Experience

Imagion Biosystems, Inc

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Scientist

  • Nov 2021 - Present



Proteogenomics Research Institute for Systems Medicine (PRISM)

• Research Services
• 1 - 100 Employee

• Research Scientist

  • Jan 2017 - Nov 2021

  1. Developed new tumor model specifically for target protein expression in tumor based on human tumor tissue expression data. 2.Expertise in surgical model development for lung metastatic tumors derived after surgical removal of primary mammary tumor. 2. Executed radioimmunotherapy studies in syngeneic mouse tumor models, rat lung metastatic models. 3. In vivo biodistribution studies using radiolabeled antibodies specific for tumor and lung targeting by measuring radioactivity in different organs in rats and mouse (Gamma counter). 4. Working experience with antibody drug conjugates (Toxin payload) for tumor therapy in Her-2 resistant tumors (human mammary carcinoma tumor cell line- JIMT-1 xenograft mammary tumor model). 6. Drug administration by various routes such as tail vein, intramuscular, subcutaneous, and intraperitoneal in rats and mouse. 7. Blood collection cardiac puncture, retro-orbital, tail vein, tissue harvest, and processing of tissue samples for histology. 8. Tumor volume measurement, tissue processing for various downstream analysis. 9. Confocal imaging tissue slides for biomarker identification. 10.SPECT/CT whole animal imaging for in vivo biodistribution of radiolabeled antibodies and nanoparticles for tumor and lung specific targeting. 11. Ex vivo CT/SPECT imaging different organs and tumors tissue for antibody conjugates biodistribution studies. 12. Intravital fluorescent imaging tumor spheroids implanted in dorsal window chamber in nude mouse. 13. Working experience with Nude, SCID, NSG, R2G2 and GEMMs mice. 14. Expertise in Bleomycin-induced pulmonary fibrosis model. Show less



UTHSC

• Memphis

• Instructor

  • Nov 2014 - Jan 2017

  • Executed small molecule efficacy testing in preclinical animal studies. • Developed and conducted in vitro and in vivo experiments using small molecule drugs to develop drug targets for acute lung injury, pulmonary hypertension. • Developed precision cut-live lung slices for confocal imaging. • Evaluated signal transduction pathways of LPS-induced inflammatory signaling in primary lung microvascular endothelial cells. • Handle more than 3 projects simultaneously and in addition, I have generated data for NIH RO1 grants. • Intravital imaging studies of microvascular endothelium in an intact isolated blood-perfused lung. • Performed complicated surgical procedures such as caecal ligation and puncture model for sepsis and administration of agents through intratracheal, intra-peritoneal, transdermal, subcutaneous, oral routes of drug administration, retro-orbital, and cardiac puncture based blood collection in the animals. • Alveolar surfactant secretion in an intact ex vivo isolated lung preparation by intravital imaging. • Implanted mini-osmotic pumps in mouse for sustained drug delivery for chronic studies. • Reviewed research grant proposals for other members of the research group. • Responsible for teaching of animal, cell culture and experimental protocols to postdocs, graduate students and technicians. • Presented novel research findings in multidisciplinary discussion sessions. • Submitted research proposal in American Heart Association for Scientist Development grant. • Submitted full research proposal for Arthritis Foundation 2016 Delivering on Discovery Solicitation. • Managed the lab’s research personals, every day operations and responsible for animal protocol submission. Show less



UT St. Francis Family Practice Center

• United States
• Hospitals and Health Care
• 1 - 100 Employee

• Postdoctoral Research Fellow

  • Oct 2010 - Oct 2014

  • Intra-vital imaging of ex vivo blood-perfused lung using confocal and wide-angle fluorescent microscopy. • Worked with live cells using fluorescent imaging technique. • Learned to do lung section for immunohistochemistry studies. • In vitro gene knockdown in lung microvascular endothelial cells. • Isolation and culturing of primary rat and mouse lung microvascular endothelial and epithelial cells. • In vivo gene knockdown in lung microvascular endothelium by lentiviral particles through tail injection in mouse and rats. • Instillation of agents into single alveoli by micropuncture technique in isolated ex vivo lung preparation. • Delivery of fluorescent dyes and agents into single lung microvessel by micropuncture technique. • Real-time measurement of intracellular calcium imaging in intact lung microvessels using fluorescent microscopy. • Isolation and culturing of primary rat and mouse lung microvascular endothelial and epithelial cells. • Studied ciliary beating (visualized using LSM710 confocal microscope) in an isolated tracheal preparation. Show less