Experience

ImmunXperts, a Q² Solutions Company

• Biotechnology Research
• 1 - 100 Employee

• Scientist

  • Dec 2023 - Present

  Gosselies, Walloon Region, Belgium In direct contact with clients, scientist at ImmunXperts develop and perform high quality and innovative immunology-oriented experiments to cater to their needs and expectations.



ULB - Faculté des Sciences

• Postdoctoral Researcher

  • Oct 2023 - Dec 2023

  Gosselies, Walloon Region, Belgium I joined my PhD lab back for a couple of months to answer key reviewing questions about the research paper I submitted at the end of my thesis.



ULB - Faculté des Sciences

• PHD Student

  • Sep 2017 - Jul 2023

  Gosselies, Walloon Region, Belgium Subject : Regulation of innate and adaptive type 2 responses within adipose tissue in a mouse model of obesity: role of CD27 and PD-1 receptors I researched the role of innate lymphoid cells (ILC) and T cell function in adipose tissue homeostasis and obesity development (including type 2 diabetes) with a secondary focus on immune checkpoints (emphasis on PD-1 and CD27) and how they could be harnessed to prevent or treat obesity. I routinely used flow cytometry and cell sorting (FACS… Show more Subject : Regulation of innate and adaptive type 2 responses within adipose tissue in a mouse model of obesity: role of CD27 and PD-1 receptors I researched the role of innate lymphoid cells (ILC) and T cell function in adipose tissue homeostasis and obesity development (including type 2 diabetes) with a secondary focus on immune checkpoints (emphasis on PD-1 and CD27) and how they could be harnessed to prevent or treat obesity. I routinely used flow cytometry and cell sorting (FACS CANTO & ARIA III, Cytoflex LX), cell culture (ex vivo), PCR and qPCR. I also performed PCR cloning and directed mutagenesis, viral transduction, ELISA and sc-RNAseq. During my PhD I developed a wide array of skills : analytical thinking, problem solving, project management, autonomy, team work and supervision( (up to 3 long term student), scientific writing and communication, resilience… This project lead to the publication of a first paper in 2018 and a second one is currently under reviewing. Show less



Faculty of Medical and Health Sciences - University of Auckland

• New Zealand
• Higher Education
• 1 - 100 Employee

• Research Assistant

  • May 2017 - Jul 2017

  Auckland, New Zealand I joined the Fraser group which worked on the development of a new "pilvax" vaccine method against staphylococcus aureus. The concept was to clone highly immunogenic epitopes from S. aureus inside the pili strucutal proteins of the innocuous bacteria lactococcus lactis. The resulting plasmid would be use to generate a transgenic L. lactis which expressed a very high number of vaccine antigens as the bacteria would be covered by hundreds of pili, each of them consisting of multiple copies of the… Show more I joined the Fraser group which worked on the development of a new "pilvax" vaccine method against staphylococcus aureus. The concept was to clone highly immunogenic epitopes from S. aureus inside the pili strucutal proteins of the innocuous bacteria lactococcus lactis. The resulting plasmid would be use to generate a transgenic L. lactis which expressed a very high number of vaccine antigens as the bacteria would be covered by hundreds of pili, each of them consisting of multiple copies of the recombinant structural protein. Vaccination would occur through intranasal administration to elicit strong mucosal immunity. I was specifically involed in 1 : cloning 3 peptide candidates into the plasmid (restriction cloning) 2 : transform L. lactis with each resulting plasmid (electric shock transformation) 3 : assess peptide expression in the pili (western blot) Show less



ULB - Faculté des Sciences

• Master Thesis

  • Sep 2016 - May 2017

  Brussels Region, Belgium Title : "Study of tRNA substrate recognition by TrmK methyltransferase from Bacillus subtilis" The aim of this project was to understand the specificity of the methyltransferase enzyme TrmK by using synthetic tRNA. TrmK catalyses the transfer of a methyl group on an adenosine residue at position 22. The enzyme aspecificity was assessed using 8 iso-acceptor. In order to study the mechanistic processes behind TrmK specificty we generated mutated tRNAs by swapping specific residues and… Show more Title : "Study of tRNA substrate recognition by TrmK methyltransferase from Bacillus subtilis" The aim of this project was to understand the specificity of the methyltransferase enzyme TrmK by using synthetic tRNA. TrmK catalyses the transfer of a methyl group on an adenosine residue at position 22. The enzyme aspecificity was assessed using 8 iso-acceptor. In order to study the mechanistic processes behind TrmK specificty we generated mutated tRNAs by swapping specific residues and remove whol features. This project allowed me to develop my technical skills in molecular biology, in particular cloning, bacterial cell culture and two-dimensional chromatography. I also perfected my scientific writing and presenting skills. Working mostly by myself in the lab allowed me to develop autonomy and self-confidence which helped me grow both as a scientist and a person. Show less