Experience

HWC

• United States
• Business Consulting and Services
• 1 - 100 Employee

• Senior Associate

  • Apr 2015 - Present

  Washington D.C. Metro Area



The Henry M. Jackson Foundation for the Advancement of Military Medicine

• United States
• Research Services
• 700 & Above Employee

• Postdoctoral Fellow at NMRC

  • Jun 2013 - Apr 2015

  Washington D.C. Metro Area • Design and execution of assays to identify immunological factors, such as antibodies and antigen, in clinical samples that correlate to increased susceptibility to Staphylococcus aureus-associated skin and soft tissue infection. • Investigator of photodynamic therapy treatment as alternative to antibiotic treatment to against ESKAPE pathogens and invasive fungal infections. • Identification of bacteria in clinical wound samples using Phoenix diagnostic system. • Skilled in protocol… Show more • Design and execution of assays to identify immunological factors, such as antibodies and antigen, in clinical samples that correlate to increased susceptibility to Staphylococcus aureus-associated skin and soft tissue infection. • Investigator of photodynamic therapy treatment as alternative to antibiotic treatment to against ESKAPE pathogens and invasive fungal infections. • Identification of bacteria in clinical wound samples using Phoenix diagnostic system. • Skilled in protocol development and adaptation, clinical sample processing and testing, flow cytometry, Luminex BioPlex, tissue culture, and gram staining, clinical microbiology. • Supported strengthening of laboratory capabilities by assisting in standing up of new laboratory. Evaluation and procurement of laboratory equipment including biosafety cabinet, refrigerators, freezers, and pipettes. • Training of technicians of varying skill levels in biosafety, clinical sample processing and testing. • Certified in laboratory animal (rat and mouse) training. SKILLS -Multicolor flow cytometry (12 parameters). -Photodynamic therapy. -Experimental design and implementation. -Immunology. -Clinical microbiology. -Luminex. INSTRUMENTATION -BD LSRII -FACSDiva -FlowJo ADDITIONAL SKILLS -Collaboration. -Technical and grant writing. -Critical thinking and problem solving. -BSL-2 Show less



University of California, Davis

• United States
• Higher Education
• 700 & Above Employee

• Graduate Student Researcher

  • Sep 2008 - Jun 2013

  davis, california • Dissertation: The effects of HIV infection on antigen presenting cell phenotype and function. • Development of bacterial stimulation assays useful in evaluating the effects of HIV, chronic viral infection or inflammatory conditions on innate immune responses. • Design and implement multicolor flow cytometry panels and experiments. • Clinical coordinating following HIPAA requirements with study participants and clinical personnel. Facilitated recruitment and scheduling with study… Show more • Dissertation: The effects of HIV infection on antigen presenting cell phenotype and function. • Development of bacterial stimulation assays useful in evaluating the effects of HIV, chronic viral infection or inflammatory conditions on innate immune responses. • Design and implement multicolor flow cytometry panels and experiments. • Clinical coordinating following HIPAA requirements with study participants and clinical personnel. Facilitated recruitment and scheduling with study participants (HIV patients) and clinical staff. • Training of technicians and personnel of varying skill levels in biosafety, clinical sample processing and testing. • Skilled in virology, ELISA, Real-time PCR, immunohistochemistry, clinical sample testing, mucosal tissue collection and processing, worked in BSL-2 laboratory, with BSL-3 training. SKILLS • Development of ex vivo stimulation assays for functional responses of peripheral blood and mucosal immune cells (lung and GI) • Design and implement multicolor flow cytometry panels and experiments • Tissue collection (human and rhesus macaque) and processing • ELISA • Protein expression and purification • Cell culture • Mosquito rearing and dissection • PCR, RT-PCR • Immunohistochemistry • Western Blot Instrumentation • BD Biosciences LSRII Computer • SPSS • FlowJo • FACSDiva • GraphPad • Microsoft Office (Excel, Word, Powerpoint) Management/Leadership • Flow cytometry training • Biosafety and laboratory procedure training Show less



Tulane University

• United States
• Higher Education
• 700 & Above Employee

• Graduate Student Researcher

  • Sep 2006 - May 2008

  New Orleans, LA Graduate Student Researcher, Center for Infectious Diseases (October 2006-June 2007). • Advisor: Dr. Young Hong, Ph.D. • Investigated the effects of blt gene in Aedes aegypti ovary and egg development. • Extensive experience with RNAi construction and microinjection into Aedes aegypti, mosquito rearing, microdissection, insect cell (Sf9) culture, DNA and RNA isolation, PCR, virology (West Nile Virus).



University of Wisconsin-Madison

• Higher Education
• 700 & Above Employee

• Undergraduate Lab Technician

  • Oct 2003 - May 2006

  Madison, Wisconsin Undergraduate Research Assistant, Department of Oncology (January 2004-May 2006). • Advisor: Dr. Richard Burgess, Ph.D. • Epitope mapping of the beta subunit of RNA polymerase. • Extensive experience in protein expression and purification, Western blot, bacterial genetics, molecular cloning, aseptic technique.