Experience

Technical University of Munich

• Germany
• Research Services
• 700 & Above Employee

• PhD in structural biochemistry @ Groll lab

  • Jan 2019 - Present

  Topic: Epigenetic modifications in the anticodon region of tRNAs fine-tune its stability and functionality. The modification N6-threonylcarbamoyladenosine (t6A) at position 37, adjacent to the anticodon stem-loop in tRNA, is well understood, however the cyclization to ct6A remains elusive. Threonylcarbamoyladenosine dehydratases (Tcd) have been proposed as crucial players for the enzyme-dependent dehydration of t6A to ct6A, but their structural and biochemical characterization is missing. Methodological focus: Construct design, molecular biology methods (PCR, cloning, mutagenesis, transformation in bacteria and yeast, plasmid preparation), production of knockouts in yeast, in vivo tagging of yeast genes, protein expression (E. coli and yeast) and purification (IMAC, SEC, IEX), ESI full-length MS for protein verification, structural protein characterization (HPLC, AUC, SAXS), SDS-PAGE (Coomassie staining), EMSA for RNA detection (SYBRGold staining), in vitro transcription of tRNAs, tRNA sequencing, Thermofluor assay, Western blotting Main focus: Crystallography ( handling of crystallization robots, selection of crystallisation conditions, seeding, crystal fishing, measurement of data at PSI, data evaluation) Collaboration with other chairs in the fields of electron microscopy, SAXS, NMR, AUC, NGS, mass spectroscopy Used Software: Snapgene, Unicorn(Äkta), Gimp, Pymol, CorelDraw, Coot, CCP4, Supervision of trainees, study-relevant research internships as well as practical courses Peer review of manuscripts in scientific journals Show less



Technical University of Munich

• Germany
• Research Services
• 700 & Above Employee

• Master Thesis Student at the Chair of Virology

  • Oct 2017 - May 2018

  Topic: Role of host cell immunological regulatory proteins during adenoviral infection Topic: Role of host cell immunological regulatory proteins during adenoviral infection



Technical University of Munich

• Germany
• Research Services
• 700 & Above Employee

• Research Intern at the Chair of Food Chemistry

  • Apr 2017 - May 2017

  Chemistry minor practical course Analytical chemistry, sample prepartion and LC-MS/MS Chemistry minor practical course Analytical chemistry, sample prepartion and LC-MS/MS



Technical University of Munich

• Germany
• Research Services
• 700 & Above Employee

• Research Intern at the Chair of Virology

  • Oct 2016 - Nov 2016

  Biological minor practical course Cell culture, qPCR, Hepatitis virus Biological minor practical course Cell culture, qPCR, Hepatitis virus



Technical University of Munich

• Germany
• Research Services
• 700 & Above Employee

• Research Intern at the Chair of Biotechnology

  • May 2016 - Jul 2016

  Practical course, protein production, protein purification, native gelelectrophoresis

• Tutor

  • Feb 2015 - Jul 2016

  Tutor for the lecture Molecular Structures and Spectroscopy

• Working Student at the Chair of Biophysics

  • Jan 2016 - Mar 2016

  Protein purification and characterisation



Norwegian University of Science and Technology (NTNU)

• Norway
• Higher Education
• 700 & Above Employee

• Research Intern

  • Oct 2015 - Nov 2015

  Department of Clinical and Molecular Medicine,Prof. M. Børset lab, Bone marrow microenvironment in patients with multiple myeloma -Cell culture -Mitochondira Isolation of mammalian cells -SDS PAGE -FACS Department of Clinical and Molecular Medicine,Prof. M. Børset lab, Bone marrow microenvironment in patients with multiple myeloma -Cell culture -Mitochondira Isolation of mammalian cells -SDS PAGE -FACS