Laura TZEPLAEFF

Postdoctoral Researcher at Technical University of Munich
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Contact Information
us****@****om
(386) 825-5501
Location
Munich, Bavaria, Germany, DE
Languages
  • Frensh Native or bilingual proficiency
  • German Native or bilingual proficiency
  • English Professional working proficiency
Skills

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Credentials

  • European certificat for animal experimentation and surgery
    University of Strasbourg
    May, 2018
    - Oct, 2024
  • Deutsch-Uni Online (DUO) German C1
    Ludwig-Maximilians-Universität (LMU) München
    Jun, 2014
    - Oct, 2024
  • Compétences en langues de l'enseignement supérieur (CLES) German B2
    Ministère en charge de l'enseignement supérieur et de la recherche (France)
    Jul, 2013
    - Oct, 2024

Experience

  • Technical University of Munich
    • Germany
    • Research Services
    • 700 & Above Employee
    • Postdoctoral Researcher
      • Sep 2022 - Present

      Postdoctoral position in the Lingor Lab from the Department of Neurology at the Rechts der Isar Hospital of the Technical University of Munich, Germany. Main project : In the scope of the international premodiALS consortium (www.premodials.med.tum.de) we will collect clinical data and biomaterials from presymptomatic gene mutation carriers at risk to develop ALS as well as early ALS patients and respective control subjects. Biomaterials will be analyzed with multi-omic approaches and results will be integrated with clinical and paraclinical data to generate a clinico-molecular fingerprint of presymptomatic and early ALS. Show less

  • Laboratory of Cognitive and Adaptative Neuroscience - University of Strasbourg
    • Strasbourg Area, France
    • Ph.D. Student
      • Oct 2017 - Dec 2021

      4-years and 3 months PhD thesis with Dr. Anne-Laurence Boutillier in the epigenetics and dynamics of memory systems research unit of the Laboratory of Cognitive and Adaptative Neuroscience (LNCA) - UMR 7364 - CNRS, University of Strasbourg, Strasbourg, France. Title of the PhD Thesis: Memory dysfunctions and associated epigenetic and transcriptomic changes in an Amyotrophic Lateral Sclerosis (ALS) & Frontotemporal Dementia (FTD) mouse model linked to a FUS mutation Jury members: Prof. Dr. André Fischer, Prof. Dr. Ludo Van den Bosch, Dr. Elsa Suberbielle, Dr. Juliette Godin & Dr Laurent Monassier Tasks: • Molecular biology: Chromatin Immuno Precipitation (ChIP), Protein/DNA/RNA extraction, Assay for Transposase-Accessible Chromatin (ATAC), nuclear extraction, Fluorescent Activated Nuclei Sorting (FANS), PCR and RT-qPCR, Western blot, Comet assay. • Microscopy: Immunohistochemistry (DAB, fluorescence, Golgy staining), optical fluorescent microscope, apotome • Animal caring and behavior: Mice handing and caring (breading, weaning, chip injection, genotyping, peritoneal injection), memory test (Morris Water Maze, Double H maze, Novel object recognition, Object location, Object in place, Temporal order memory, Social recognition), anxiety test (Plus maze, Open field, Light/Dark Box, Social defeat), locomotor activity test (Actography), mice fixation via transcardial perfusion, mice dislocation • Other: Brain cutting with the cryostat Side projects & publications in preparation: • A kinetic study of spatial learning transcriptomic in normal and pathological adult mouse hippocampus • Epigenomic/transcriptomic signatures in the pathological FTD/ALS brain in response to the positive effect of environmental enrichment • ChIP-seq against the FUS protein in muscle cells (C2C12). (In collaboration with Luc Dupuis, INSERM 1118) • Behavioral characterization of a conditional C9ORF72 knockout in neurons. (collaboration with Nicolas Charlet-Berguerand, IGBMC Strasbourg) Show less

  • Central and Peripheral Mechanisms of Neurodegeneration (MCPN) - University of Strasbourg
    • Strasbourg Area, France
    • Ph.D. Student
      • Oct 2017 - Dec 2021

      4-years and 3 months thesis codirected by Dr. Luc Dupuis in the Characterization of the neuronal pathways involved in ALS symtoms research unit of the UMR_S Inserm 1118 - Central and Peripheral Mechanisms of Neurodegeneration (MCPN), Faculty of Medicin of the University of Strasbourg, Strasbourg, France. Title of the PhD Thesis: Memory dysfunctions and associated epigenetic and transcriptomic changes in an Amyotrophic Lateral Sclerosis (ALS) & Frontotemporal Dementia (FTD) mouse model linked to a FUS mutation Show less

  • University of Strasbourg
    • France
    • Higher Education
    • 700 & Above Employee
    • Student Teaching
      • Sep 2018 - Aug 2021

      Discussion of scientific topics in German and English with 1st & 2nd year bachelor students : • Creation & establishment of the course for the Frenco-German Life sciences class • Supervision of student oral presentation, article reading and debates, alumni invitation… Discussion of scientific topics in German and English with 1st & 2nd year bachelor students : • Creation & establishment of the course for the Frenco-German Life sciences class • Supervision of student oral presentation, article reading and debates, alumni invitation…

  • Universität des Saarlandes
    • Germany
    • Higher Education
    • 700 & Above Employee
    • Master Internship
      • Nov 2016 - Sep 2017

      10-months Master thesis in Pr. Dr. Trese Leinders-Zufall laboratory of the Department of Sensory and Neuroendocrine Physiology in the Center for Integrative Physiology and Molecular Medicine, Saarlandes University, Homburg, Germany. Title of Master thesis : "TRPC channels in Crh-neurons and their possible implication in the murine stress pathway". Direct supervisor : Pr. Dr. Trese Leinders-Zufall First reviewer: Pr. Dr. Trese Leinders-Zufall (CIPMM), second reviewer: Dr. Pascal Darbon (INCI) Grade : 1,0 (very good)) Tasks: Mice handling, social defeat, whole mous fixation via transcardial perfusion, dissection, brain slices using cryotome, immunohistochemistry, microscopy analysis. Participations: - Trained by the Animal Protection Committee of the UdS : "Handling, fixing, marking, gender determination in mice", "Routes of application and methods of killing laboratory animals humanely", "Sampling and anesthesia". - Lab meeting once a week - Lectures : "Environmental Refinement" Show less

  • UCLA
    • United States
    • Higher Education
    • 700 & Above Employee
    • Research Intern
      • Jul 2016 - Oct 2016

      3-months internship in Gal Bitan laboratory of the Department of Neurology in the David Geffen School of Medicine, UCLA, Los Angeles, California, USA. Direct supervisor : Dr. Ravinder Malik Study of the modulation of abnormal self-association of Cu/Zn superoxide dismutase 1 (SOD1) by novel small molecules in the context of amyotrophic lateral sclerosis (ALS). Tasks : Expression and purification of the wild-type SOD1 protein and the E21K-SOD1 mutant, the native metal-bound form and the apo form. (Saccharomyces cerevisiae yeast cell culture on solid and in liquid media, cell lysis, Ammonium sulfate precipitation, hydrophobic interaction column chromatography (HIC), activity assay, SDS-PAGE, dialysis, anionic exchange chromatography, protein concentration, size exclusion chromatography, BCA assay and flash freeze). Participations : - Trained by the Environment, Health & Safety training (EHS): "Laboratory safety fundamental concepts". Statue : Finished - Passed on tuesday July 26, 2016. - Lab meeting once a week (topic: ALS, Parkinson, Alzheimer...) - Journal club once a month - 4th Distalz International Workshop: "Inhibition of Protein-Protein Interactions in Alzheimer's Disease and related Proteinopathies" March 27-28 2017, Lille, France. Show less

  • Ecole normale supérieure
    • Research Services
    • 700 & Above Employee
    • Research Intern
      • May 2016 - Jul 2016

      5-weeks internship in Antoine Triller's research unit of the Institute of Biology (IBENS) École normale supérieure (CNRS UMR8197 - Inserm U1024), Paris, France. Direct supervisor : Dr. Philippe Rostaing Imaging of cortical synapses in mice. Correlative Light and Electron Microscopy (CLEM) using the PALM/STORM (Photoactivated localization microscopy /Stochastic Optical Reconstruction Microscopy) and the TEM (Transmission Electron Microscopy). Tasks : Cutting slices with vibratome, microtome and ultra-microtome, labbeling proteins using immunohistochemistry, pre- and post-fixation, inclusion, microscopic observation. Observations : In-utero electroporation, electrophysiology, whole mous fixation via transcardial perfusion. Participations: - Lab meeting every two weeks Show less

  • Universität des Saarlandes
    • Germany
    • Higher Education
    • 700 & Above Employee
    • Bachelor Internship
      • Mar 2015 - Jul 2015

      5-months Bachelor thesis in Pr. Dr. Uli Müller's research unit of the department of Physiology/Zoology & Neurobiology at ZHMB, Saarbrücken, Germany Title of bachelor thesis : "Effect of the phytosterol stigmasterol on learning and chromatine modifications in honeybees (Apis mellifera).„ Direct supervisor : Pr. Dr. Uli Müller First reviewer: Pr. Dr. Uli Müller (ZHMB), second reviewer: Pr. Thomas J. Bach (IBMP-CNRS) Grade : 1,3 (very good) Tasks : Behaviour analysis on honeybees (classical appetitive 1- and 3-trials conditioning, testing of responsiveness, habituation, and sensitization). Molecular analysis of brain samples (ELISA). Participations: - Lab meeting once a week Show less

  • Universität des Saarlandes
    • Germany
    • Higher Education
    • 700 & Above Employee
    • Research Intern
      • Sep 2014 - Sep 2014

      5-weeks internship in Prof. Dr. Uli Müller's research unit of the department of Physiology/Zoology & Neurobiology at ZHMB, Saarbrücken, Germany Direct supervisor : Prof. Dr. Uli Müller Tasks : Behaviour analysis on honeybees (classical appetitive 1- and 3-trials conditioning, testing of responsiveness, habituation, and sensitization). Molecular analysis of brain samples (ELISA, SDS-PAGA, Wester Blot). Participations: - Lab meeting once a week 5-weeks internship in Prof. Dr. Uli Müller's research unit of the department of Physiology/Zoology & Neurobiology at ZHMB, Saarbrücken, Germany Direct supervisor : Prof. Dr. Uli Müller Tasks : Behaviour analysis on honeybees (classical appetitive 1- and 3-trials conditioning, testing of responsiveness, habituation, and sensitization). Molecular analysis of brain samples (ELISA, SDS-PAGA, Wester Blot). Participations: - Lab meeting once a week

  • Ecole normale supérieure
    • Research Services
    • 700 & Above Employee
    • Research Intern
      • Jul 2010 - Jul 2010

      1-week internship in Antoine Triller's research unit of the Institute of Biology (IBENS) École normale supérieure, Paris, France. Direct supervisor : Dr. Philippe Rostaing. Observation : Culturing of hippocampal cells, electrophysiology, calcium imaging, transmission electron microscopy, fluorescence microscopy, microtome, cryotome, inclusions, studies on GFP transgenic Caenorhabditis elegans. 1-week internship in Antoine Triller's research unit of the Institute of Biology (IBENS) École normale supérieure, Paris, France. Direct supervisor : Dr. Philippe Rostaing. Observation : Culturing of hippocampal cells, electrophysiology, calcium imaging, transmission electron microscopy, fluorescence microscopy, microtome, cryotome, inclusions, studies on GFP transgenic Caenorhabditis elegans.

Education

  • University of Strasbourg
    Doctor of Philosophy - PhD, Neuroscience
    2017 - 2021
  • Faculty of Life Sciences - University of Strasbourg
    Master's degree, Cellular and Integrative Neurosciences (frensh-german double degree)
    2015 - 2017
  • Natural Sciences and Technology Faculty and Medicine Faculty - Saarland University
    Master's degree, Neurobiology (frensh-german double degree), 1.7 (Good)
    2015 - 2017
  • Faculty of Life Sciences - University of Strasbourg
    Bchelor, Life Sciences (frensh-german double degree)
    2012 - 2015
  • Natural Sciences and Technology Faculty and Medicine Faculty - Saarland University
    Bachelor's Degree of Sciences, Human and Molecular Biology (frensh-german double degree)
    2012 - 2015
  • Lycée Edouard Shuré
    Baccalaureate, Sciences in the german european section
    2009 - 2012
  • Collège Edouard Shurée
    French-German Bilingual general certificate of secondary education
    2005 - 2009

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