Associate Principal Scientist

• Jun 2023 - Present

Graduate Researcher

• Jan 2016 - May 2023

Pseudomonas aeruginosa evasion of neutrophil antibacterial functions in early Cystic Fibrosis lung infection • Extensive experience and knowledge in microbiology and mammalian cell cultivation • Develop high throughput functional assays to test the efficacy of therapeutic monoclonal antibody treatment against bacterial infection, eg. neutrophil bacterial infection, bacterial neutrophil adhesion assay, opsonophagocytosis killing assay (Collaboration with AstraZeneca) • Immunology: Perform various host cell and bacterial surface/ intracellular differential staining, acquire, and analyze samples by flow cytometer and flowJo; immunofluorescence: stain samples, acquire images using LSR confocal microscope and analyze them using image J or automated cell counting software Icy • In vivo animal models: familiar with various mouse models, including infection, handling, and processing of mouse organs, as well as other pig and wound infection models • Biochemistry: quantify bacterial or host cell proteins using immunoblotting, ELISA • Molecular biology: nucleotide extraction (DNA, RNA and Plasmid), cDNA synthesis, RT-qPCR, molecular cloning, Gibson cloning technology, primer design, PCR, gel electrophoresis, restriction enzyme digest, DNA ligation, promoter-reporter design and expression measurement • Cell purification and isolation: isolate and purify both primary human and mouse neutrophils • Virology: isolated and worked with bacterial phages, plaque assay • Supervisor: mentored and trained undergraduate and graduate students • Teamwork: assisted with colleagues’ research projects and collaborated with multiple international external partners • Scientific writing: published in peer-reviewed journals, thesis, conducted literature reviews, and reviewed research articles with supervisor • Scientific evaluation: data analysis and experimental troubleshooting Show less

Visiting Graduate Researcher

• May 2018 - May 2018

Research Assistant

• Dec 2010 - Dec 2015

• Assisted postdocs and colleagues with their projects on identifying sRNA targets in Pseudomonas aeruginosa • Performed routine molecular techniques such as PCR, cloning, transformation, mating, cDNA synthesis, qPCR • Organized and managed laboratory inventories • Prepared various media for bacterial cultivation • Assisted postdocs and colleagues with their projects on identifying sRNA targets in Pseudomonas aeruginosa • Performed routine molecular techniques such as PCR, cloning, transformation, mating, cDNA synthesis, qPCR • Organized and managed laboratory inventories • Prepared various media for bacterial cultivation

Visiting Graduate Researcher

• Oct 2015 - Nov 2015

Understand the mechanism of Pseudomonas aeruginosa uses for host invasion and evasion Understand the mechanism of Pseudomonas aeruginosa uses for host invasion and evasion

Undergraduate Research Fellow

• Sep 2013 - May 2015

• Investigated the role of the orphan CRISPR system in Enterococcus faecalis • Performed phage enrichment assay to isolate bacterial phages • Performed plaque assay to quantify viral load • Investigated the role of the orphan CRISPR system in Enterococcus faecalis • Performed phage enrichment assay to isolate bacterial phages • Performed plaque assay to quantify viral load