Experience

Omniome

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist II, Manager

  • Mar 2019 - Present

  DNA clustering and sequencing biochemistry DNA clustering and sequencing biochemistry



Actavalon Inc.

• 3210 Merryfield Row San Diego Ca. 92121

• Research Scientist I

  • Oct 2016 - Mar 2019

  As one of Actavalon’s first FTE biologist I worked quickly to take a blank shell lab space to a fully functioning laboratory. I quickly integrated myself into our drug development pipeline by screening compound-protein interactions in vitro to provide critical SAR information for our medicinal chemistry team. As projects evolved into new areas of research, I frequently collaborated with a fellow biologist to design and implement a number of kit-based and de novo screens that covered a wide range of in vitro and cell based applications. • In vitro screening of compound libraries in high throughput format using recombinant proteins and fluorescence based assays • Screened lead compounds for targeted biological activity in cell based assays; including RT-qPCR, dual luciferase reporter gene assays, immunostaining, flow cytometry and fluorescence microscopy • Characterized the mechanism of action, target pathway and tissue specificity of an exciting novel compound series with unexplained specificity for a subset of cancers. • Designed and implemented multiple flow cytometry assays to evaluate compound activity in human T-cells • Prepared data and summary figures used to file multiple patent applications Show less



UC Irvine

• United States
• Higher Education
• 700 & Above Employee

• PhD Graduate Student

  • Apr 2011 - Oct 2016



California State University of San Marcos

• Research Technician

  • Aug 2009 - Jul 2011

  Responsible for managing the lab and performing research related to NIH and NSF-funded grants. Experience with DNA/RNA/protein isolation and analysis, enzyme activity assays, real-time PCR, GC-MS/HPLC analysis of small metabolites, reactive oxygen species assays, plant pathology assays, and genome-related bioinformatics. Assisted undergraduate students in understanding relevant lab concepts and skills Responsible for managing the lab and performing research related to NIH and NSF-funded grants. Experience with DNA/RNA/protein isolation and analysis, enzyme activity assays, real-time PCR, GC-MS/HPLC analysis of small metabolites, reactive oxygen species assays, plant pathology assays, and genome-related bioinformatics. Assisted undergraduate students in understanding relevant lab concepts and skills



SGX Pharmaceuticals

• Intern

  • Aug 2007 - Aug 2008

  Screening purified protein samples with multiple reagents for crystallization (coarse screen). Analysis and optimization of crystal growth conditions (fine screen). Skilled in TECAN, QIAgen (3000, 8000) and Art Robins Phoenix Robots; plus Microscopy, protein concentration and quantitation, and other relevant crystallization techniques. Screening purified protein samples with multiple reagents for crystallization (coarse screen). Analysis and optimization of crystal growth conditions (fine screen). Skilled in TECAN, QIAgen (3000, 8000) and Art Robins Phoenix Robots; plus Microscopy, protein concentration and quantitation, and other relevant crystallization techniques.



SGX Pharmaceuticals

• Biotechnology
• 1 - 100 Employee

• Intern

  • Jun 2007 - Aug 2007

  High-throughput cloning of bacterial genes in a BL21/T7 expression system for the Protein Structure Initiative (PSI). Purification/analysis of positive constructs for scale up. Process techniques: amplification PCR, agarose gel electrophoresis, analysis and extraction/purification of DNA, TOPO cloning and restriction based cloning into T7 6XHIS expression vectors, diagnostic screening for positive orientation clones, mini prep of DNA, transformation and expression in BL21 strains of E. coli, nickel purification of soluble proteins, analysis and glycerol stocks of positively selected clones based on size and level of solubility for scale up in fermentation. Show less