Experience

Massachusetts Audubon Society

• United States
• Entertainment Providers
• 1 - 100 Employee

• Teacher/Naturalist

  • Mar 2014 - Present



Massachusetts Institute of Technology

• United States
• Higher Education
• 700 & Above Employee

• Technical Associate Laboratory Manager, and Hybridoma Specialist

  • Nov 1982 - Jul 2014

  • Hybridoma Specialist -Specialized in the production of monoclonal antibodies to low molecular weight carcinogens and their DNA adducts -Immunized mice, developed screening assays -Produced antibodies in vitro, characterized and purified antibodies, generated antibody resin for immuno-affinity chromatography -Taught mini-course at MIT, “How to make a monoclonal antibody,” lectures and labs • Technical Associate/Laboratory Manager – Cancer Research and Toxicology -Responsible for establishing a multi-user, Core Cell Culture/Hybridoma Facility and Analytical Lab, procurement of equipment, materials and supplies, equipment maintenance and troubleshooting. -Provided cell culture and safety training for students and post docs, and offered consultations in technical problem solving and experimental cell model design -Designed and performed toxicology mutation analyses in cell and animal models (mice) -Devised unique coculture system using Costar Transwells -Performed feasibility studies on nanoparticles (carbon nanotubes) to develop a novel nitric oxide in vivo sensor system resulting in initiation of a research project and publication -Facilitated communication between collaborators and projects, strong organizational skills and abilities to manage multiple project initiatives simultaneously and work independently -Prepared and submitted manuscripts for publication; formatted and created figures for data analysis and slides for presentation -Proficient in Microsoft Office and SAPweb • Selected Laboratory Skills and Techniques -Assay development -Isolation and culture of primary cells, production of mouse embryonic fibroblasts (MEFs), generation of stable transfected cell lines, culture of cells under hypoxic conditions -ELISA, RIA, Western blots, flow cytometry -Fluorescent and luminescent cell assays -DNA and protein isolation from cells and tissues -Exposure of cells to nitric oxide, analysis of nitric oxide metabolites by Nitric Oxide Analyzer Show less



Boston University School of Medicine

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • 1980 - 1982