data collection and data analysis • Establishment of expression and purification protocols for enzyme preparation for crystallization purposes including: Sequence analysis… Show more • Biophysical characterization including CD, thermal stability and fluorescence • Kinetic characterization of histone demethylases including development of assay protocols for cellular inhibition studies and in-vitro screening of various compound libraries • Co-crystallization with identified inhibitors, diffraction data collection and data analysis • Establishment of expression and purification protocols for enzyme preparation for crystallization purposes including: Sequence analysis for construct design, cloning of expression vectors and test expression in E. Coli, P. Pastoris and insect cells, and protein purification using affinity tag purification, ion exchange, hydrophobic interaction and size exclusion chromatography • Establishment of a semi HTP protein production pipeline for cloning and test expression, including protocol development for parallel cloning strategies, construct verification and small scale test expression in E. Coli and by baculo virus mediated insect cell expression. Thesis title: Kinetic and structural analysis of JmjC histone demethylases for identification of new inhibitors Show less



Københavns Universitet - University of Copenhagen

• Denmark
• Research
• 700 & Above Employee

• Research assistant

  • Mar 2008 - Sep 2008

  I was working as a part time research assistant in Birthe B. Kragekunds group, SBiN-Lab, Department of Biology and as part time secretary for Flemming M. Poulsen, SBiN-Lab, Department of Biology, organizing the PhD Summer School in Protein Science, which took place in Spetses June 6-14 2008

• Secretary at the Protein science summer school

  • Mar 2008 - Jul 2008



Københavns Universitet - University of Copenhagen

• Denmark
• Research
• 700 & Above Employee

• Instructor, Protein Science A - practical lab course

  • Oct 2007 - Oct 2007

  The course is a third year course for biochemistry, biology, chemistry and nanotechnology students

• Pilot at the “Scientific Village” (Den naturvidenskabelige festival)

  • Sep 2007 - Sep 2007

  The Scientific Village is an annual event wich takes place as a part of the Scientific Festival. Departments from different danish universities each join this three day event with different stands to show some of the reasearch that takes place at the different institutions. School children come and visits this festival to get their first hands on experience with reasearch, and the pilots help guide the children through different simple experiments and give a short introduction to their… Show more The Scientific Village is an annual event wich takes place as a part of the Scientific Festival. Departments from different danish universities each join this three day event with different stands to show some of the reasearch that takes place at the different institutions. School children come and visits this festival to get their first hands on experience with reasearch, and the pilots help guide the children through different simple experiments and give a short introduction to their own research interests. This year the Scientific Village was also open for the public as part of a globsl event called "Science After Dark: Researchers' Night" where 150 cities all over Europe participated. http://www.dennaturvidenskabeligelandsby.dk/ http://www.youtube.com/watch?v=gz32uy_IHgo



Københavns Universitet - University of Copenhagen

• Denmark
• Research
• 700 & Above Employee

• Instructor, Protein chemistry and enzymology II - practical lab course

  • May 2007 - May 2007

  The course is a first year course for Molecular biomedicine students. The course is a first year course for Molecular biomedicine students.



PARKEN Sport & Entertainment A/S

• Denmark
• Entertainment
• 100 - 200 Employee

• Stall manager

  • Feb 2004 - Nov 2006

  As stall manager I was the person in charge of a team of 20 employees who worked in the stalls during football matches and concerts serving drinks and snacks to the visitors As stall manager I was the person in charge of a team of 20 employees who worked in the stalls during football matches and concerts serving drinks and snacks to the visitors



Københavns Universitet - University of Copenhagen

• Denmark
• Research
• 700 & Above Employee

• Student

  • 2004 - 2005



Cafe Rendez Vous

• Kitchen assistant

  • May 1998 - May 2001