Karen Triff

Scientist at Universal Cells
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Contact Information
us****@****om
(386) 825-5501
Location
Bellevue, Washington, United States, US

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Experience

    • United States
    • Biotechnology
    • 1 - 100 Employee
    • Scientist
      • Jun 2021 - Present
    • United Kingdom
    • Pharmaceutical Manufacturing
    • 700 & Above Employee
    • Lab Lead
      • Feb 2019 - May 2021

      Miaimi, FL

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Staff Scientist
      • Feb 2021 - Apr 2021

      Miami, Florida, United States

    • Sr Research Associate
      • Feb 2017 - Jan 2019

      University of Miami High Throughput Genome Sequencing expert Manage an interdisciplinary Epigenetics Workgroup monthly meeting Develop and re-design methodologies to examine genomic and epigenomic features within minimal amounts of tissue, such as patient biopsies Collaborate with outside institutions, such as Memorial Sloan Kettering Cancer Center and Icahn School of Medicine at Mount Sinai to explore epigenetic features in cancer patient samples

    • Postdoctoral Fellow
      • Mar 2016 - Feb 2017

      Bryan/College Station, Texas Area • Developed and tested the new bioinformatics workflow for processing and interpreting genomic sequencing data (hundreds of raw data files avg 15-1 Gb ea) • Increased efficiency of data analysis by 20X • Simplified formula while improving accuracy for determining signal to noise ratio of in vivo genome-wide data • Develop novel methodology for use of mammary gland tissue in proteomic, RNA and DNA metadata.

    • United States
    • Higher Education
    • 700 & Above Employee
    • Ph.D.
      • Mar 2010 - Dec 2015

      --Characterize the epigenetic and chemoprotective effects from short chain fatty acids derived from fiber and 3-n polyunsaturated lipids derived from fish oil --Genome wide analysis of the rat colon to reveal site-specific differences in histone modifications and proto-oncogene expression --Adapt Chromatin Immunoprecipitation (ChIP-Seq) and RNA sequencing technique to in vivo rat colonic crypts. --Practice classical and novel molecular biology laboratory methodology: Western Blot… Show more --Characterize the epigenetic and chemoprotective effects from short chain fatty acids derived from fiber and 3-n polyunsaturated lipids derived from fish oil --Genome wide analysis of the rat colon to reveal site-specific differences in histone modifications and proto-oncogene expression --Adapt Chromatin Immunoprecipitation (ChIP-Seq) and RNA sequencing technique to in vivo rat colonic crypts. --Practice classical and novel molecular biology laboratory methodology: Western Blot, RT-qPCR, tissue culture, immunoprecipitation, animal handling, gel electrophoresis, mass spectroscopy, transmission electron microscopy ----Transcriptome and ChIP-seq bioinformatic analysis of in-vivo mammalian model exposed to carcinogenesis and fed a chemoprotective diet --Corroborate with professionals in other departments and fields such as Electronic Engineering, Statistics and Bioinformatics. --Teaching Assistant for 3 years in Biology and Scientific Principles of Nutrition Software and Equipment Experience: Standalone Basic Local Alignment Search (BLAST), Ingenuity Systems Pathway Analysis, Galaxy, Unix/Linux, Python, SQL Certifications: Transmission Electron Microscopy, Animal Handling, Radiation Safety, Bloodborne Pathogens Safety, Teaching Assistant Certificate Show less

    • Research Assistant
      • May 2007 - Aug 2008

      Greater Pittsburgh Area --Project: "The effects of the cytidine deaminase inhibitors, tetrahydrouridine and 5--fluoro-2’-deoxycitidine”. Qualified adjuvant effect of those molecules on the half life of the pancreatic cancer drug gemcitabine, and whether they mitigate the high percentage of relapse associated with that treatment for pancreatic cancer. --Design methods and data analysis for individual project. Methods included transfection, drug treatment and toxicity assays of mammalian cancer… Show more --Project: "The effects of the cytidine deaminase inhibitors, tetrahydrouridine and 5--fluoro-2’-deoxycitidine”. Qualified adjuvant effect of those molecules on the half life of the pancreatic cancer drug gemcitabine, and whether they mitigate the high percentage of relapse associated with that treatment for pancreatic cancer. --Design methods and data analysis for individual project. Methods included transfection, drug treatment and toxicity assays of mammalian cancer cells. --Participate in weekly interdisciplinary writing group of collaborators including professors in departments of Molecular Biology, Biostatistics, Pharmacology, Hematology and Oncology. Show less

Education

  • Texas A&M University
    Doctor of Philosophy (Ph.D.), Molecular Biology
    2008 - 2015

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