Kirsi Sepponen
Research And Development Specialist at SCellex- Claim this Profile
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Bio
Experience
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SCellex
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Finland
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Biotechnology Research
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1 - 100 Employee
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Research And Development Specialist
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Dec 2021 - Present
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University of Helsinki
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Finland
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Higher Education
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700 & Above Employee
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PHD Graduate Student
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Dec 2012 - Present
In vitro method development for differentiation of gonadal cell types and research of associated regulatory pathways. Methods: human pluripotent stem cell culturing and differentiation, RNA isolation and purification, reverse transcription, quantitative real-time PCR, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, molecular work for CRISPR Cas9 gene activation, cell transfection, single cell and whole sample RNA sequencing. In vitro method development for differentiation of gonadal cell types and research of associated regulatory pathways. Methods: human pluripotent stem cell culturing and differentiation, RNA isolation and purification, reverse transcription, quantitative real-time PCR, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, molecular work for CRISPR Cas9 gene activation, cell transfection, single cell and whole sample RNA sequencing.
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National Institute for Health and Welfare
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Helsinki, Finland
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Research Scientist
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Mar 2012 - Nov 2012
Microbiological research, development of an in vitro assay for microbiological research. Microbiological research, development of an in vitro assay for microbiological research.
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Finnish Red Cross Blood Service
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Helsinki, Finland
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Medical Laboratory Technician
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Nov 2011 - Feb 2012
Genome-based screening of human viruses from patient samples. Quality control of blood products by a flow cytometric assay. Genome-based screening of human viruses from patient samples. Quality control of blood products by a flow cytometric assay.
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Medical Laboratory Technician, Clinical Chemistry
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May 2011 - Aug 2011
Analysis of basic chemistry in patient samples by an immunoassay technique. Analysis of basic chemistry in patient samples by an immunoassay technique.
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Education
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University of Helsinki
Doctor of Philosophy - PhD Student, Developmental Biology, Reproduction, Stem Cell Research -
University of Jyväskylä
Master of Science - MS, Cell/Cellular and Molecular Biology -
University of Jyväskylä
Bachelor of Science - BS, Cell/Cellular and Molecular Biology