Kerilyn Higashi

Research Associate at Link Immunotherapeutics
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Contact Information
us****@****om
(386) 825-5501
Location
Greater Seattle Area

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Experience

    • United States
    • Biotechnology
    • 1 - 100 Employee
    • Research Associate
      • Aug 2022 - Present

    • United States
    • Professional Training and Coaching
    • 1 - 100 Employee
    • Team Advisor
      • Jun 2022 - Jul 2022

      Team Advisor for Health and Medicine Program at University of California, Los Angeles - Led, facilitated, and mentored high school students interested in health and medicine through leadership and health/medicine-related activities - Helped students refine their leadership skills and to approach "challenge by choice" - Created and taught lectures/facilitated activities for pathology/epidemiology (ELISA), eye dissections, mass casualty incidents, and vaccine administration Team Advisor for Health and Medicine Program at University of California, Los Angeles - Led, facilitated, and mentored high school students interested in health and medicine through leadership and health/medicine-related activities - Helped students refine their leadership skills and to approach "challenge by choice" - Created and taught lectures/facilitated activities for pathology/epidemiology (ELISA), eye dissections, mass casualty incidents, and vaccine administration

    • United States
    • Higher Education
    • 700 & Above Employee
    • Undergraduate Researcher
      • Sep 2021 - Jun 2022

      I worked on an independent research project in the Kaiser lab investigating a bacterial defense system called BREX (“bacteriophage exclusion”). My primary role was to characterize the DNA binding requirements of BrxR, a putative transcriptional regulator that is linked to a subset of BREX defense systems. Later on, my research was focused on the BrxR homolog in Vibrio cholera Ban9. We did not detect specific binding, but we were able to partially restore binding by making a point mutation in… Show more I worked on an independent research project in the Kaiser lab investigating a bacterial defense system called BREX (“bacteriophage exclusion”). My primary role was to characterize the DNA binding requirements of BrxR, a putative transcriptional regulator that is linked to a subset of BREX defense systems. Later on, my research was focused on the BrxR homolog in Vibrio cholera Ban9. We did not detect specific binding, but we were able to partially restore binding by making a point mutation in the BrxR amino acid sequence. We hope to determine which amino acid positions may be significant in DNA recognition and how the BREX defense mechanism is regulated. Publication: Yvette Luyten, Deanna Hausman, Juliana Young, Lindsey A. Doyle, Natalia C. Ubilla-Rodriquez, Abigail R. Lambert, Kerilyn Higashi, Corina Arroyo, Kevin Forsberg, Richard M. Morgan, Barry L. Stoddard, Brett K. Kaiser. “Identification and characterization of the WYL BrxR protein and its gene as separable regulatory elements of a BREX phage restriction system.” Accepted for publication at Nucleic Acids Research. Presentations: “Characterization of BrxR, a putative transcriptional regulator in the BREX bacterial defense system” - Presented at Murdock Undergraduate Research Symposium, Vancouver, WA | November 2021 - Presented to potential Seattle University donors, Seattle, WA | April 2022 Lab Techniques: - PCR and primer design - Gibson cloning - Mutagenesis - Electrophoretic mobility shift assay (EMSA) - Ni-NTA protein purification - ApE and PyMOL analysis - Multiple Sequence Alignment (MSA)

    • Undergraduate Teaching Assistant
      • Sep 2021 - Mar 2022

      Course: General Biology I Molecular & Cellular (BIOL 1610 and 1611) - Assist students during lab sections to ensure learning and a deeper understanding of lab procedures, techniques, and concepts - Grade student assignments and lab practicals and provide detailed feedback - Collaborate with the professor to make improvements on the class environment by providing one-on-one support and resources for extra support

    • United States
    • Professional Training and Coaching
    • 1 - 100 Employee
    • Team Advisor
      • Jul 2021 - Aug 2021

      Team Advisor for Health and Medicine Program at American University - Led, facilitated, and mentored high school students interested in health and medicine through leadership and health/medicine-related activities - Helped students refine their leadership skills and to approach "challenge by choice" - Taught lectures and facilitated activities for pathology, microscopy, and anatomy dissections Team Advisor for Health and Medicine Program at American University - Led, facilitated, and mentored high school students interested in health and medicine through leadership and health/medicine-related activities - Helped students refine their leadership skills and to approach "challenge by choice" - Taught lectures and facilitated activities for pathology, microscopy, and anatomy dissections

    • United States
    • Higher Education
    • 700 & Above Employee
    • Student Lab Assistant at Kaplan Lab
      • Sep 2019 - May 2020

      - Made solutions including buffers, media, and gels - Prepared Petri dishes - Autoclaved and cleaned dishware - Input data into Access

    • Research Intern at UCDNA Sequencing Facility
      • Sep 2019 - May 2020

      Applying wet lab and PCR skills to sequence DNA from labs around UC Davis Using Excel and Access to record customer data

    • United States
    • Professional Training and Coaching
    • 1 - 100 Employee
    • Assistant Team Advisor
      • Jul 2019 - Aug 2019

      Assistant Team Advisor for Health and Medicine Program at the University of Washington Lead and facilitated high school students interested in health and medicine through leadership and health/medicine related activities Assistant Team Advisor for Health and Medicine Program at the University of Washington Lead and facilitated high school students interested in health and medicine through leadership and health/medicine related activities

Education

  • Seattle University
    Bachelor of Science - BS, Molecular & Cellular Biology
    2020 - 2022
  • University of California, Davis
    Microbiology
    2018 - 2020
  • Redmond High School
    2014 - 2018

Community

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