Julia Abrego
Research Technician at Caltech- Claim this Profile
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Spanish Elementary proficiency
Topline Score
Bio
Experience
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Caltech
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United States
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Research Services
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700 & Above Employee
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Research Technician
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Jul 2022 - Present
• CRISPR-Cas 9 mediated gene editing -Designing guide RNAs -Screen CRISPR edited plant lines • Develop novel sentinel plants that can communicate with bacteria in order to act as a read out for conditions in the soil and environment. -Designing guide RNAs -Cas9 mediated gene editing -Screen CRISPR edited plant lines -Greenhouse skills * Soil preparation, seed preparation/germination, transplanting • Study gene function in Arabidopsis and visualize responses to environmental stimuli -Designing and constructing T-DNA destination vectors by isothermal assembly *PCR, Gibson Assembly, bacteria transformations, plasmid miniprep, plasmid maxiprep, restriction digest, gel electrophoresis, gel extraction, ligation reactions, LR reactions, media prep *Autoclave Show less
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Curative
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United States
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Hospitals and Health Care
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700 & Above Employee
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Senior Research Associate
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Apr 2022 - Jul 2022
Developing a rapid Sepsis diagnostic assay• Nanopore Sequencing• RT and qPCR mastermix preparation -Oligonucleotide bead capture optimization -Including on human blood/ serum samples• LNA primer design optimization• Agilent Tape Station, Qubit
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Clinical Lab Assistant II
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Jul 2020 - May 2022
• Performing PCR on patient samples to help detect the presence of COVID-19• Automated equipment: Integra Viaflo, Agilent Bravo, Multidrop Reagent Dispenser, Biorad CFX96/384, Hamilton Liquid Handler, Roche Lightcycler, Abbot Alinity• Environmental sampling check for contamination• Cross trained in the Plating and Extraction departments
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San Francisco State University
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United States
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Higher Education
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700 & Above Employee
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Team Leader at Science Coding Immersion Program
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Jun 2020 - Aug 2020
• Facilitated zoom team meetings and practiced/ learned code using the Udacity course: Data Analysis with R • Collaborated with team and the Code Lab at San Francisco State University • Tidied up data frames, visualized/analyzed data. • Experience with ggplot2, dplyr, R Markdown and others. • Facilitated zoom team meetings and practiced/ learned code using the Udacity course: Data Analysis with R • Collaborated with team and the Code Lab at San Francisco State University • Tidied up data frames, visualized/analyzed data. • Experience with ggplot2, dplyr, R Markdown and others.
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California State Polytechnic University-Pomona
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United States
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Higher Education
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700 & Above Employee
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SPIRES Scholar
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Aug 2019 - May 2020
• Screen C92 mutants in E. coli • Mutants created using site-directed PCR mutagenesis and the constructs will be expressed in E. coli. The mutant will then be tested in the Sulfolobus expression system to determine if the mutant protein is capable of producing pyramid structures in the cellular host • Screen C92 mutants in E. coli • Mutants created using site-directed PCR mutagenesis and the constructs will be expressed in E. coli. The mutant will then be tested in the Sulfolobus expression system to determine if the mutant protein is capable of producing pyramid structures in the cellular host
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San Francisco State University
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United States
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Higher Education
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700 & Above Employee
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Summer Intern
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May 2019 - Aug 2019
Project1 • Used R coding language to analyze the diversity of drug resistant and susceptible Pseudomonas aeruginosa strains. • Findings were presented at two symposiums at San Francisco State University. • Involved collaboration with a team of students analyzing drug resistance in other microbes Project 2 • Determined which social media platform was most effective in reaching students facing food and housing disparity. • Reached out to former students that have struggled with these issues and asked what services they would have liked to know about. • Each member of the research team was assigned a different social media platform (i.e. Twitter, Instagram, Facebook) to which they posted the same agreed upon content. Show less
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Education
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California State Polytechnic University-Pomona
Biology, Biology, General -
Universidad Austral, Cusco Peru
Climate Change -
Rio Hondo College
Associate of Science - AS, Biology, General