Experience

UK Dementia Research Institute

• United Kingdom
• Research
• 100 - 200 Employee

• Research Assistant

  • Nov 2021 - Present

  Providing long read sequencing services for research and industry projects. Providing long read sequencing services for research and industry projects.



Great Ormond Street Hospital for Children NHS Foundation Trust

• United Kingdom
• Hospitals and Health Care
• 700 & Above Employee

• Clinical Scientist

  • Aug 2019 - Oct 2021



The Doctors Laboratory

• United Kingdom
• Medical Practices
• 500 - 600 Employee

• • Sep 2017 - Jan 2018

  Report preparation and authorisation for a UKAS accredited genetics laboratory.

• • Jul 2011 - Sep 2017

  Sample receipt, data entry, nucleic acid extraction from various sample types, assay set-up, analysis by various methods, training colleagues, examination audits, assay and equipment verification, SOP writing, competency assessing colleagues, participation in QA schemes, stock control, problem solving and department fire warden.Assays:o Apolipoprotein E genotyping – detection of ε2, ε3, and ε4 alleles.o Bacs-on-Beads KaryoLite and Prenatal testing.o HLA B27 screening o Coeliac screening - HLA alleles linked to an increased predisposition to the disease.o Cytochrome P450 - 2C19 and 2D6 genotyping.o Cystic Fibrosis 139 mutations NGS assay o Duchenne/Becker Muscular Dystrophy - deletions/duplications in DMD gene by MLPA.o Gastro-Intestinal Pathogen Panel - 15 gastro-intestinal pathogens in a multiplex.o Haemochromatosis genotyping - C282Y and H63D mutations in the HFE gene.o HLA tissue typing o Herpes Simplex Virus typing - for distinguishing between HSV Type I and HSV Type II.o Mental retardation panel - copy number changes of regions causing a number of syndromes.o Multiplex STI assay - 7 Sexually Transmitted Infections.o Narcolepsy screening - HLA alleles linked to increased risk of disease.o Paternity testing and human identification o Prader-Willi and Angelman syndrome testing - methylation specific PCR.o Prenatal QF PCR - detects common trisomies and sex chromosome aneuploidies.o Sanjad-Sakati/Kenny Caffey syndromes - 12 base pair deletion in TBCE gene. o Spinal muscular atrophy – copy number changes in Exon 7 of the SMN1 gene by MLPA.o Thrombophilia testing - common mutations in Factor II, Factor V, and MTHFR genes by ARMS PCR. o Uniparental disomy testing o Y chromosome screening - AZF microdeletions associated with male infertility. Show less

• • Oct 2010 - Jul 2011

  Sample receipt, data entry, trouble shooting, inter-departmental communication and team support.



King's College Hospital NHS Foundation Trust

• United Kingdom
• Hospitals and Health Care
• 700 & Above Employee

• Locum SATO-Specimen Reception

  • Apr 2010 - Oct 2010

  Sample receipt, data entry, dipstick urinalysis and sample preparation for biochemistry. Sample receipt, data entry, dipstick urinalysis and sample preparation for biochemistry.



Aotea Pathology Ltd.

• • Sep 2009 - Dec 2009

  DNA extraction, assay setup, analysis, stock-take, pipette calibration and environmental monitoring for the department.Assays:o Chlamydia screening - Real-time PCR.o Herpes Simplex Virus typing - for distinguishing between HSV Type I and HSV Type II by real-time PCR.o Haemochromatosis genotyping - C282Y and H63D mutations in the HFE gene by real-time PCR.o Human Papilloma Virus - detection of 37 HPV types by Roche linear array.

• • Jan 2007 - Dec 2009

  Sample receipt, data entry, call centre and troubleshooting.