Experience

iVexSol

• United States
• Biotechnology
• 1 - 100 Employee

• Associate Scientist, Cellular Mechanisms

  • Jun 2022 - Present

  In this role I have developed and optimized a method to adapt cells from adherent, serum dependent to suspension, serum free in under a week. I have supported the development of single cell cloning techniques using both manual techniques and automation.Additionally, I generated growth curves of all maintenance cultures post thaw from cryopreservation, maintaining detailed tracking sheets. I have also performed kill curve assays on all cell lines for each antibiotic in use, utilizing cell… Show more In this role I have developed and optimized a method to adapt cells from adherent, serum dependent to suspension, serum free in under a week. I have supported the development of single cell cloning techniques using both manual techniques and automation.Additionally, I generated growth curves of all maintenance cultures post thaw from cryopreservation, maintaining detailed tracking sheets. I have also performed kill curve assays on all cell lines for each antibiotic in use, utilizing cell counting and cell imaging to monitor cell death

• R&D Project Support

  • Feb 2022 - Jun 2022

  During this internship, I analyzed the performance of two cell counting devices by maintaining 2-3 flasks of HEK293 cells per cell counter based on the viable cell density and viability that the respective cell counter produced. Throughout this experiment, I monitored the pH, glucose, lactate, and cell morphology of each flask before and after subculturing. One method used to analyze the results was a small-scale lentiviral vector production experiment to determine if the variability of the… Show more During this internship, I analyzed the performance of two cell counting devices by maintaining 2-3 flasks of HEK293 cells per cell counter based on the viable cell density and viability that the respective cell counter produced. Throughout this experiment, I monitored the pH, glucose, lactate, and cell morphology of each flask before and after subculturing. One method used to analyze the results was a small-scale lentiviral vector production experiment to determine if the variability of the cell counting devices impacted infectious titer



Worcester Polytechnic Institute

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Research Assistant

  • May 2021 - Aug 2021

  Mentors: Susan Roberts and Cassandra Brzycki In this role I heterologously expressed putative paclitaxel transporters from Taxus chinensis in ABC transporter deficient strain of S. cerevisiae (AD1-8), confirming transformation results using RT-qPCR. I also contributed towards the optimization of a previously used lithium acetate yeast transformation protocol using S.cerevisiae (AD1-8) by studying numerous published protocols and performed functional activity assays to study paclitaxel… Show more Mentors: Susan Roberts and Cassandra Brzycki In this role I heterologously expressed putative paclitaxel transporters from Taxus chinensis in ABC transporter deficient strain of S. cerevisiae (AD1-8), confirming transformation results using RT-qPCR. I also contributed towards the optimization of a previously used lithium acetate yeast transformation protocol using S.cerevisiae (AD1-8) by studying numerous published protocols and performed functional activity assays to study paclitaxel uptake and quantified intracellular and extracellular levels of paclitaxel using UPLC Show less Mentors: Susan Roberts and Cassandra Brzycki In this role I heterologously expressed putative paclitaxel transporters from Taxus chinensis in ABC transporter deficient strain of S. cerevisiae (AD1-8), confirming transformation results using RT-qPCR. I also contributed towards the optimization of a previously used lithium acetate yeast transformation protocol using S.cerevisiae (AD1-8) by studying numerous published protocols and performed functional activity assays to study paclitaxel… Show more Mentors: Susan Roberts and Cassandra Brzycki In this role I heterologously expressed putative paclitaxel transporters from Taxus chinensis in ABC transporter deficient strain of S. cerevisiae (AD1-8), confirming transformation results using RT-qPCR. I also contributed towards the optimization of a previously used lithium acetate yeast transformation protocol using S.cerevisiae (AD1-8) by studying numerous published protocols and performed functional activity assays to study paclitaxel uptake and quantified intracellular and extracellular levels of paclitaxel using UPLC Show less



EXPRESS

• United States
• Retail
• 700 & Above Employee

• Retail Associate

  • Jun 2018 - Dec 2019

  Interacted with customers in a sales context to promote products and assist customers as needed. Operated the register by checking customers out, signing customers up for credit cards, and assisting customers with online orders. Interacted with customers in a sales context to promote products and assist customers as needed. Operated the register by checking customers out, signing customers up for credit cards, and assisting customers with online orders.



Americas Compounding Center

• United States
• Cosmetics
• 1 - 100 Employee

• Pharmaceutical Intern

  • Apr 2018 - May 2018

  Observed laboratory experiments that focused on compounding medication (capsules, creams, gels, liquids), managed the front desk by receiving calls from clients with questions or requests to fill prescriptions, and aided in the transfer of paper to digital prescriptions. Observed laboratory experiments that focused on compounding medication (capsules, creams, gels, liquids), managed the front desk by receiving calls from clients with questions or requests to fill prescriptions, and aided in the transfer of paper to digital prescriptions.



Cones On the Common

• Grafton, MA

• Sales Associate

  • Jun 2017 - Oct 2017

  Greeted customers and aided in questions regarding merchandise, as well as food and beverage availability. Prepared and served food and beverages upon the customer’s request. Managed the cash register to assist with the customers with their purchase. Greeted customers and aided in questions regarding merchandise, as well as food and beverage availability. Prepared and served food and beverages upon the customer’s request. Managed the cash register to assist with the customers with their purchase.



Dunkin'

• United States
• Food and Beverage Services
• 700 & Above Employee

• Sales Associate

  • Jun 2016 - May 2017

  Greeted customers and aided in questions regarding food and beverage availability. Prepared and Served food and beverages upon the customer’s request. Managed the cash register to assist the customers with their purchase. Greeted customers and aided in questions regarding food and beverage availability. Prepared and Served food and beverages upon the customer’s request. Managed the cash register to assist the customers with their purchase.