Experience

Los Angeles Trade Technical College

• United States
• Higher Education
• 300 - 400 Employee

• Biotech Representative - LACC District - Office of Workforce & Economic Development

  • May 2023 - Present

  As the biotech representative for LATTC's employee training program, I connect with companies to provide state funded training and development for working professionals at all levels with an emphasis in the biotechnology space. As the biotech representative for LATTC's employee training program, I connect with companies to provide state funded training and development for working professionals at all levels with an emphasis in the biotechnology space.



Amgen

• United States
• Biotechnology Research
• 700 & Above Employee

• Associate Scientist: PD Cell Line Development

  • Mar 2020 - May 2023

  Cell line development lead for both first-in-human (FIH) and biosimilar programs. My chief responsibility as associate scientist in cell line development is to generate stable, high expressing cell lines of various molecule modalities for programs and tech dev. Perform transfection, selection, and recovery of target molecule expressing cell line. Execute fed batches, submit top expressers for cloning and lay down PMCBs with top clones. Proficient in operation of the Biomek liquid handler system, ViCell cell counters and other lab equipment complimentary to advance cell culture activities. Show less



City of Hope

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Associate 2: Clinical Theranostics - Upstream Process and Assay Development Scientist

  • Jun 2017 - Mar 2020

  My responsibilities as a clinical upstream process development scientist includes generating transient and stable clones through electroporation of vectors into mammalian cell lines for the expression of cancer antigen targeting antibodies using hollow fiber or wave bag bioreactor systems. Establish clone and sub-clones through limited dilutions and clone screening via cell culture bio-profiler (Stat Profile Prime), ELISA, SDS-PAGE, IEF gels and HPLC. Establish working cell bank and manufacture antibodies in an onsite pre-clinical GMP facility. Conjugation of pre-clinical theranostic imaging agents with target antibodies via amine-reactive crosslinker chemistry (NHS-DOTA and NHS-dyes) for use in fluorescence assisted surgery. Show less



Baxalta

• United States
• Biotechnology
• 200 - 300 Employee

• Inoculum Lead/Senior Cell Culturist/Purification Operations(Baxter)

  • Oct 1999 - Mar 2016

  I have held the positions of inoculum lead, buffer prep lead and senior bioreactor operator in a FDA regulated biotech operation. Responsibilities include scaling spinner cultures from cell bank vial thaw, inoculation, production and harvesting of CHO cell based recombinant anti-hemophilic Factor VIII protein from 2500L bioreactors. Formulation of media and buffer solutions for support of Factor VIII production. Large scale protein purification using ion exchange and affinity column chromatography. Duties are performed in Class 100,000 and Class 10,000 clean rooms. Acting EHS representative for area operations. Responsibilities include full monthly internal audits and all issues involving EHS. Author QA reviewed and issued SOPs. Show less