Experience

Department of Environmental Health Engineering, Sri Ramachandra University

• Hospitals and Health Care
• 1 - 100 Employee

• Senior Research Scientist (Biomarker)-NIH

  • Mar 2017 - Present

  • Designed and executed method development in human participants who exposed to household air pollution in rural settings. • Beside environmental toxins, optimized and validated ELISA kits for various clinical biomarkers (Inflammatory, lung insult, oxidative stress, endothelial damage, mitochondrial damage). • Developed dried blood spot sample volumetric analysis and conducted analysis to see temporal variation (kinetic assays) on clinical biomarkers through immunoassay. • Successfully developed a method to quantify the short telomere length through RT-PCR from cord blood, maternal blood and dried blood spot samples. • Developed a method and being first of its kind to quantify the toxicity of air pollution through cytogenetics assay. • Prepared SOP for the established methods (POC testing, biological sample collection, processing, transport, storage and analysis). • Upkeep on functionality of biorepository. • Trained junior staffs on sample analysis and ensure strict adherence to GLP, quality parameters. • Field visit to supervise the technicians and other co-ordinators during biological sample collection to ensure strict adherence to SOP and uphold of quality parameters in maintaining the sample integrity • Performed statistical analysis on data sets using Excel, Graphpad Prism and R (Basic). • Rendered technical support to Core-lead in preparing project reports, presentation and publications. • Collaborated with other department clinicians, team members within and outside of the laboratory. Show less



V ClinBio

• India
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• Research Coordinator

  • Mar 2016 - Nov 2016

  Involved in development of biomarkers and responsible in molecular diagnosis Highlights Enhanced experience in genetic diagnosis through MLPA, Sanger sequencing Experienced in Immunophenotyping- FACS Calibur by handling acute leukemia samples , HLAB-27 ankylosing spondylitis, minimal residual disease, CD4, CD8- HIV infection and Cell cycle analysis. Had hands-on experience in handling Next Generation Sequencing Conducted laboratory studies to evaluate the efficacy of metabolite being isolated from different sources. Conducted cell and molecular assays through gene expression and protein expressions to understand the molecular mechanism of biologically validated metabolites. Having investigated the kinetic inhibition of free radicals through ELISA assay. Development of molecular cytogenetics technique (FISH and Micronuclei) for characterizing cellular toxicity. Utilized the leisure time more efficiently that results in two publications as a first and corresponding author in peer-reviewed journals. Being a key person and developed a proposal on isolation of circulating tumour cells through FACS/MACS for the efficiency of metronomic chemotherapy. Supervision and rendered technical support to supervisor in preparing project grants, presentation and publications. Show less



Sri Ramachandra University

• Higher Education
• 400 - 500 Employee

• Project Coordinator

  • May 2014 - Dec 2015

  Central Research Facility, Sri Ramachandra University, Porur, Chennai. (June, 2014 – Till-to-date) Highlights: • Significantly enhanced classroom learning environment and acting as a facilitator to promote student leadership in initiating and creating positive learning opportunities. • Assumed overall responsibility of a devoted educator covering continual educational development and assessment of students; effectively collaborated with all levels of staff members and foster quality relationship with students. • Monitored progress during training, providing feedbacks & reviewing student’s performance. • Interacting with medical colleges in and around India about CITI-India online ethical program. Show less



University of British Columbia

• Vancouver, Canada Area

• Post Doctorate

  • Jan 2012 - Dec 2014

  Highlights: • Initiated, designed and executed CIHR grant, understanding the pathological consequences of endothelial cells and vascular endothelial growth factor in cardiac allograft vasculopathy. • Developed and optimized the immunohistochemistry method from paraffin embedded blocks to understand the pathological consequences of endothelial and vascular injury. • Team member in investigating vascular smooth muscle cells involvement through immunoassays (immunocytochemistry, western blotting, ELISA) in cardiac allograft vasculopathy project. • Analyzed the challenges in developing diagnostic marker through western blotting for enterovirus-Coxsackie virus the myocarditis study. • Undertaken collaborative studies, and developed an immunohistochemistry method for specific biomarker relevant to bypass graft failure that results in publication in high impact peer-reviewed journal. • Performed statistical analysis on data sets using Graphpad. • Mentored graduate students on application of biological assays to study the molecular mechanism of allograft vasculopathy. Show less



Rajah Muthiah Medical College, Annamalai University

• Chidambaram

• Guest Lecturer

  • Jun 2009 - Dec 2011

  Delivered guest lecturer in Cardiology Department and took classes on key topics include: Topics Delivered : 1. Genetic counselling- a key for the prevention of cardiovascular disease & congenital heart defects 2. Prenatal diagnosis and prevention of birth defects 3. Introduction to Human genetics 4. Overview of Metabolic Disorders 5. Congenital anomalies Delivered guest lecturer in Cardiology Department and took classes on key topics include: Topics Delivered : 1. Genetic counselling- a key for the prevention of cardiovascular disease & congenital heart defects 2. Prenatal diagnosis and prevention of birth defects 3. Introduction to Human genetics 4. Overview of Metabolic Disorders 5. Congenital anomalies



Rajah Muthiah Medical College and Hospital

• Project Assistant

  • Jan 2010 - Sep 2011

  • Specialized in ICH guidelines, ethical regulatory, reporting serious adverse events, medical writing and operational aspects of clinical trials funded by academic institutions and government bodies. • Worked as Project assistant in Clinical trial Projects on Indian Cardiovascular Research and Advocacy Group (ICRAG) Ovations Chronic Disease Initiative – Coordinated by St. John’s Medical College, Bangalore Population Health Research Institute, PHRI, McMaster University, Canada. • UMPIRE - Use of a Multidrug Pill in Reducing Cardiovascular Events. European Commission funded, randomized controlled trial, collaboration, Centre for Chronic Disease Control (CCDC), New Delhi. • Outstanding interaction with CRO's (Contract research organizations) through oral and mail communications, completed PHRP (Protecting Human research participants) course, NIH training. Show less



Annamalai University

• Junior Research Fellow

  • Aug 2006 - Jul 2009

  • Performed studies and conducted field experiments on Vibriosis infection in Shrimp with different treatments in line with objectives of the project. • Evaluated the Vibriosis infection in shrimps through In-direct ELISA technique. • Analyzed antigen-antibody complex through dot-blot assay. • Provided insight on these findings and ensured key findings are shared with appropriate internal audiences about the developed assay. • Accountable for undertaking assignments in research & managed analytical method development support for the study. • Identified issues and formed hypotheses, formulating and implemented recommendations/solutions. Show less



Kanchi Kamakoti Child trust Hospital

• Cytogenetician & Genetic Counselor

  • Jul 2005 - Jun 2006

  Involved in genetic diagnosis and counseling through karyotyping for babies with genetic syndromes. Technically sound in identifying and diagnosis of chromosomal, chromatid type of aberrations from peripheral blood lymphocytes. • Experienced in bone marrow culture setup. • Gained broad knowledge in chorionic villi sampling and amniotic fluid culture for early detection of genetic disorders. Involved in genetic diagnosis and counseling through karyotyping for babies with genetic syndromes. Technically sound in identifying and diagnosis of chromosomal, chromatid type of aberrations from peripheral blood lymphocytes. • Experienced in bone marrow culture setup. • Gained broad knowledge in chorionic villi sampling and amniotic fluid culture for early detection of genetic disorders.