Katie Puddefoot, PhD
Scientist at Metrion Biosciences- Claim this Profile
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Bio
Experience
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Metrion Biosciences
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United Kingdom
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Research Services
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1 - 100 Employee
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Scientist
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Jan 2023 - Present
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University of Leicester
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United Kingdom
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Higher Education
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700 & Above Employee
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Postgraduate Researcher
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Sep 2018 - Jan 2023
My PhD project is researching the role of the ubiquitin proteasome system in neuronal function. I am currently investigating how inhibiting the proteasome affects the normal function of motor neurons in the zebrafish spinal cord, by using whole-cell patch clamp technique. I also plan to use a variety of behavioural techniques to study how inhibition of the proteasome affects locomotion in the embryonic zebrafish. I am funded by the Midlands Integrative Bioscience Training Partnership. As part of this training, I spent 3 months working with Dr Mark Wall at the University of Warwick and a further 3 months as an intern at Oxford University Innovation. Show less
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Oxford University Innovation
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United Kingdom
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Investment Management
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1 - 100 Employee
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Intern
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Apr 2019 - Jun 2019
As part of BBSRC’s Professional Internships for PhD Students scheme, I completed 3 months at OUI. I learnt valuable skills in technology transfer, helping to commercialise university IP. I also helped to organise the OIS Digital Health event and prepared marketing materials for both Bio and BioTrinity 2019. This placement gave me a real insight into commercialising life science technologies and a brief insight into IP law. As part of BBSRC’s Professional Internships for PhD Students scheme, I completed 3 months at OUI. I learnt valuable skills in technology transfer, helping to commercialise university IP. I also helped to organise the OIS Digital Health event and prepared marketing materials for both Bio and BioTrinity 2019. This placement gave me a real insight into commercialising life science technologies and a brief insight into IP law.
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University of Warwick
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United Kingdom
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Higher Education
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700 & Above Employee
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Project Student
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Jan 2019 - Mar 2019
As part of my PhD training year for the Midlands Integrative Bioscience Training Partnership, I spent 3 months working as a project student in the lab of Dr Mark Wall at the University of Warwick. My project was focused on using patch-clamp technique to study thalamocorticol neurons in rat brain slices. I learnt skills in whole-cell patch clamp technique including both voltage clamp and current clamp. I also learnt to analyse data using Clampfit and MatLab. As part of my PhD training year for the Midlands Integrative Bioscience Training Partnership, I spent 3 months working as a project student in the lab of Dr Mark Wall at the University of Warwick. My project was focused on using patch-clamp technique to study thalamocorticol neurons in rat brain slices. I learnt skills in whole-cell patch clamp technique including both voltage clamp and current clamp. I also learnt to analyse data using Clampfit and MatLab.
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ProImmune
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United Kingdom
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Research
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1 - 100 Employee
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Lab Assistant
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Jun 2017 - Sep 2018
As a Lab Assistant at ProImmune, I worked within the Cellular Analysis Services REVEAL and ProVE team. I learnt valuable skills in cell culture, protein assays and flow cytometry. I was also responsible for the analysis of laboratory services data and for writing customer reports. As a Lab Assistant at ProImmune, I worked within the Cellular Analysis Services REVEAL and ProVE team. I learnt valuable skills in cell culture, protein assays and flow cytometry. I was also responsible for the analysis of laboratory services data and for writing customer reports.
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University of Bath
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United Kingdom
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Higher Education
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700 & Above Employee
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Student Ambassador
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Sep 2016 - May 2017
The key aspect of this job was to give campus tours to prospective new students and to answer any questions they had regarding the University or the Department of Biology and Biochemistry. The key aspect of this job was to give campus tours to prospective new students and to answer any questions they had regarding the University or the Department of Biology and Biochemistry.
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Oxford Immunotec
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United Kingdom
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Biotechnology Research
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100 - 200 Employee
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ODL TB Technician
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Jun 2015 - Jun 2016
As part of my BSc Biochemistry with Professional Placement, I spent 13 months working at Oxford Diagnostic Laboratories as an ODL Technician testing blood samples using the TSpot TB assay. I learnt skills in isolating PBMCs from whole blood and using these in an ELISpot assay. As part of this placement, I also undertook two individual projects working to improve the assay for smaller blood volumes and to streamline the cell counting process. As part of my BSc Biochemistry with Professional Placement, I spent 13 months working at Oxford Diagnostic Laboratories as an ODL Technician testing blood samples using the TSpot TB assay. I learnt skills in isolating PBMCs from whole blood and using these in an ELISpot assay. As part of this placement, I also undertook two individual projects working to improve the assay for smaller blood volumes and to streamline the cell counting process.
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Clarks
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United Kingdom
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Retail
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700 & Above Employee
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Sales Assistant
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Jul 2014 - Sep 2014
As a Sales Assistant I gained many skills including customer service and organisation. As a Sales Assistant I gained many skills including customer service and organisation.
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Northwick Park Medical Research Institute
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Northwick Park Hospital
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Work Experience Placement
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Oct 2012 - Oct 2012
Shadowing a doctor at the facility. Working on Stem Cell research, in particular to do with the larynx and blood vessels. Gained skills in histology. Shadowing a doctor at the facility. Working on Stem Cell research, in particular to do with the larynx and blood vessels. Gained skills in histology.
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University of Greenwich
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United Kingdom
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Higher Education
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700 & Above Employee
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Work Experience Placement
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Jun 2012 - Jun 2012
Placement carried out in the chemistry department. Using basic laboratory techniques. Looking specifically at HPLC. Attended a department meeting which covered some of the PhD students' work. Placement carried out in the chemistry department. Using basic laboratory techniques. Looking specifically at HPLC. Attended a department meeting which covered some of the PhD students' work.
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Education
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University of Leicester
Doctor of Philosophy - PhD, Neuroscience -
University of Bath
Bachelor of Science (BSc), Biochemistry -
Chatham Grammar School for Girls