Experience

Sciperio Inc

• United States
• Research
• 1 - 100 Employee

• Lab Manager

  • Dec 2019 - Present

  Job Responsibilities: • Direct and oversee the daily operational activities of the Sciperio Bio Lab o Maintain equipment, coordinate with vendors, order and maintain inventory, keep SDS logs and SOPs up to date • Manage project deadlines, deliverables, and budgeting • Research, design, and execute experimental outlines • Analyze, review, and collate generated data o Investigate deviations from expected outcomes of standard differentiation protocols • Develop, refine, and implement procedures and SOPs o Coordinate with collaborators to transfer these SOPs and assist in their implementation to standardize procedures and reagents used across program sites • Expand and differentiate human CD34+ primary cells from cord blood o In both static culture and proprietary stirred-tank bioreactor • Develop and perform assays including primary human cell culture, flow cytometry, Leishman and Giemsa staining, immunofluorescence, filtering and storing manufactured red blood cells Show less



St. Jude Children's Research Hospital - ALSAC

• United States
• Non-profit Organizations
• 700 & Above Employee

• Research Technologist

  • Oct 2013 - Dec 2019

  Job Responsibilities: • Assist Principal Investigator with the collection and analysis of data for specified research projects • Develop FFPE-IHC antibodies to screen for possible applicability in the clinical lab • Design and work up primers to validate point mutations, duplications, and fusions • Work up double-staining Immunofluorescence on FFPE tissues (as well as cells) to look at the symbiotic relationships of various antigens • Fresh tissue DNA/RNA extractions • FFPE DNA/RNA Extractions using the Maxwell® Instrument from Promega • Order and maintain all freezer logs and consumable materials • Keep up-to-date inventory for all samples processed in our lab (frozen tissue, unstained sections, DNA, RNA, protein) Show less



Sanofi Pasteur - VaxDesign Campus

• Orlando, FL

• Research Associate

  • Sep 2009 - Oct 2013

  Job responsibilities: • Review literature for related research developments and techniques • Demonstrates and trains others in specialized procedures • Protocol design based on experience and pertinent literature • Responsible for planning and scheduling research procedures • Analyzes and interprets results of studies • Record results of studies, compile and analyze data, prepare presentations, present findings to fellow technical staff and scientists • Expertise in performing the following techniques including but not limited to cell isolations, T cell culture, CD4 ICCS, CD8 ICCS, 8 color Flow Cytometry and analysis, ELISpot, Plaque Assay, PRNT, Immunostaining, Virus quantification and purification, blood processing, and maintenance of cell lines • Purchasing/Materials Manager o Monitor general inventory levels to ensure that quantities on hand are sufficient to meet the demands of the laboratories Show less



nScrypt Inc.

• United States
• Machinery Manufacturing
• 1 - 100 Employee

• Laboratory Technician

  • Jan 2008 - Sep 2009

  • R&D of micro-dispensing/direct printing process and development • Conduct training lasting 2-4 days for customers of nScrypts 3Dn series of machines • Development of product manuals and design of training documents for nScrypts 3Dn series of machines • US Patent: Dispensing Patterns Including Lines and Dots at High Speeds • R&D of micro-dispensing/direct printing process and development • Conduct training lasting 2-4 days for customers of nScrypts 3Dn series of machines • Development of product manuals and design of training documents for nScrypts 3Dn series of machines • US Patent: Dispensing Patterns Including Lines and Dots at High Speeds