Experience

Roche Sequencing USA

• United States
• Biotechnology Research
• 100 - 200 Employee

• Senior System Engineer

  • Oct 2020 - Present

  -Works hands-on to develop instrument/platform/assay on automated liquid handlers -Troubleshoot systems and provide root causes of complex HW-SW-Assay interaction problems -Communicate test results and corrective recommendations to the team. -Integrates and creates programs to perform assay workflows on liquid handling robots -Works independently with general management direction, conduct hands-on laboratory experiments independently, and apply advanced scientific knowledge to complete assigned tasks and projects. -Assesses results for applicability, validity, and conformance to Product Requirements. -Works closely with external and internal design teams to understand and resolve product performance issues. -Applies advanced technical writing skills to produce reports, and documents. -Serves as a resource to other departments. Participates in the expeditious transfer of products and technologies by providing scientific knowledge and assistance. -Contributes to decisions that impact project team(s) and/or broader organization. In appropriate decisions may have an impact, causing a delay in operations, a financial expense, or customer or user dissatisfactions. -Developed and launched of the AVENIO Edge system/software/reagents Show less



Roche sequencing Solutions

• Scientist II

  • Sep 2017 - Oct 2020

  Reagent development (Feb, 2019 - Present) -Contributed to cross-functional assay and systems development of open workflows and automation solutions for NGS / hybrid-capture applications. -development-wide logistics coordinator for bulk reagent needs -designed and developed automated workflows -created and implemented specific bioinformatic optimizations for assay needs Oncology Assay Development (Jun, 2018 – Feb,2019) -Optimized and developed hybrid capture and deep-sequencing assays for applications in oncology -Analyzed assay performance throughout the workflow -Regular work in experimental design -Evaluated external manufacturers for business development and competitive analysis -Cultivated a knowledge base in hybrid capture and deep-sequencing oncology applications and diagnostics -Early development work -collaborates with bioinformatics for advanced data analysis -Managed the set-up and maintenance of a protected directory for clinical data Medical & Scientific Affairs (Sep, 2017 – Jun, 2018) -Operated in a cross-functional team that is seeking to provide medical value for new sequencing assays for use in oncology. -Dealt with clinical plasma and FFPET samples -Managed samples across a five-day manual workflow (isolation, Library preparation, target enrichment pooling and sequencing on Hiseq4000). -Performed QC assays/analysis throughout the workflow (QuBit, Tapestation, Bioanalyzer, and qPCR) -Performed actions within a clinical setting following GXP guidelines -Responsible for maintaining and ordering reagents from multiple vendors -Assisted in development of a LIMS system for laboratory workflows -Developed internal forecasting and budgeting tools Show less



Thermo Fisher Scientific

• United States
• Biotechnology Research
• 700 & Above Employee

• Research Associate

  • Jan 2016 - Sep 2017

  [Formerly Affymetrix®] -High throughput AXIOM®, GeneCHIP®, WT Pico Assay (Whole Transcript expression), OncoScan® genotyping methods using common sample types (FFPE, fresh frozen samples, etc.) -Monitoring and operating: FXp® (by Beckman Coulter),GeneTitain® (Affymetrix), GeneCHIP® Fluidics Station (FS) 450, and Integra Viaflo (384) instruments. -Editing and drafting of SOP -Assisted in Product development projects for prototype instruments and workflows -Performed Quality Control assays for all samples -Comfortable with liquid microrrays used in next-generation sequencing (Illumina nextseq 550/500). -Familiar with Quantigene® plex assay (RNA quantification) Show less



San Jose State University

• United States
• Higher Education
• 700 & Above Employee

• Student

  • Aug 2015 - May 2017

  *see education and Projects* *see education and Projects*



Self-employed

• California State University - East Bay

• Lab Researcher

  • 2012 - Aug 2014

  SNP Analysis (Nicotinic Acetylcholine Receptor) -DNA fragment Analysis (Gel electrophoresis) -restriction digests -DNA quantification (RT-PCR, spectrophotometer) -Next-Generation Sequencing -Sanger Sequencing -Bioinformatics analysis (Base-calling, BLAST, database seasrching, SNP identification) -cell culturing (human) -Hemocytometer Bioremediation: -Ion Torrent (Thermo fisher) NGS experience and library preparation -AAS -Calculations (ppm/ppb concentrations) -cell culture (bacteria and algae) -FT-IR analysis -DNA sequencing Show less