Experience

IN8bio

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Scientist

  • Feb 2023 - Present

• Staff Scientist II

  • Feb 2020 - Feb 2023



The University of Alabama at Birmingham

• Higher Education

• Scientist I

  • Jan 2019 - Feb 2020

  ▪ Continued optimization for long-term gene correction of sickle cell mutation in human patient derived hematopoietic stem cells for pre-FDA clinical trial application ▪ Continued engineering and optimization of modified Cas9 proteins for better fidelity and safety

• Postdoctoral Researcher

  • Dec 2012 - Dec 2018

  ▪ Designed and developed a novel modified CRISPR/Cas9 genome editing technology that greatly improve cell viability and reduce on-target and off-target INDELs, US/International patent WO2016205703A1 & WO2016205680A1 filed. ▪ Purified wild type Cas9 and over a 20 modified version of Cas9 recombinant proteins for RNP delivery in genome editing experiments. ▪ Successfully performed CRISPR/Cas9 enhanced gene correction of sickle cell disease(SCD) mutation in human sickle patient… Show more ▪ Designed and developed a novel modified CRISPR/Cas9 genome editing technology that greatly improve cell viability and reduce on-target and off-target INDELs, US/International patent WO2016205703A1 & WO2016205680A1 filed. ▪ Purified wild type Cas9 and over a 20 modified version of Cas9 recombinant proteins for RNP delivery in genome editing experiments. ▪ Successfully performed CRISPR/Cas9 enhanced gene correction of sickle cell disease(SCD) mutation in human sickle patient derived iPSCs and human/mouse sickle hematopoietic stem cells with high efficacy. Anayzed the short-term and long-term outcome of the genome editing using digital droplet PCR(ddPCR), sanger sequencing and next-generation sequencing technologies. ▪ Cured a humanized SCD mouse model by transplanting corrected hematopoietic stem cells using modified CRISPR/Cas9 system. 2nd and 3rd serial transplantation vaditated (First-in-the -world, unpublished data). ▪ Successfully purified a list of recombinant proteins and human/mouse cytokines for stem cell expansion and function study including bFGF, IL-6, c-Myc, TLR-9, etc.

• Graduate Research Assistant

  • Dec 2007 - Nov 2012

  Ph.D student research in structural and molecular basis of nucleic acid binding proteins and their specific interactions with RNA/DNA molecules