Experience

RemeGen Biosciences

• United States
• Biotechnology
• 1 - 100 Employee

• Senior Scientist

  • Dec 2022 - Present



MacroGenics, Inc.

• United States
• Biotechnology Research
• 200 - 300 Employee

• Associate Scientist

  • Apr 2016 - Present

• Associate Scientist II

  • Aug 2021 - Aug 2022

  Developed and implemented functional assays addressing chemokine-driven immune cell recruitment. Proposed and established an accelerated immunization protocol reducing time to LN/myeloma fusion by 80%. Generated and screened ~120 campaigns (over 100,000 hybridomas) to advance pipeline and platforms. Routinely utilized methodologies including FACS, ELISA, IEF, SDS-PAGE, small scale antibody purification, maxiprep, transfection.

• Consultant

  • Apr 2015 - Apr 2016



Ablexis

• San Francisco

• Senior Research Associate

  • May 2013 - Jan 2015

  Performed high-throughput hybridoma fusions resulting in 4000+ hybridomas per screen. Characterized epitope diversity and validated antibody specificity by western blot and ELISA-based assays. Cloned and expanded over 150 target-specific hybridomas ahead of company deadlines. Performed high-throughput hybridoma fusions resulting in 4000+ hybridomas per screen. Characterized epitope diversity and validated antibody specificity by western blot and ELISA-based assays. Cloned and expanded over 150 target-specific hybridomas ahead of company deadlines.



Genentech

• United States
• Biotechnology Research
• 700 & Above Employee

• Senior Research Associate

  • Dec 2011 - Feb 2013

  Assessed the capacity of MEHD7945A (DAF) to block autocrine and paracrine HER3 signaling in vitro. Developed the protocol for performing IP-westerns for phospho-HER3 in fresh-frozen human tumors to support the DAF biomarker publication. Generated stable cell lines from immortalized bronchial epithelial cells using viral transduction for analyzing the role of LDHB in lung tumorigenesis.

• Senior Research Associate

  • Aug 2011 - Dec 2011

  Analyzed the effects of kinase inhibitors on cellular signaling pathways.



The University of Texas Southwestern Medical School

• Medical Practices
• 200 - 300 Employee

• Senior Research Associate

  • Feb 2005 - Jun 2011

  Developed and executed functional assays with tumor cells and primary endothelial cells including cytotoxicity, migration, proliferation, and receptor binding/activation assays. Developed ELISA-based assays to test antibody specificity and activity. Maintained transgenic mouse colonies. Helped design and execute tumor therapy studies in mice. Assisted principal investigator in generating and updating animal protocols. Trained incoming graduate and medical students as well as… Show more Developed and executed functional assays with tumor cells and primary endothelial cells including cytotoxicity, migration, proliferation, and receptor binding/activation assays. Developed ELISA-based assays to test antibody specificity and activity. Maintained transgenic mouse colonies. Helped design and execute tumor therapy studies in mice. Assisted principal investigator in generating and updating animal protocols. Trained incoming graduate and medical students as well as post-doctoral researchers and surgery fellows on lab techniques and animal procedures. Performed lab manager duties such as ordering laboratory supplies, organizing laboratory space, and ensuring EH&S laboratory compliance. Show less Developed and executed functional assays with tumor cells and primary endothelial cells including cytotoxicity, migration, proliferation, and receptor binding/activation assays. Developed ELISA-based assays to test antibody specificity and activity. Maintained transgenic mouse colonies. Helped design and execute tumor therapy studies in mice. Assisted principal investigator in generating and updating animal protocols. Trained incoming graduate and medical students as well as… Show more Developed and executed functional assays with tumor cells and primary endothelial cells including cytotoxicity, migration, proliferation, and receptor binding/activation assays. Developed ELISA-based assays to test antibody specificity and activity. Maintained transgenic mouse colonies. Helped design and execute tumor therapy studies in mice. Assisted principal investigator in generating and updating animal protocols. Trained incoming graduate and medical students as well as post-doctoral researchers and surgery fellows on lab techniques and animal procedures. Performed lab manager duties such as ordering laboratory supplies, organizing laboratory space, and ensuring EH&S laboratory compliance. Show less



BioLegend

• United States
• Biotechnology Research
• 400 - 500 Employee

• Associate Scientist

  • Jan 2003 - Jan 2005

  Maintained, screened, and subcloned hybridoma cell lines. Scaled up antibody production and purification for biomedical research. Tested purified antibodies using flow cytometry. Assisted in tapping mice for ascites. Maintained, screened, and subcloned hybridoma cell lines. Scaled up antibody production and purification for biomedical research. Tested purified antibodies using flow cytometry. Assisted in tapping mice for ascites.



Scripps Research

• United States
• Research Services
• 700 & Above Employee

• Associate Scientist

  • Jan 2002 - Jan 2003

  Performed immune cell purification from murine spleen and lymph nodes using nylon wool columns. Performed flow cytometry. Animal husbandry. Performed immune cell purification from murine spleen and lymph nodes using nylon wool columns. Performed flow cytometry. Animal husbandry.



Corvas International, Inc

• Associate Scientist

  • Jan 2001 - Jan 2002

  Routinely cultured mammalian cell lines. Performed small animal injections and basic surgery. Implemented fusion technology to develop a panel of antibodies specific to targeted proteins. Successfully scaled up antibody production and purification. Provided antibodies for large multi-dose in vivo studies. Routinely cultured mammalian cell lines. Performed small animal injections and basic surgery. Implemented fusion technology to develop a panel of antibodies specific to targeted proteins. Successfully scaled up antibody production and purification. Provided antibodies for large multi-dose in vivo studies.



The Hope Heart Institute

• United States
• Research
• 1 - 100 Employee

• Research Technologist II

  • Jan 1999 - Jan 2001

  Analyzed cell proliferation, extracellular matrix production, growth factor expression, and matrix metallo-protease expression through primary cell cultures. Produced recombinant protein in baculovirus and performed routine protein purification. Characterized a panel of monoclonal antibodies generated against a specific target. Propagated mammalian and insect cell lines. Maintained and characterized transgenic mouse colonies. Managed laboratory consisting of twenty members. Analyzed cell proliferation, extracellular matrix production, growth factor expression, and matrix metallo-protease expression through primary cell cultures. Produced recombinant protein in baculovirus and performed routine protein purification. Characterized a panel of monoclonal antibodies generated against a specific target. Propagated mammalian and insect cell lines. Maintained and characterized transgenic mouse colonies. Managed laboratory consisting of twenty members.



University of Washington

• United States
• Higher Education
• 700 & Above Employee

• Research Technologist I

  • Jan 1995 - Jan 1999

  Characterized transgenic mouse colonies. Maintained insect cell lines for recombinant protein purification and production. Purchased reagents and prepared stock solutions for laboratory. Characterized transgenic mouse colonies. Maintained insect cell lines for recombinant protein purification and production. Purchased reagents and prepared stock solutions for laboratory.