Joseph Requejo
Manufacturing Associate at AIVITA Biomedical- Claim this Profile
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Bio
Experience
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AIVITA Biomedical, Inc.
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United States
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Biotechnology Research
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1 - 100 Employee
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Manufacturing Associate
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Jan 2019 - Present
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UCI School of Medicine
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United States
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Higher Education
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500 - 600 Employee
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Staff Research Associate I
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Jul 2016 - Oct 2018
I worked in Dr. Aileen Anderson lab and worked under project scientist Dr. Katja Piltti. The overall lab objective is to use neural stem cells to replace or repair circuity after spinal cord injury, and understand how these neural stem cells are affected by the inflamed spinal cord microenvironment. In particular I investigated how the complement protein C1q affects neural stem cells in-vitro and how a C1q neutralizing antibody injected in T9 spinal cord injured mice could affect neural stem cell proliferation and differentiation, RNA and protein expression, and locomotor behavior. During this time I developed skills in experimental planning, sterile Xeno-Free Human Neural Stem Cell culture, immunocytochemistry, confocal microscopy and imaging, bladder care and injections for spinal cord injured mice and rats, perfusions and tissue extraction, DNA/RNA/Protein extraction, qPCR, Western Blot, ELISA, immunohistochemistry, FLO Cytometry, Imagestream and data analysis in Prism 5. Show less
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California Institute for Regenerative Medicine (CIRM)
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United States
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Biotechnology Research
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1 - 100 Employee
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CIRM Bridges to Stem Cell Research Intern - Dr. Aileen Anderson
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Jan 2016 - Jul 2016
I worked as a paid intern at University California, Irvine under Dr. Aileen Anderson. During this internship, I investigated whether an age-related increase of complement protein C1q in the mouse spinal cord alters the number of newly generated spinal cord cells and their lineage selection using C1q knockout and C1q wild type mouse model. Under CIRM, I designed a research proposal that provided experimental design and scientific rationale to support the experiments to be completed. Results suggest no difference in total number of newly born cells and no difference in total number of newly born mature oligodendrocytes cells between aged C1q WT and C1q KO mouse spinal cord. However, in aged C1q KO mice, there were more newly born cells adjacent to the ependymal stem cell niche compared to WT littermates that may suggest C1q effect on NSC mobilization from the central canal/ependymal stem cell niche. Show less
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Education
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California State University-Fullerton
Bachelor of Science - BS, Cellular and Developmental Biology